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Bacillus for antagonizing fusarium wilt and promoting growth and application thereof

A technology of bacillus and fusarium wilt, applied in the field of microorganisms, can solve the problem of poor control effect of plant fusarium wilt

Active Publication Date: 2020-04-10
福建省农业科学院农业生物资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these bacteria do not have a good control effect on plant wilt

Method used

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  • Bacillus for antagonizing fusarium wilt and promoting growth and application thereof
  • Bacillus for antagonizing fusarium wilt and promoting growth and application thereof
  • Bacillus for antagonizing fusarium wilt and promoting growth and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening of antagonistic strains

[0028] 1. Soil sample collection and Bacillus isolation and purification

[0029] The grassland soil samples near Bayong, Shenzha County, Tibet were made into soil suspension, which was then properly diluted with sterile water and coated on LB plates (LB: tryptone 10g L -1 , sodium chloride 10g·L -1 , yeast powder 5g·L -1 , agar powder 1.5%, pH 7.0-7.2); cultivated at 30°C for 48 hours, picked a single colony, stored in glycerol, and used it as the strain to be tested. 60 strains were isolated and purified by dilution plate method as the strains to be tested.

[0030] 2. Screening of fusarium wilt biocontrol strains

[0031] (1) Preliminary screening by confrontation method

[0032] The tested strains were activated on LB plates and cultured at 30°C for 48 hours.

[0033] Fusarium oxysporum FJAT-30512 (Chen Qianqian, Liu Bo, Liu Guohong, Che Jianmei, Gong Haiyan. Study on the Diversity of Geobacillus in the Rhizosphere...

Embodiment 2

[0057] Identification of embodiment 2 antagonistic strains

[0058] 1. Morphological characteristics

[0059] The Bacillus sp. FJAT-47158 was activated on the LB plate, and the colony shape, size, color, transparency, protrusion and edge of the strain were observed after 48 hours.

[0060] The colony characteristics of strain FJAT-47158 are as follows figure 1 As shown, round, pale yellow, neat edges, wrinkled surface, opaque, viscous medium-sized colonies.

[0061] 2.16S rRNA gene sequence analysis

[0062] Genomic DNA of strain FJAT-47158 was extracted by Tris-saturated phenol method, PCR amplification was carried out using 16S rRNA gene general primers 27F and 1492R, and the PCR reaction procedure was referred to the literature of Zheng Xuefang et al (Zheng Xuefang, Liu Bo, Zhu Yujing, et al. Screening and identification of biocontrol Bacillus [J]. Chinese Journal of Biological Control, 2016, 32(5): 657-665.), the PCR product was sent to Shanghai Boshang for sequencing, ...

Embodiment 3

[0064] The inhibitory effect of embodiment 3 bacillus to Xanthomonas rugosa

[0065] Inoculate the FJAT-47158 strain on the LB plate, and then pick a single bacterial colony and transfer it to 10mL LB liquid medium, 170r min -1 , cultured at 30°C for 48 hours, and counted by hemocytometer, the colony density of FJAT-47158 reached 2.352×10 8 cfu mL -1 . The FJAT-47158 fermentation broth was centrifuged at 4000rpm for 8min, the supernatant was taken, and the bacterial cells were discarded.

[0066] 1mL of the bacterial suspension of Xanthomonas rugosa FJAT-10151 (preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, preservation number CGMCC NO.10271) (colony density 4.224×10 8 cfu mL -1 ) mixed with 200mL of 50°C NA semi-solid medium (3g of beef extract, 5g of peptone, 10g of glucose, 9g of agar, and 1L of water), draw 4mL and pour it into the prepared NA solid medium (3g of beef extract, 5g of peptone, Glucose 10g, ...

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Abstract

The invention provides bacillus for antagonizing fusarium wilt and promoting growth and an application of the bacillus. The bacterial strain is bacillus simplex FJAT-47158, and the chemical name of the bacterial strain is Bacillus simplex FJAT-47158. The bacterial strain is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the bacterial strainis CGMCC NO. 15946. The bacillus disclosed by the invention not only can inhibit phytopathogens such as fusarium oxysporum, xanthomonas carpetalis and the like, but also can improve the activity of plant seeds and promote the growth of the seeds, can be used for preventing and treating fusarium wilt, and is an environment-friendly, economical and effective biocontrol bacterium for preventing andtreating diseases.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a bacillus strain which antagonizes fusarium wilt and promotes growth. Background technique [0002] Fusarium wilt is a systemic infection disease, which is a common soil-borne disease of plants such as Lymegmus, soybean, wheat, tomato, cotton, strawberry, etc. It is an increasingly prominent problem in the United States, Canada, Australia, and China. Due to Fusarium wilt, the leaves become smaller, lack luster, the branches are dwarfed, the fruits are thin, or the leaves turn yellow and wilt. In severe cases, the plants gradually die. The main pathogenic populations of Fusarium wilt are Fusarium oxysporum and Fusarium solani. The control methods include chemical reagents, fungicides, biological control, phytotherapeutic drugs, optimized fertilization, etc., and the use of biocontrol bacteria to control wilt Disease prevention is an important measure of comprehensiv...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCC12R2001/07C12N1/205Y02A40/10
Inventor 朱育菁郑梅霞陈峥刘波许炼潘志针肖荣凤邓文琼李慧敏
Owner 福建省农业科学院农业生物资源研究所
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