34 results about "Furtadoa" patented technology

The present invention relates to in planta transformation methods for plants or plant materials with a genetic construct, wherein at least one meristematic cell of a meristematic tissue of an immature inflorescence able to differentiate into a gamete of a pollen or of an ovule is exposed and then transformed, wherein the transformation can be performed to yield a stable integration or to yield a transient introduction of a genetic construct of interest. Further provided are methods for manufacturing a transgenic plant, or methods for manufacturing a genetically manipulated plant based on the in planta transformation methods according to the present invention. In addition, there is provided a plant or a progeny thereof manufactured according to the transformation and/or manufacturing methods according to the present invention.

The invention relates to the method for identifying the varieties of lilium brownii by adopting an SSR (simple sequence repeat) molecular marking technology. A result shows that: two pairs of high-polymorphism and high-repeatability primers are screened from 13 pairs of primers for detecting 171 fragments comprising 131 polymorphic fragments, 4 to 22 fragments can be detected by each pair of primers with the sizes of 100 to 250bp, 1.69 bands are amplified by each pair of primers, and the polymorphism rate is 76.70 percent; NTSYS-pc2.11 is used for similarity coefficient and cluster analysis, the genetic similarity coefficient is 0.5882 to 09412, and the average similarity coefficient is 0.7647; materials are divided into six major classes by cluster analysis, the genetic differences between 11 lilium brownii germplasms are irrelevant to varietal characteristics, flower forms, the colors of filaments and styluses, but have certain correlation with the colors of petals, flower genes can better reflect genetic relationships between the lilium brownii germplasms, different varieties have different colors, and the primers can be designed by controlling related genes of flowers to provide an important means for further distinguishing the genetic relationships.

The invention discloses a castanopsis hystrix pollen collecting and storing method for increasing the germination rate and improving the pollen viability. The method comprises the following steps that when male parent male flowers are in a transitional period of an early blooming period and a full blooming period, male parent male flower inflorescence is collected at 9:00-11:00 or 15:00-17:00; the male flower inflorescence is dried for 12-24 h in a sterile room at the temperature of 22 DEG C-28 DEG C under illumination of 80 W-160 W, when a large amount of anther blows out and a large amount of yellow pollen scatters during gentle shaking, drying is completed; the dried male flower inflorescence is screened with a pollen sieve, pollen is collected and contained in a container, an opening of the container is sealed, and the container is placed in an environment at the temperature of minue 60 DEG C-minus 100 DEG C or liquid nitrogen to be stored. Furthermore, when the pollen needs to be germinated, the stored pollen is placed in a liquid culture medium and can be germinated at the temperature of 25 DEG C-35 DEG C. Accordingly, a castanopsis hystrix pollen collecting, drying, storing and viability detecting system is formed for the first time, the method is simple, rapid and high in operability, the castanopsis hystrix pollen viability can be improved, and the manual cross pollination success rate of castanopsis hystrix can be increased.

The invention relates to plant genes and particularly discloses a novel symmetry gene CmCYC2c of chrysanthemum morifolium and a novel application of the symmetry gene CmCYC2c in the aspect of regulation of ligulate flower petal traits of the chrysanthemum morifolium, especially, in the aspects of increase of the number of ligulate flower petals and increase of petal length. The nucleotide sequence of the symmetry gene CmCYC2c is represented as SEQ ID NO.1. Based on the gene and the application thereof, the CmCYC2c gene is integrated to a chamomile genome with a transgenic technology, the flower type is primarily and directionally improved, so that the number of the ligulate flowers is increased, the petal length is increased, and a novel and practical method is provided for primary selection of new species of chrysanthemum morifolium varieties with the genetic engineering technology.

The invention relates to an interspecific cross breeding method of fagopyrum dibotrys and tartary buckwheat and application. According to the interspecific cross breeding method of the fagopyrum dibotrys and the tartary buckwheat and application, with tetraploid tartary buckwheat as female parents and a diploid fagopyrum dibotrys complex plant doubled line and/or tetraploid fagopyrum dibotrys which naturally exists as male parents, crossing is conducted, and a cross breed obtained by adopting the interspecific cross breeding method of the fagopyrum dibotrys and the tartary buckwheat can live through the winter in warm southern areas, so that years of asexual propagating expanding production and cultivation are achieved. According to the breeding method, not only is the interspecific heterosis of the fagopyrum dibotrys and the tartary buckwheat used, but also the heterosis among different fagopyrum dibotrys lines is used, the perennial characteristic and the multiple regeneration capability of the interspecific cross breed fagopyrum dibotrys are further improved, and thus the regeneration capability of branch cutting and multiple cutting of cross breed plants is strong enough to support overwintering and propagating expanding of multiple branch cutting, so that the heterosis of the cross breed is maintained permanently, and the cross breed can be widely applied and popularized in production.

The invention relates to the field of rumen regulation and control, in particular to a natural product preparation for promoting the rumen fermentation. The natural product preparation is prepared through the following steps: after grinding homalomena occulta roots, extracting with 60-80 mass percent of ethanol solution, and performing nitrogen flushing and freeze drying to obtain dry powder. Through researching, the inventor has discovered that the homalomena occulta roots have a certain effect on promoting the rumen fermentation. Homalomena occulta has a wide source property and cooperativity, the experiment through adopting a filter paper flat band method shows that obscured homalomena rhizome volatile oil has a function of inhabiting bacterium burgeri on flocks and herds. Therefore, the homalomena occulta root extractive preparation is hopeful to further comprehensively improving the rumen function and promoting the health of ruminants after scientific compatibility.

The invention relates to the field of traditional Chinese medicine external application agents, and particularly discloses an external application agent for treating pelvic inflammation of cold uterustype and a preparation method thereof. The external application agent for treating the pelvic inflammation of the cold uterus type is endowed with the effects of relieving pain and diminishing inflammation in virtue of mutual compatibility of radix angelicae dahuricae, radix dipsaci asperoidis, zanthoxylum schinifolium, ligusticum chuanxiong, cortex acanthopanacis, homalomena rhizome and trberculate speranskia herb, so that the external application agent is capable of effectively diminishing inflammation and removing swelling; the external application agent is endowed with the effects of dispelling wind and eliminating dampness in virtue of mutual compatibility of the zanthoxylum schinifolium, the homalomena rhizome, cortex illicii, Chinese viscum herb, argy wormwood leaves, the trberculate speranskia herb, incised notopterygium rhizome and roots, double-teeth pubescent angelica roots and the ligusticum chuanxiong, so that the external application agent is capable of effectively removing coldness and dampness from the uterus of the patient; and the external application agent is also endowed with the effects of promoting blood circulation for regulating menstruation in virtue of mutual compatibility of the argy wormwood leaves, the trberculate speranskia herb, radix paeoniae rubra, Chinese angelica roots, frankincense, myrrh, common buried rubber, zedoray rhizome and red-rootedsalvia roots, so that the external application agent is capable of effectively promoting blood circulation for regulating menstruation of the patient. Moreover, in virtue of the effects of an absorption enhancer, a ginger brown-sugar preparation and a scallion stalk extract, the external application agent becomes further capable of preventing and treating bacteria in the uterus of the patient.

The invention relates to a medicine composition with a special effect of tonifying yang. The medicine composition is prepared from the following components in parts by mass: 110-130 parts of radix paeoniae alba, 50-70 parts of radix astragali seu hedysari, 40-50 parts of rhizoma drynariae, 40-50 parts of caulis spatholobi, 20-30 parts of herba epimedii, 20-30 parts of radix morindae officinalis, 20-30 parts of radix clematidis, 20-30 parts of herba lycopodii, 20-30 parts of herba speranskiae tuberculatae, 20-30 parts of radix angelicae sinensis, 20-30 parts of rhizoma homalomenae, 15-25 parts of radix glycyrrhizae preparata and 25-35 parts of centipede. According to the medicine composition, radix paeoniae alba, radix astragali, rhizoma drynariae, caulis spatholobi, herba epimedii, radix morindae officinalis and radix clematidis are taken as main medicines, and the seven main medicines are synergistically matched, so that the symptoms such as lumbago, lumbar muscle strain, cramp of legs and feet, flaccid paralysis, rheumatic arthralgia and tendon and vessel spasm are effectively improved, and a yang-tonifying medicinal effect is provided to improve various yang deficiency symptoms; meanwhile, the lycopodium clavatum, the garden balsam stem, the angelica sinensis and the obscured homalomena rhizome are used as ministerial drugs, so that the effects of inducing diuresis, detoxifying, stimulating the menstrual flow, penetrating the stem, enriching and promoting blood circulation and the like are achieved; and finally, honey-fried licorice root and centipede are used as adjuvants, and the detoxification function of the adjuvants is utilized, so that damage to liver and kidney caused by long-term administration is avoided.

The invention specifically relates to a flowerpot capable of sufficient watering. The flowerpot comprises a flowerpot body with an opening in the upper end, wherein a jacket is arranged outside the flowerpot body; a water filling hole is formed in the jacket; a water storage cavity is formed in the jacket; and a water inlet hole communicating with the water storage cavity is formed in the side wall of the flowerpot body. By means of the arranged jacket, water can enter the flowerpot body from the middle lower portion of the flowerpot body, so that sufficient irrigation of roots of plants is guaranteed, loss of soil on the surface is also avoided, and the air permeability of the flowerpot body can be greatly improved through a further arranged vent pipe.

The invention discloses a method for increasing the weight of Feizixiao lychees per fruit. The method includes the following steps: S1, after the Feizixiao lychees have spikes in early February, manually thinning flowers, only retaining one spike at the root of each flower, and removing residual spikes; S2, covering the spikes with a plastic greenhouse, and keeping the internal temperature of thegreenhouse at 25-27 DEG C, wherein the growth and development of the spikes are promoted through temperature rise in the greenhouse, and all female flowers of the lychee spikes are promoted to flowerin late February; and S3, in the flowering stage, applying bee-assisted pollination, removing the plastic greenhouse after pollination and fertilization, and allowing fruits to grow and develop in a natural state until ripening. The method has the advantages that through a high-temperature environment in the greenhouse, development of lychee buds and early flowering are promoted; after pollinationand fertilization, the fruits further develop and ripen under a natural-temperature environment, and accordingly, the weight of the lychees per fruit is increased.

The invention provides a preparation process of coptis chinensis flower dish. The preparation process comprises following steps: step A, pre-processing: fresh coptis chinensis flowers at the scape growing and flowering stage, and impurities on the coptis chinensis flowers are removed firstly for standby application; step B, fixation: tools include a stainless steel pot, a bamboo-strip or stainlesssteel scoop, table salt and a plastic basin, the table salt is added when clear water is heated to 95 DEG C or higher, a ratio of water to the salt is 1000:4, meanwhile, the standby flowers are put in the stainless steel pot with boiling water and are heated and cooked for 5-6 min, the bamboo-strip or stainless steel scoop is utilized to stir the flowers continuously, then the flowers are taken out and placed in a stainless steel container or the plastic basin filled with cold water for first cooling; and step C, cooling: during cooling, sufficient clear water is needed, the flowers are needed to be turned in time, uniform cooling is ensured, and the flowers are subjected to secondary rinsing after about 10 min. By means of packaging, the use value of the coptis chinensis flowers can be further improved, and the prepared coptis chinensis flower dish is relatively crisp, tasty and delicious.

The invention belongs to the technical field of biology, and discloses application of metallothionein DaMT2a and a coding gene thereof. Full-length cDNA of the metallothionein DaMT2a gene of Dioscoreaalata L. is cloned for the first time; gene expression analysis shows that the DaMT2a gene is highly expressed in male flowers and stems, expression is up-regulated under the induction of ethephon and abscisic acid, meanwhile expression of the gene is up-regulated under the induction of low temperature and mechanical injury stress, and expression is down-regulated under high temperature stress, so that the gene is possibly closely related to stress resistance of the Dioscorea alata L., and the gene can be used as an important target gene for transgenic breeding of the Dioscorea alata L.. Thegene is further transferred into a prokaryotic expression strain, and the growth of a transgenic strain under normal conditions, heavy metal ions (Cu<2+> and Cd<2+>), NaCl stress and hydrogen peroxidestress is obviously improved, so that the gene can be used as an important gene resource, and the gene can be applied to stress resistance and heavy metal stress resistance gene engineering of otherplants or microorganisms except for the Dioscorea alata L..