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Bionectria ochroleuca Bo-1 strain, cultures thereof, and applications of the strain and the cultures thereof in resisting pathogenic bacteria

A kind of anti-pathogen technology of Rhodochrochrome light-colored, applied in the field of agricultural production of microbial technology, can solve the problem of few types of microbial source pesticides, etc., and achieve the effect of broad application prospects

Active Publication Date: 2013-08-07
郎溪品旭科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems of unsafe chemical pesticides and fewer types of microbial source pesticides, the first object of the present invention is to provide a kind of Echrochium erythrosum ( Bionectria ochroleuca) Bo-1 bacterial strain, the second object of the present invention is to provide fermented liquid and crude extract thereof of Echroleuca ochroleuca light color, the third object of the present invention is to provide light color erythroleuca Shell fungus Bo-1 bacterial strain or the method that the culture of this bacterial strain is used for antipathogenic bacterium, the 4th object of the present invention is to provide a kind of biological antipathogenic bacterium preparation

Method used

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  • Bionectria ochroleuca Bo-1 strain, cultures thereof, and applications of the strain and the cultures thereof in resisting pathogenic bacteria
  • Bionectria ochroleuca Bo-1 strain, cultures thereof, and applications of the strain and the cultures thereof in resisting pathogenic bacteria
  • Bionectria ochroleuca Bo-1 strain, cultures thereof, and applications of the strain and the cultures thereof in resisting pathogenic bacteria

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Experimental program
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Effect test

Embodiment 1

[0037] Isolation and screening from the inside of healthy rice leaves in a paddy field yielded a strain that was antagonistic to bacterial blight of rice B. ochroleuca ) Bo-1 strain.

[0038] (1) Plant pathogenic bacteria tested

[0039] Xanthomonas oryzae ( Xanthomonas oryzae pv. oryzae, Xoo ) P6 physiological race.

[0040] (2) Medium

[0041] Potato Dextrose Agar Medium (PDA): Potato (200 g), Glucose (20 g), Agar (15-20 g), Water (1000 mL), pH 6.0~6.5.

[0042] Potato glucose liquid fermentation medium (PD): potato (200 g), glucose (20 g), water (1000 mL), pH 6.0~6.5.

[0043] Improved PDA solid medium: potato (300 g), peptone (5.0 g), sucrose (15.0 g), Ca(NO 3 ) 2 4H 2 O (0.5 g), NaHPO 4 12H 2 O (2.0 g), agar (15.0 g), water (1000 mL), pH 6.0~6.5.

[0044] Improved PD liquid medium: potato (300 g), peptone (5.0 g), sucrose (15.0 g), Ca(NO 3 ) 2 4H 2 O (0.5 g), NaHPO 4 12H 2 O (2.0 g), water (1000 mL), pH 6.0~6.5.

[0045] (3) Experimental method

...

Embodiment 2

[0061] Erythrochromosporum ( B. ochroleuca ) Inhibitory effect of Bo-1 strain fermented crude extract on common pathogenic bacteria.

[0062] (1) The tested plant pathogenic bacteria: Escherichia coli ( Escherichia coli ), Candida albicans ( Monilia albican ), Bacillus subtilis ( Bacillus subtilis ),salmonella( Salmonella ),Shigella( Shigella ), Listeria ( Listeria monocytogenes ), Staphylococcus aureus ( Staphyloccocus aureus ).

[0063] (2) Medium: Beef extract peptone medium, Half Fraser broth medium, buffered peptone water medium, GN enrichment medium.

[0064] (3) Experimental method: The above-mentioned pathogenic bacteria to be tested were respectively inoculated in the corresponding liquid medium shaker (120 r / min) for activation culture. When the concentration of the bacterial suspension reached OD value 0.6, 100 μL of the bacterial liquid was evenly spread on the corresponding solid medium , stand-by; use 2% acetone to make a 100 μg / mL solution ...

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Abstract

The present invention relates a plant endophyte Bionectria ochroleuca Bo-1 strain, cultures thereof, and applications of the strain and the cultures thereof in resisting pathogenic bacteria. The strain is preserved in China Center for Type Culture Collection (CCTCC), the address of the center is Wuhan University, Wuhan, China, the accession number is CCTCCNO: M2013027, and the preservation date is January 18, 2013. The Bionectria ochroleuca Bo-1 strain of the invention has high resisting performance for Xanthomonas campestris pv.oryzae, and a strong inhibitory effect on Monilia albicans (M.albican), Escherichia coli (E. coli), Staphylococcus aureus (S. aureus ), Bacillus subtilis (B. subtilis) and other pathogenic bacteria.

Description

technical field [0001] The invention belongs to the field of agricultural production of microbial technology, and in particular relates to the endophytic fungus Pleurotus erythrosum ( Bionectria ochroleuca ) Bo-1 strain and its culture and application in antagonizing pathogenic bacteria. Background technique [0002] Rice bacterial blight is one of the main diseases in my country's rice production, which can lead to a 20%-30% reduction in rice, and even grain failure in severe cases. Rice bacterial blight, also known as white leaf blast, thatch blast, ground fire, etc., is caused by Xanthomonas oryzae ( Xanthomonas oryzae pv. oryzae, Xoo ), which occurs in various rice areas in my country and is the main disease of rice. The main way of prevention and control at present stage is chemical control, but excessive application of Chikujing, Thiacumazole and other chemicals will lead to enhanced resistance of pathogenic bacteria and serious damage to the ecological environme...

Claims

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Application Information

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IPC IPC(8): A01N63/04C12N1/14A01P1/00C12R1/645
Inventor 蒋冬花刘琴英翁建超谢斯超吴鹏潘红影
Owner 郎溪品旭科技发展有限公司
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