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Chrysanthemum nuclear factor cmnf-yb8 and its application in flowering regulation and juvenile leaf number regulation

A cmnf-yb8, 1.cmnf-yb8 technology, applied in the field of molecular biology, can solve problems such as unclear mechanism of chrysanthemum transition

Active Publication Date: 2021-08-03
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mechanism of the transition from juvenile to adulthood in chrysanthemums is unclear

Method used

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  • Chrysanthemum nuclear factor cmnf-yb8 and its application in flowering regulation and juvenile leaf number regulation
  • Chrysanthemum nuclear factor cmnf-yb8 and its application in flowering regulation and juvenile leaf number regulation
  • Chrysanthemum nuclear factor cmnf-yb8 and its application in flowering regulation and juvenile leaf number regulation

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Experimental program
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Embodiment

[0029] Materials and Methods

[0030] Plant Material and Handling

[0031] The experimental material in this study is the green chrysanthemum variety 'Qiuyan' (Chrysanthemum morifolium cv. FallColor). Chrysanthemum plants are multiplied by tissue culture. They were then transplanted into 9 cm diameter pots containing a 1:1 (v / v) mixture of peat and vermiculite in a long-day (16 h light / 8 h dark) cultivation room at 23 ± 1 °C, 40% relative humidity and 100 μmol m -2 the s -1 Cultivate under light intensity.

[0032] For the short-day treatment, plants grown 5 days after transplantation were transferred to an incubation room and kept under short-day (8 hr light / 16 hr dark) conditions. For low temperature treatment, the plants were transferred to a culture room at 4° C. and cultured for 30 days under long-day light (16 hours of light / 8 hours of darkness). GA treatment, the plants 5 days after transplantation were treated with 100 μM GA 4 / 7 (Sigma) treated and maintained ...

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Abstract

The present invention relates to chrysanthemum nuclear factor CmNF-YB8 and its application in regulation of florescence and juvenile leaf quantity. Specifically, the present invention relates to the CmNF-YB8 gene, the open reading frame of which has the nucleotide sequence shown in the 69th to 560th positions of SEQ ID NO.1. The present invention also relates to a nuclear factor protein, which can be encoded by the CmNF-YB8 gene, or has the amino acid sequence shown in SEQ ID NO.2, or has 80%, preferably 90%, of the sequence shown in SEQ ID NO.2. %, more preferably 95, and even more preferably 99% identical and have the same biological function. The present invention also relates to the application of the CmNF-YB8 gene or the protein in the regulation of plant flowering period or the regulation of young leaf quantity. The invention can realize the convenient and effective regulation of the flowering period of the plant and the number of young leaves.

Description

technical field [0001] The invention relates to the field of molecular biology technology, in particular to chrysanthemum nuclear factor CmNF-YB8 and its application in flowering regulation and young leaf quantity regulation. Background technique [0002] Flowering at the appropriate time is key to successful sexual reproduction in plants and is determined by a complex interplay of environmental condition signals and internal signals. Angiosperms have developed several mechanisms to coordinate the control of floral transformation, including the photoperiod pathway, vernalization pathway, gibberellin pathway, and age pathway. These flowering pathway signals eventually converge on a set of downstream flowering integrators, including MADS-box genes, FLOWERING LOCUS T (FT), SUPPRESSOR OFOVER EXPRESSION OF CO1 (SOC1), APETALA1 (AP1), and a plant-specific transcription factor, LEAFY( LFY). [0003] Among these flowering pathways, the age pathway provides an endogenous developmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/02A01H5/12A01H6/20A01H6/14
CPCC07K14/415C12N15/8203C12N15/8261C12N15/827
Inventor 洪波高俊平马超徐彦杰
Owner CHINA AGRI UNIV
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