Vacuolar processing enzyme gene RsVPE1 related to abortion of radish flower bud

An enzyme gene, radish technology, applied in the field of vacuolar processing enzyme gene RsVPE1, can solve the problem of unclear death mechanism of abortion

Inactive Publication Date: 2013-10-30
NORTHWEST A & F UNIV
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Problems solved by technology

At present, there are few research reports on plant bud failure, and the mechanism of its abortion death is even less clear

Method used

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  • Vacuolar processing enzyme gene RsVPE1 related to abortion of radish flower bud
  • Vacuolar processing enzyme gene RsVPE1 related to abortion of radish flower bud
  • Vacuolar processing enzyme gene RsVPE1 related to abortion of radish flower bud

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Embodiment Construction

[0079] The applicant used cDNA-AFLP technology to select the radish cytoplasmic male sterile line B-T18 provided by the Cabbage Research Laboratory of the College of Horticulture, Northwest A&F University (observation of histocytological characteristics during the process of radish flower bud abortion, Northwest Agricultural Journal, 2008, 17 (5): 272-276) The germinated seeds of the test cross first generation 09L9 (hereinafter referred to as the radish aborted material 09L9) were sown in the greenhouse after low-temperature vernalization, and the aborted seeds that stopped growing and started to turn yellow were taken from the same plant during the flowering period The gene expression profiles of flower buds, aborted transitional flower buds and normal flower buds were analyzed, and 54 TDFs were obtained, among which the size of TDF72 amplified by primer A7T19 was 189bp similar to the GAMMA-VPE of Arabidopsis lyrata subsp.lyrata, in The expression in "bud abortion → transitio...

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Abstract

The invention discloses a vacuolar processing enzyme gene RsVPE1 related to abortion of a radish flower bud. Aborted flower buds of the radish, half-aborted flower buds of the radish and normal flower buds of the radish are taken as testing materials, TDF72 of differential expressions can be obtained through cDNA (complementary deoxyribonucleic acid)-AFLP (amplified fragment length polymorphism) analysis, gDNA (genomic deoxyribonucleic acid) and cDNA of the radish vacuolar processing enzyme gene RsVPE1 are obtained through a RACE (rapid amplification of cDNA end) method, and the gDNA and the cDNA are respectively 2346bp and 1470bp; homology measurement is carried out on a cDNA sequence of the RsVPE1 gene through a BLAST (basic local alignment search tool) software, and the comparison result shows that the cDNA sequence has the highest homology with arabidopsis gamma-VPE; the expression conditions of the RsVPE1 gene in sepals, petals, stamen and pistil of normal flower buds and aborted flower buds of fertile plants and sterile plants of the aborted materials of the radish flower buds are further analyzed, the result shows that expression quantity of different parts of the aborted flower buds in the fertile plant and the sterile plant is higher than that of the corresponding parts of the normal flower buds, and the expression quantity of the sepals and the petals of the aborted flower buds is higher than that of the normal flower buds. The vacuolar processing enzyme gene RsVPE1 has an important significance in accelerating the utilization of the aborted flower bud gene in plant breeding practice of cruciferae crops.

Description

technical field [0001] The invention relates to the field of genes in molecular biology, in particular to a vacuolar processing enzyme gene RsVPE1 related to radish flower bud abortion. Background technique [0002] Radish (Raphanus sativus L.) is an important vegetable crop native to China. It was called "radish" in ancient times. Radish is rich in nutrition, suitable for both medicine and food, and is favored by people. The planting area of ​​radish in my country is about 1.2 million hectares, ranking the top three in the planting area of ​​various vegetables. [0003] Radish is an annual herbaceous plant belonging to the genus Radish of the Brassicaceae family. It generally begins to differentiate flower buds after vernalization. Under normal growth conditions, it completes a life cycle through normal budding, flowering and fruiting. In many years of breeding practice, bud failure often occurs in Chinese cabbage, radish and other plants, that is, flower bud abortion, th...

Claims

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Application Information

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IPC IPC(8): C12N15/57
Inventor 张鲁刚张静
Owner NORTHWEST A & F UNIV
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