80 results about "Fructose-bisphosphate aldolase" patented technology

The regulatory sequences (i.e., promoter regions, introns and enhancers) associated with the Yarrowia lipolytica gene encoding fructose bis-phospate aldolase (FBA1) have been found to be particularly effective for the expression of heterologus genes in oleaginous yeast. The promoter regions of the invention have been shown to drive high-level expression of genes involved in the production of ω-3 and ω-6 fatty acids.

The present invention relates to a method for producing optically active IHOG, which can in turn be used for the production of monatin. The present invention further relates to a method for producing optically active monatin, and aldolase used for these methods. As such, the present invention enables the synthesis of 4-(Indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid with high optical purity, which is useful as an intermediate in the synthesis of optically active monatin, from indole pyruvic acid and pyruvic acid (or oxaloacetic acid).

4-(Indol-3-ylmethyl)-4-hydroxy-2-oxoglutarate, which is useful as an intermediate in the synthesis of monatin, may be synthesized from indole pyruvic acid and pyruvic acid (and / or oxaloacetic acid) by using a novel aldolase derived from the genus Pseudomonas, Erwinia, Flavobacterium, or Xanthomonas.

A novel aldolase is described. 4-hydroxy-3-methyl-2-keto-pentanoic acid, which is useful as an intermediate in the synthesis of 4-hydroxy-L-isoleucine, may be synthesized from acetaldehyde and α-ketobutyric acid using a novel aldolase, which is derived from the genus Arthrobacter.

The invention discloses a method for synthesis of D-psicose by microbiological fermentation, specifically a method for synthesis of D-psicose by aldolase whole cell. The method includes: firstly constructing recombinant corynebacterium glutamicum SY12 carrying L-fucose-1-phosphate aldolase gene and fructose-1-phosphorylase gene (i.e. with aldehyde condensation approach), then adding glucose and D-glyceraldehyde into a basic salt medium, synthesizing dihydroxyacetone phosphate from glucose by means of intracellular glycolysis, synthesizing a single product D-psicose from D-glyceraldehyde and dihydroxyacetone phosphate (DHAP) under the action of the L-fucose-1-phosphate aldolase and fructose-1-phosphorylase carried by the corynebacterium glutamicum recombinant strain SY12, with the conversion rate of D-glyceraldehyde being 53%. Therefore, compared with the existing method for in vitro synthesis of D-psicose by ketose3-epimerase, the biosynthesis method of D-psicose provided by the invention has the advantages of high conversion rate, single product, easy separation and the like, and lays certain foundation for mass production of D-psicose.

The invention discloses a method for preparing levodopa with a one-pot enzymatic method. The method is characterized in that formaldehyde, glycine and catechol are taken as substrates; formaldehyde and glycine are subjected to a catalytic reaction in the presence of aldolase and other enzymes to be converted into serine, then, serine is converted into pyruvic acid and ammonia under the action of anhydrase, and finally, pyruvic acid and catechol react under the action of tyrosine phenol lyase to produce levodopa. The method adopts novel catalysis routh design and has the advantages of being simple to operate, short in production cycle, low in production cost, high in yield, low in environmental protection pressure, suitable for mass industrial production and the like.

The invention provides a chassis system for ATP (Adenosine Triphosphate) regeneration and application. The chassis system for ATP regeneration comprises the following five enzymes: alpha-glucan phosphorylase, glucophosphomutase, phosphoglucose Isomerase, phosphoketolase and acetokinase. In addition, the chassis system can further comprise the following seven enzymes: transaldolase, transketolase,ribose-5-phosphate isomerase, ribulose-3 epimerase, triosephosphate isomerase, fructose-bisphosphate aldolase and fructose-1,6-bisphosphatase. The chassis system can be coupled to an enzymic catalyticreaction needing ATP, utilizes starch or maltodextrin as energy, is not added with any coenzyme, can efficiently regenerate the ATP at low cost through a one-pot reaction and is an economical, highlyefficient, stable and sustainable ATP regeneration system.

The invention relates to compounds with selective inhibitory activity against aldolase and a preparation method thereof, pharmaceutical compositions containing the compounds, and application of the compounds to preparation of drugs for inhibiting the synthesis of triglyceride and cholesterol, drugs for reducing the synthesis of fatty acid, drugs for preventing and / or treating obesity and type 2 diabetes, drugs for preventing and / or treating tumors, drugs for preventing and / or treating Parkinson's disease, drugs for preventing and / or treating Alzheimer's disease, or drugs for prolonging the life of mammals. The general structure of the compounds is as described in the specification.

The invention relates to a biosynthesis method of 2-deoxy scarce aldose such as 2-deoxy-D-ribose aldolase by using aldolase, and discloses protein sequences of two 2-deoxy-D-ribose 5-phosphate aldolase mutants, and a constructed Escherichia coli recombinant bacterial strain L1 containing encoding genes of the 2-deoxy-D-ribose 5-phosphate aldolase mutants. Experiments show that the recombinant strain can catalyze reaction of acetaldehyde and a variety of aldehyde groups to produce 2-deoxy aldose by using resting cells, and has strong substrate tolerance. For example, recombinant strain can synthesize 2-deoxy-D-ribose by using aldehyde and D-glyceraldehyde as substrates, synthesize 2-deoxy-L-ribose by using acetaldehyde and L-glyceraldehyde as substrates, and synthesize 2-deoxy-D-altrose by using acetaldehyde and D-erythrose as substrates. Therefore, the Escherichia coli recombinant bacterial strain L1 provided by the invention can be applied to the production of deoxidation deoxy scarce aldose, and the obtained deoxy aldose has wide application prospect in the industries of food and medicine.

The invention discloses a cubilose acid aldolase mutant as well as a coding gene and application thereof. The cubilose acid aldolase mutant is obtained from sequence 2 in a sequence list through point mutation, and the point mutation is at least one mutation at the 25th site and 275th site of the sequence. Through site directed mutation on the cubilose acid aldolase, cubilose acid aldolase mutant with high catalytic activity is finally obtained. Besides, the mutant uses N-acetylmannosamine, sodium pyruvate and trinosin (ATP) as the substrate and has the cubilose acid aldolase catalytic activity which is at least 50% higher than that of the parent. Thus, the cubilose acid aldolase mutant can be used for producing cubilose acid (sialic acid), the production cost is lowered, and the market competitiveness of the corresponding product is enhanced.

This disclosure relates to aldolase, an aldolase mutant, and a method and a composition for producing tagatose by using the same. The feature of the disclosure is environment-friendly due to the use of an enzyme acquired from microorganisms, requires only a simple process of enzyme immobilization, uses a low-cost substrate in a substrate compared with a conventional method for producing tagatose and has a remarkably high yield, thereby greatly reducing production costs and maximizing production effects.

The invention discloses a branched ketose method. According to the invention, the new function of known aldolase is provided, wherein the aldolase can catalyze an aldol condensation reaction between dihydroxyacetone phosphate and dihydroxyacetone to synthesize branched ketose, so that the invention discloses a method for synthesizing branched ketose by constructing an in-vitro multi-enzyme cascadereaction system to catalyze dihydroxyacetone on the basis, and the conversion rate is 90%; the invention also discloses a construction method of a corynebacterium glutamicum recombinant strain for producing branched ketose, wherein the branched ketose is synthesized by fermenting a cheap substrate glucose or glycerol with the obtained recombinant strain, and the yield reaches 36.3 g / L; and compared with the existing chemical method for synthesizing branched ketose, the branched ketose synthesis method provided by the invention has the advantages of cheap substrate, environmental friendliness,high product synthesis efficiency, single product and convenience in separation, has industrial application potential, and provides a basis for synthesis of 4-hydroxymethylfurfural.

The invention provides a diesel oil improver component containing biological enzyme, which comprises cetane number improver and biological enzyme, wherein the weight part ratio of the cetane number improver to the biological enzyme is 1:(0.01-1); the biological enzyme is the mixture of sulpho enzyme, alcohol oxidase, aldehyde oxidase, amine oxidase, sulfhydryl oxidase, methyl mercaptan oxidase, methyl naphthoquinone oxidase, peroxidase, ferrochelatase, decarboxylase, aldolase, oxyacid lyase, demethylase, alcohol dehydratase, acid dehydratase, esters dehydratase, desulfhydrase, desulfurization methyl enzyme, cobalt chelating enzyme and magnesium chelating enzyme. According to the diesel oil improver component containing the biological enzyme, provided by the invention, the high speed catalytic performance can be realized under the temperate environment through utilizing properties of the biological enzyme, and the component is combined with the conventional cetane number improver for use, the catalytic combustion efficiency of the cetane number improver in diesel oil is greatly improved, and the combustion performance of the diesel oil is further improved.

The invention discloses an L-threonine aldolase mutant and a method for preparing L-syn-p-methylsulfonyl phenyl serine. The L-threonine aldolase mutant is obtained by mutating wild type L-threonine aldolase, and L-syn-p-methylsulfonyl phenyl serine can be generated by a catalytic condensation reaction by taking glycine and p-methylsulfonyl benzaldehyde as substrates and taking pyridoxal phosphate as a coenzyme. The advantages of using the L-threonine aldolase mutant for producing L-syn-p-methylsulfonyl phenyl serine are that 1, the production process is simple, and the reaction conditions are mild; 2, the selectivity of the L-threonine aldolase is high, the optical purity of the product is high, and resolution is not needed; 3, the atom utilization rate is high and can reach 100% theoretically; 4, the production process is environmentally friendly, pollution is small, and the green chemistry concept is met; and 5, the product is simple to separate and purify.

The invention discloses a method for increasing the yield of sialic acid and an application, and belongs to the field of enzyme engineering and microbial engineering. According to the invention, the enzyme catalytic activity key amino acid site Gly371 of N-acetylglucosamine-2-epimerase is subjected to saturated mutation, and an enzyme mutant capable of improving the catalytic efficiency is screened. Through overexpression of the N-acetyl glucosamine-2-epimerase mutant and the N-acetylneuraminic acid aldolase with improved catalytic efficiency, the production efficiency of the recombinant escherichia coli for catalytic production of sialic acid is improved. The yields of Neu5Ac prepared by whole-cell catalysis of the recombinant escherichia coli containing G371C and the recombinant escherichia coli containing G371A, which are constructed by the invention, are respectively 352.0 and 353.6 mmol.L <-1 >, which are obviously higher than those of wild recombinant bacteria. Besides, the conversion efficiency of the Neu5Ac generated by converting GlcNAc under catalysis of a recombinant strain containing a mutant is obviously higher than that of a control strain.

In one aspect, the present invention relates to a method of identifying compounds useful in modifying the activity of Aldolase. The method includes providing a first model comprising Aldolase or residues of the amino acid sequence corresponding to SEQ ID NO: 1 said residues being at amino acid positions selected from the group consisting of 10-13, 26, 27, 29, 30, 31, 32, 33, 37, 39, 40, 41, 43, 44, 47, 48, 51, 52, 60, 63, 66, 79, 84, 85, 92, 93, 103, 106-109, 112-117, 138, 142, 146, 148, 151, 153, 179, 182, 183, 185, 186, 194, 196, 197, 198, 199, 208, 226-228, 231-269, 270, 272, 277-283, 285-289, 294, 295, 297-299, 301-304, 306-310 312, 313, 316, 317, 319, 321, 323, 326, 330, 344, 345, and 347, providing one or more candidate compounds, evaluating contact between the candidate compounds and the first model to determine which of the one or more candidate compounds have an ability to bind to and / or fit in the first model, and identifying compounds which, based on said evaluating, have the ability to bind to and / or fit in the first model as compounds potentially useful for modifying the activity of Aldolase. The present invention also discloses compounds and compositions which modify the activity of Aldolase, or a complex between Aldolase and TRAP. Methods of treating or preventing malaria, or an infection by apicomplexan organisms are also disclosed.