34 results about "Furtadoa" patented technology

The invention discloses a breeding method for aseptic seeding and test tube seedling formation of orchids and the adopted broad-spectrum culture medium. In this method, a healthy orchid mother plant is selected, and artificially pollinated when it blooms, and the fruit that develops to maturity after pollination and has not cracked is used as an explant, or the orchid fruit that is basically mature and not cracked is taken from the wild as an explant. Implants undergo reproductive steps such as aseptic sowing, seed germination, strong seedling cultivation, and test-tube seedling transplantation. The specially prepared medium is used for aseptic sowing and seed germination to obtain small plants, and then the small plants are cultivated on the specially prepared strong seedling medium, and the seedlings are hardened under natural light in the greenhouse for 7-14 hours before being released from the bottle. One day, the test-tube seedlings were taken out, the root medium was washed off, and they were planted in a mixed substrate of bark, bluestone and peat, and further cultivated into seedlings. The invention has the characteristics of high seed germination rate, fast seedling growth, good seedling quality, low cost, etc., can obtain a large number of test-tube seedlings in a short period of time, and the transplanting survival rate of the seedlings can be kept above 90%. An effective way can be provided for the production of orchid seedlings.

The present invention relates to in planta transformation methods for plants or plant materials with a genetic construct, wherein at least one meristematic cell of a meristematic tissue of an immature inflorescence able to differentiate into a gamete of a pollen or of an ovule is exposed and then transformed, wherein the transformation can be performed to yield a stable integration or to yield a transient introduction of a genetic construct of interest. Further provided are methods for manufacturing a transgenic plant, or methods for manufacturing a genetically manipulated plant based on the in planta transformation methods according to the present invention. In addition, there is provided a plant or a progeny thereof manufactured according to the transformation and / or manufacturing methods according to the present invention.

The invention relates to the method for identifying the varieties of lilium brownii by adopting an SSR (simple sequence repeat) molecular marking technology. A result shows that: two pairs of high-polymorphism and high-repeatability primers are screened from 13 pairs of primers for detecting 171 fragments comprising 131 polymorphic fragments, 4 to 22 fragments can be detected by each pair of primers with the sizes of 100 to 250bp, 1.69 bands are amplified by each pair of primers, and the polymorphism rate is 76.70 percent; NTSYS-pc2.11 is used for similarity coefficient and cluster analysis, the genetic similarity coefficient is 0.5882 to 09412, and the average similarity coefficient is 0.7647; materials are divided into six major classes by cluster analysis, the genetic differences between 11 lilium brownii germplasms are irrelevant to varietal characteristics, flower forms, the colors of filaments and styluses, but have certain correlation with the colors of petals, flower genes can better reflect genetic relationships between the lilium brownii germplasms, different varieties have different colors, and the primers can be designed by controlling related genes of flowers to provide an important means for further distinguishing the genetic relationships.

The invention discloses a novel double-ant capsule for clearing dampness and dredging collaterals. The novel double-ant capsule comprises the following important raw materials: 10-20 parts of silky ant, 4-8 parts of odontotermes formosanus, 0.5-1.0 part of ginseng, 10-15 parts of astragalus membranaceus, 2-5 parts of bighead atractylodes rhizome, 5-10 parts of angelica sinensis, 1-5 parts of root of red-rooted salvia, 0.5-1 part of radix aconiti preparata, 2-6 parts of caulis spatholobi, 1-5 parts of morinda officinalis, 1-5 parts of herba epimedii, 0.5-1.0 part of radix achyranthis bidentatae, 10-15 parts of radix clematidis, 0.2-0.8 parts of centipede, 0.5-1.0 part of scorpion,1-5 parts of carthamus tinctorius linne, 6-12 parts of fruit of Chinese wolfberry, 1-3 parts of semen coicis, and 3-9 parts of honeysuckle. By adopting the honeysuckle added to the novel double-ant capsule, the effective components of the other 18 traditional Chinese medicines can be effectively extracted; the medicine properties can be furthest volatilized; the used medicinal materials can be fully utilized; furthermore, the effective components of each traditional Chinese medicine are furthest extracted, so that the quantity of the used medicinal materials is relatively reduced, the production cost is also reduced, and the productivity effect is improved.

The invention discloses a castanopsis hystrix pollen collecting and storing method for increasing the germination rate and improving the pollen viability. The method comprises the following steps that when male parent male flowers are in a transitional period of an early blooming period and a full blooming period, male parent male flower inflorescence is collected at 9:00-11:00 or 15:00-17:00; the male flower inflorescence is dried for 12-24 h in a sterile room at the temperature of 22 DEG C-28 DEG C under illumination of 80 W-160 W, when a large amount of anther blows out and a large amount of yellow pollen scatters during gentle shaking, drying is completed; the dried male flower inflorescence is screened with a pollen sieve, pollen is collected and contained in a container, an opening of the container is sealed, and the container is placed in an environment at the temperature of minue 60 DEG C-minus 100 DEG C or liquid nitrogen to be stored. Furthermore, when the pollen needs to be germinated, the stored pollen is placed in a liquid culture medium and can be germinated at the temperature of 25 DEG C-35 DEG C. Accordingly, a castanopsis hystrix pollen collecting, drying, storing and viability detecting system is formed for the first time, the method is simple, rapid and high in operability, the castanopsis hystrix pollen viability can be improved, and the manual cross pollination success rate of castanopsis hystrix can be increased.

The invention relates to plant genes and particularly discloses a novel symmetry gene CmCYC2c of chrysanthemum morifolium and a novel application of the symmetry gene CmCYC2c in the aspect of regulation of ligulate flower petal traits of the chrysanthemum morifolium, especially, in the aspects of increase of the number of ligulate flower petals and increase of petal length. The nucleotide sequence of the symmetry gene CmCYC2c is represented as SEQ ID NO.1. Based on the gene and the application thereof, the CmCYC2c gene is integrated to a chamomile genome with a transgenic technology, the flower type is primarily and directionally improved, so that the number of the ligulate flowers is increased, the petal length is increased, and a novel and practical method is provided for primary selection of new species of chrysanthemum morifolium varieties with the genetic engineering technology.

The invention discloses a planting and breeding combination ecological circulation method for a saccharum officinarum field, and belongs to the ecological agriculture field. The planting and breedingcombination ecological circulation method comprises the following steps of: (1) seed selection; (2) soil preparation and fertilizer application; (3) seeding conduction; (4) field management: a: carrying out reasonable fertilizer application, b: carrying out moisture management and c: thinning; (5) aquaculture: 1) carrying out drainage culture, and 2) carrying out moisture and food management; and(6) harvesting conduction. The planting and breeding combination ecological circulation method disclosed by the invention fully performs the advantages of the stereoscopic agriculture of the saccharumofficinarum field, a stereoscopic planting and breeding mode of the saccharum officinarum field is adopted, Cucumis sativus L. is planted among different pieces of saccharum officinarum, and fishes and Cipangopaludina chinensis Gray are cultured in grooves among furrows. Compared with a traditional saccharum officinarum cultivation way, the planting and breeding combination ecological circulationmethod has a better spatio-temporal allocation among species, and a colony ecological factor is further optimized.

The invention discloses a method for regulating and controlling the flowering stage and flowering traits of cymbidium sinense. According to the method, through fertilizer and water, illumination and temperature management in the flower forcing process, a plant growth regulator mixed solution I is sprayed in the early stage of flower bud differentiation to promote flower bud differentiation to be completed in advance, and a plant growth regulator mixed solution II is sprayed again in the flower arrow elongation stage to promote flower arrow elongation and inflorescence development. By means ofthe method, the number of buds of each pot is 2-3, the number of flowers on each inflorescence is increased and is averagely three more than that of contrast, the flowers become larger, and the initial flowering period can be advanced by 24 days. According to the method, the cymbidium sinense is stabilized to bloom before the Spring Festival, the blooming quality is improved, the arrow is increased and lengthened, the inflorescence is lengthened, the number of flowers is increased, the flowers are enlarged, the ornamental effect is better, a foundation is laid for meeting the huge demand of phalaenopsis orchid, the purposes of further expanding the market and increasing the market share of the cymbidium sinense are achieved, and economic benefits are outstanding.

The invention relates to an interspecific cross breeding method of fagopyrum dibotrys and tartary buckwheat and application. According to the interspecific cross breeding method of the fagopyrum dibotrys and the tartary buckwheat and application, with tetraploid tartary buckwheat as female parents and a diploid fagopyrum dibotrys complex plant doubled line and / or tetraploid fagopyrum dibotrys which naturally exists as male parents, crossing is conducted, and a cross breed obtained by adopting the interspecific cross breeding method of the fagopyrum dibotrys and the tartary buckwheat can live through the winter in warm southern areas, so that years of asexual propagating expanding production and cultivation are achieved. According to the breeding method, not only is the interspecific heterosis of the fagopyrum dibotrys and the tartary buckwheat used, but also the heterosis among different fagopyrum dibotrys lines is used, the perennial characteristic and the multiple regeneration capability of the interspecific cross breed fagopyrum dibotrys are further improved, and thus the regeneration capability of branch cutting and multiple cutting of cross breed plants is strong enough to support overwintering and propagating expanding of multiple branch cutting, so that the heterosis of the cross breed is maintained permanently, and the cross breed can be widely applied and popularized in production.

The invention relates to the field of rumen regulation and control, in particular to a natural product preparation for promoting the rumen fermentation. The natural product preparation is prepared through the following steps: after grinding homalomena occulta roots, extracting with 60-80 mass percent of ethanol solution, and performing nitrogen flushing and freeze drying to obtain dry powder. Through researching, the inventor has discovered that the homalomena occulta roots have a certain effect on promoting the rumen fermentation. Homalomena occulta has a wide source property and cooperativity, the experiment through adopting a filter paper flat band method shows that obscured homalomena rhizome volatile oil has a function of inhabiting bacterium burgeri on flocks and herds. Therefore, the homalomena occulta root extractive preparation is hopeful to further comprehensively improving the rumen function and promoting the health of ruminants after scientific compatibility.

The invention discloses a method for prolonging the refreshing time of borage flowers. The method comprises the following steps: (1) immersing the borage flowers into a sodium alginate solution, then taking out the borage flowers, and draining the residual sodium alginate solution; and (2) immersing the borage flowers into a calcium chloride solution to induce spontaneous cross-linking reaction, then taking out the borage flowers and absorbing moisture on the surfaces of the borage flowers. The preservation time of the borage flowers treated by the steps (1) and (2) is 5-6 days and is 4-5 days longer than the preservation time of untreated fresh cut flowers. The method comprises the step (3) that the borage flowers are inserted in a fresh-keeping solution, wherein the fresh-keeping solution is prepared from a bactericide, a nutritional agent, an ethene antagonist, a plant growth regulator and the like, so the aging of the borage flowers can be further slowed down, the vase life of the cut flowers can be prolonged, and the ornamental quality of the cut flowers can be improved. The preservation period of the borage flowers treated by the steps (1), (2) and (3) is 10-15 days and is 9-14 days longer than that of untreated fresh cut flowers, and a relatively high water activity value can be maintained.

The invention aims to provide an ecological landscape talking machine animal and a manufacturing method thereof. Green ecological landscape traveling garden recreational and cultural products which are high in environment-friendly consciousness and consciousness of cherishing and protecting (machine) animals and plants further and planning (talking assembly) and design speed, short in cultivation time, wide in development area, high in ornamental value, multiple in job placement and good in social and economic benefits and a manufacturing method are provided for people by utilizing the scientific and technical innovation of adding and improving a talking assembly function in an oral cavity of the original machine animal, adding rattan flowers and grass serving as green plants, such as caulis trachelospermi, Chinese ivy, Virginia creeper, ulvaceae, stereocolon, fewflower lysionotus herb, axonopus affinis, herb of prostrate euphorbia, liverwort, fortune euonymus stem leaf, costustoot, Japanese honeysuckle stem, sabina procumbens, jasminum nudiflorum lindley, lobaria pulmonaria, lycopodium pulcherrimum wall., rhododendron simsii, stem and leaf of Henry elaeagnus, tea tree and lycium bark on body surfaces, and cultivating ricciocarpus natan on bodies of animals in Chinese zodiac and pandas, dinosaurs, lions, leopards, deer, wolves, cats, bears, elephants, camels, boars, mastiffs, hawks, geese, birds, turtles, fish, frogs and elaphurus and methods of design, cultivation, hanging, binding, bending, pruning and management.

The invention relates to the field of traditional Chinese medicine external application agents, and particularly discloses an external application agent for treating pelvic inflammation of cold uterustype and a preparation method thereof. The external application agent for treating the pelvic inflammation of the cold uterus type is endowed with the effects of relieving pain and diminishing inflammation in virtue of mutual compatibility of radix angelicae dahuricae, radix dipsaci asperoidis, zanthoxylum schinifolium, ligusticum chuanxiong, cortex acanthopanacis, homalomena rhizome and trberculate speranskia herb, so that the external application agent is capable of effectively diminishing inflammation and removing swelling; the external application agent is endowed with the effects of dispelling wind and eliminating dampness in virtue of mutual compatibility of the zanthoxylum schinifolium, the homalomena rhizome, cortex illicii, Chinese viscum herb, argy wormwood leaves, the trberculate speranskia herb, incised notopterygium rhizome and roots, double-teeth pubescent angelica roots and the ligusticum chuanxiong, so that the external application agent is capable of effectively removing coldness and dampness from the uterus of the patient; and the external application agent is also endowed with the effects of promoting blood circulation for regulating menstruation in virtue of mutual compatibility of the argy wormwood leaves, the trberculate speranskia herb, radix paeoniae rubra, Chinese angelica roots, frankincense, myrrh, common buried rubber, zedoray rhizome and red-rootedsalvia roots, so that the external application agent is capable of effectively promoting blood circulation for regulating menstruation of the patient. Moreover, in virtue of the effects of an absorption enhancer, a ginger brown-sugar preparation and a scallion stalk extract, the external application agent becomes further capable of preventing and treating bacteria in the uterus of the patient.

The invention relates to a medicine composition with a special effect of tonifying yang. The medicine composition is prepared from the following components in parts by mass: 110-130 parts of radix paeoniae alba, 50-70 parts of radix astragali seu hedysari, 40-50 parts of rhizoma drynariae, 40-50 parts of caulis spatholobi, 20-30 parts of herba epimedii, 20-30 parts of radix morindae officinalis, 20-30 parts of radix clematidis, 20-30 parts of herba lycopodii, 20-30 parts of herba speranskiae tuberculatae, 20-30 parts of radix angelicae sinensis, 20-30 parts of rhizoma homalomenae, 15-25 parts of radix glycyrrhizae preparata and 25-35 parts of centipede. According to the medicine composition, radix paeoniae alba, radix astragali, rhizoma drynariae, caulis spatholobi, herba epimedii, radix morindae officinalis and radix clematidis are taken as main medicines, and the seven main medicines are synergistically matched, so that the symptoms such as lumbago, lumbar muscle strain, cramp of legs and feet, flaccid paralysis, rheumatic arthralgia and tendon and vessel spasm are effectively improved, and a yang-tonifying medicinal effect is provided to improve various yang deficiency symptoms; meanwhile, the lycopodium clavatum, the garden balsam stem, the angelica sinensis and the obscured homalomena rhizome are used as ministerial drugs, so that the effects of inducing diuresis, detoxifying, stimulating the menstrual flow, penetrating the stem, enriching and promoting blood circulation and the like are achieved; and finally, honey-fried licorice root and centipede are used as adjuvants, and the detoxification function of the adjuvants is utilized, so that damage to liver and kidney caused by long-term administration is avoided.

The invention specifically relates to a flowerpot capable of sufficient watering. The flowerpot comprises a flowerpot body with an opening in the upper end, wherein a jacket is arranged outside the flowerpot body; a water filling hole is formed in the jacket; a water storage cavity is formed in the jacket; and a water inlet hole communicating with the water storage cavity is formed in the side wall of the flowerpot body. By means of the arranged jacket, water can enter the flowerpot body from the middle lower portion of the flowerpot body, so that sufficient irrigation of roots of plants is guaranteed, loss of soil on the surface is also avoided, and the air permeability of the flowerpot body can be greatly improved through a further arranged vent pipe.

The present invention relates to chrysanthemum nuclear factor CmNF-YB8 and its application in regulation of florescence and juvenile leaf quantity. Specifically, the present invention relates to the CmNF-YB8 gene, the open reading frame of which has the nucleotide sequence shown in the 69th to 560th positions of SEQ ID NO.1. The present invention also relates to a nuclear factor protein, which can be encoded by the CmNF-YB8 gene, or has the amino acid sequence shown in SEQ ID NO.2, or has 80%, preferably 90%, of the sequence shown in SEQ ID NO.2. %, more preferably 95, and even more preferably 99% identical and have the same biological function. The present invention also relates to the application of the CmNF-YB8 gene or the protein in the regulation of plant flowering period or the regulation of young leaf quantity. The invention can realize the convenient and effective regulation of the flowering period of the plant and the number of young leaves.

The invention provides a sesamum indicum unpollinated ovary in-vitro culture callus induction method. The method comprises the following steps: (1) material selection and disinfection: selecting a sesamum indicum plant which grows robustly and has no plant diseases and insect pests, collecting unpollinated flower buds of which anthers do not crack 1-2 days before flowering, and carrying out disinfection treatment; (2) explant pretreatment: stripping ovaries as explants, and soaking the explants in a vitamin C solution with a certain concentration for 2 hours; and (3) explant inoculation and induction culture: flatly placing and inoculating the explant on an induction culture medium, carrying out heat shock treatment for 24-48 hours, then carrying out induction culture at the culture temperature of 23-28 DEG C, carrying out dark culture for the first 30 days in the culture period, and then carrying out illumination culture. According to the method disclosed by the invention, the sesamumindicum unpollinated ovary callus is successfully induced, and the highest induction rate can reach 36.74%. The invention lays a material foundation for further establishing a complete sesamum indicum unpollinated ovary in-vitro culture system.

The invention discloses a method for increasing the weight of Feizixiao lychees per fruit. The method includes the following steps: S1, after the Feizixiao lychees have spikes in early February, manually thinning flowers, only retaining one spike at the root of each flower, and removing residual spikes; S2, covering the spikes with a plastic greenhouse, and keeping the internal temperature of thegreenhouse at 25-27 DEG C, wherein the growth and development of the spikes are promoted through temperature rise in the greenhouse, and all female flowers of the lychee spikes are promoted to flowerin late February; and S3, in the flowering stage, applying bee-assisted pollination, removing the plastic greenhouse after pollination and fertilization, and allowing fruits to grow and develop in a natural state until ripening. The method has the advantages that through a high-temperature environment in the greenhouse, development of lychee buds and early flowering are promoted; after pollinationand fertilization, the fruits further develop and ripen under a natural-temperature environment, and accordingly, the weight of the lychees per fruit is increased.

The invention provides a preparation process of coptis chinensis flower dish. The preparation process comprises following steps: step A, pre-processing: fresh coptis chinensis flowers at the scape growing and flowering stage, and impurities on the coptis chinensis flowers are removed firstly for standby application; step B, fixation: tools include a stainless steel pot, a bamboo-strip or stainlesssteel scoop, table salt and a plastic basin, the table salt is added when clear water is heated to 95 DEG C or higher, a ratio of water to the salt is 1000:4, meanwhile, the standby flowers are put in the stainless steel pot with boiling water and are heated and cooked for 5-6 min, the bamboo-strip or stainless steel scoop is utilized to stir the flowers continuously, then the flowers are taken out and placed in a stainless steel container or the plastic basin filled with cold water for first cooling; and step C, cooling: during cooling, sufficient clear water is needed, the flowers are needed to be turned in time, uniform cooling is ensured, and the flowers are subjected to secondary rinsing after about 10 min. By means of packaging, the use value of the coptis chinensis flowers can be further improved, and the prepared coptis chinensis flower dish is relatively crisp, tasty and delicious.

The invention relates to the technical field of anthurium flower planting, in particular to a retractable anthurium flower planting support. The retractable anthurium flower planting support comprisesa plurality of longitudinal containing frames symmetrically arranged at the two sides of a water distribution and temperature control box, so that water distribution and heat preservation of anthurium flowers in the containing frames at the two sides are achieved; the distance between the containing frames at the two sides and the water distribution and temperature control box can be adjusted through a contraction motor, longitudinal three-dimensional space is comprehensively utilized, and the problems that traditional anthurium flowers are subjected to spraying and temperature control from the upper portion, consequently, the longitudinal three-dimensional space is wasted and cannot be comprehensively utilized is solved; and an autorotation motor worm arranged on a connecting frame is matched with a worm wheel, so that each containing frame can autorotate along the worm wheel on the basis of fully utilizing the three-dimensional space in the longitudinal direction, and the water distribution and temperature control box can perform uniform spraying and temperature control effects on the anthurium flowers placed in the containing frames in the circumferential direction, and the problems that the anthurium flowers are placed in a three-dimensional manner, spraying is uneven, and the appearance of the anthurium flowers is affected are further solved.

The invention discloses a vacuolar processing enzyme gene RsVPE1 related to abortion of a radish flower bud. Aborted flower buds of the radish, half-aborted flower buds of the radish and normal flower buds of the radish are taken as testing materials, TDF72 of differential expressions can be obtained through cDNA (complementary deoxyribonucleic acid)-AFLP (amplified fragment length polymorphism) analysis, gDNA (genomic deoxyribonucleic acid) and cDNA of the radish vacuolar processing enzyme gene RsVPE1 are obtained through a RACE (rapid amplification of cDNA end) method, and the gDNA and the cDNA are respectively 2346bp and 1470bp; homology measurement is carried out on a cDNA sequence of the RsVPE1 gene through a BLAST (basic local alignment search tool) software, and the comparison result shows that the cDNA sequence has the highest homology with arabidopsis gamma-VPE; the expression conditions of the RsVPE1 gene in sepals, petals, stamen and pistil of normal flower buds and aborted flower buds of fertile plants and sterile plants of the aborted materials of the radish flower buds are further analyzed, the result shows that expression quantity of different parts of the aborted flower buds in the fertile plant and the sterile plant is higher than that of the corresponding parts of the normal flower buds, and the expression quantity of the sepals and the petals of the aborted flower buds is higher than that of the normal flower buds. The vacuolar processing enzyme gene RsVPE1 has an important significance in accelerating the utilization of the aborted flower bud gene in plant breeding practice of cruciferae crops.

The invention provides a method for controlling tetranychus cinnabarinus and relates to the technical field of biological control for the tetranychus cinnabarinus. According to the method, through combined action of creeping oxalis and chrysopa sinica, the tetranychus cinnabarinus of garden plants are biologically controlled. The creeping oxalis can massively trap the tetranychus cinnabarinus, andthe potted creeping oxalis which has trapped the tetranychus cinnabarinus can be further used as a natural enemy source feeding nursery of chrysopa sinica adults, cannot pollute the environment and is safe to natural enemies; the biological diversity of an ecological system is improved; as the feeding nursery of the natural enemies, the potted creeping oxalis can promote natural control for the natural enemies, so that use of insecticides is substantially reduced, the cost is saved and the benefits are increased. When the method is used for controlling the tetranychus cinnabarinus, particularly, when the flower watching stage of the creeping oxalis and the spawning period of the tetranychus cinnabarinus coincide, a best trapping effect on the tetranychus cinnabarinus on the garden plantscan be realized, and a better control effect on the tetranychus cinnabarinus can be realized.

The invention belongs to the technical field of ecological engineering, and discloses a comprehensive method for treating spartina alterniflora through fresh water submerging. Physical prevention and treatment comprises the steps of water submerging, cutting, ploughing, root smashing, and covering and shading; chemical prevention and treatment comprises the step of preparing a herbicide for spartina alterniflora treatment; biological prevention and treatment comprises the step of reducing the density of the spartina alterniflora to realize the recovery of a mud flat ecological system; and biological substitution comprises the step of according to a plant community succession rule, replacing alien invasive plants with local plants with strong competitiveness. Spartina alterniflora prevention and control research is carried out in the method, the spartina alterniflora is managed in a combined mode of fresh water submerging, ploughing and biological mulching film covering, the original ecology can be restored while expansion of the spartina alterniflora is controlled, a comprehensive spartina alterniflora prevention and control demonstration base is established in the Yellow sea mud flat, and the prevention and control mode of the spartina alterniflora is further popularized; and a wetland vegetation habitat is restored in an implementation area, the wetland ecological function is improved, waterfowl is successfully attracted for inhabitation, and thousands of gray cranes and bean geese are found in an experimental area to find food and inhabit towards the north.

The invention belongs to the technical field of biology, and discloses application of metallothionein DaMT2a and a coding gene thereof. Full-length cDNA of the metallothionein DaMT2a gene of Dioscoreaalata L. is cloned for the first time; gene expression analysis shows that the DaMT2a gene is highly expressed in male flowers and stems, expression is up-regulated under the induction of ethephon and abscisic acid, meanwhile expression of the gene is up-regulated under the induction of low temperature and mechanical injury stress, and expression is down-regulated under high temperature stress, so that the gene is possibly closely related to stress resistance of the Dioscorea alata L., and the gene can be used as an important target gene for transgenic breeding of the Dioscorea alata L.. Thegene is further transferred into a prokaryotic expression strain, and the growth of a transgenic strain under normal conditions, heavy metal ions (Cu<2+> and Cd<2+>), NaCl stress and hydrogen peroxidestress is obviously improved, so that the gene can be used as an important gene resource, and the gene can be applied to stress resistance and heavy metal stress resistance gene engineering of otherplants or microorganisms except for the Dioscorea alata L..

The invention provides a rapid propagation and in vitro preservation method of saprophytic orchids, and relates to the technical field of plant propagation. The rapid propagation and in vitro preservation method takes the fruit pods with seeds obtained after artificial pollination as objects, obtains protocorms through disinfection and aseptic germination of seeds, and induces them to obtain rhizomes, and then uses the rhizomes as The subject further established the in vitro preservation system of Dagenia, the multiplication coefficient reached 1:4 within half a year, and the in vitro preservation and subculture cycle reached about 2 years. The rapid propagation and in vitro preservation method of the present invention effectively solves the problem of rapid propagation of saprophytic orchid tissue culture, prolongs its in vitro preservation period, and is conducive to further preservation, development and utilization of saprophytic orchid germplasm resources. Lay the foundation for future resource mining.

The invention discloses a preparation method of bombax ceiba three-dimensional dried flowers. The preparation method comprises following steps: (1), pretreatment, wherein fresh bombax ceiba flowers are immersed in a color retention agent so as to maintain original color and quality of bombax ceiba flowers in subsequent drying processes, and the color retention agent is a mixture of citric acid and glycerin; (2) embedding, wherein gaps of the bombax ceiba flowers processed via step (1) are filled with degreasing cotton, the whole bombax ceiba flowers are coated with degreasing cotton, and the obtained bombax ceiba flowers are coated with granular silica gel; (3) drying, wherein vacuum freeze drying or refrigerator freeze drying are used to dry the coated bombax ceiba flowers so as to remove water in the bombax ceiba flowers; and (4), the granular silica gel and the degreasing cotton are removed so as to obtain the bombax ceiba three-dimensional dried flowers. The preparation method is used for preparing the bombax ceiba three-dimensional dried flowers; original shape, color, texture, softness, and straight shape of the bombax ceiba flowers are basically maintained; the bombax ceiba three-dimensional dried flowers can be further made into bombax ceiba three-dimensional dried flower souvenirs in forms of single flower, flowering branch, or other forms; and supply of the bombax ceiba flowers all year round becomes possible.