Chrysanthemum nuclear factor CmNF-YB8 and application thereof in regulation of flowering time and regulation of quantity of young leaves

A technology of nuclear factor protein and quantity, which is applied in the fields of application, genetic engineering, and plant gene improvement, and can solve problems such as the unclear transition mechanism of chrysanthemums

Active Publication Date: 2017-11-03
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the mechanism of the transition from juvenile to adulthood in chrysanthemums is unclear

Method used

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  • Chrysanthemum nuclear factor CmNF-YB8 and application thereof in regulation of flowering time and regulation of quantity of young leaves
  • Chrysanthemum nuclear factor CmNF-YB8 and application thereof in regulation of flowering time and regulation of quantity of young leaves
  • Chrysanthemum nuclear factor CmNF-YB8 and application thereof in regulation of flowering time and regulation of quantity of young leaves

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Embodiment

[0029] Materials and Methods

[0030] Plant Material and Handling

[0031] The experimental material in this study is the green chrysanthemum variety 'Qiuyan' (Chrysanthemum morifolium cv. FallColor). Chrysanthemum plants are multiplied by tissue culture. They were then transplanted into 9 cm diameter pots containing a 1:1 (v / v) mixture of peat and vermiculite in a long-day (16 h light / 8 h dark) cultivation room at 23 ± 1 °C, 40% relative humidity and 100 μmol m -2 the s -1 Cultivate under light intensity.

[0032] For the short-day treatment, plants grown 5 days after transplantation were transferred to an incubation room and kept under short-day (8 hr light / 16 hr dark) conditions. For low temperature treatment, the plants were transferred to a culture room at 4° C. and cultured for 30 days under long-day light (16 hours of light / 8 hours of darkness). GA treatment, the plants 5 days after transplantation were treated with 100 μM GA 4 / 7 (Sigma) treated and maintained ...

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Abstract

The invention relates to chrysanthemum nuclear factor CmNF-YB8 and application thereof in regulation of flowering time and regulation of quantity of young leaves, and specifically relates to a CmNF-YB8 gene. The open reading frame of the gene has the nucleotide sequences shown as the 69th to 560th positions in SEQ ID NO.1. The invention also relates to a nuclear factor protein which can be coded through the CmNF-YB8 gene or which has the amino acid sequence shown as SEQ ID NO.2 or has the identity which is up to 80%, preferably 90%, more preferably 95 and further preferably 99% relative to the sequence as shown in SEQ ID NO.2 and has the protein with the same biological function. The invention also relates to application of the CmNF-YB8 gene or the protein in the regulation of flowering time and the regulation of the quantity of the young leaves of a plant. With the adoption of the CmNF-YB8 gene, the flowering time and the quantity of the young leaves of the plant can be conveniently and effectively regulated.

Description

technical field [0001] The invention relates to the field of molecular biology technology, in particular to chrysanthemum nuclear factor CmNF-YB8 and its application in flowering regulation and young leaf quantity regulation. Background technique [0002] Flowering at the appropriate time is key to successful sexual reproduction in plants and is determined by a complex interplay of environmental condition signals and internal signals. Angiosperms have developed several mechanisms to coordinate the control of floral transformation, including the photoperiod pathway, vernalization pathway, gibberellin pathway, and age pathway. These flowering pathway signals eventually converge on a set of downstream flowering integrators, including MADS-box genes, FLOWERING LOCUS T (FT), SUPPRESSOR OFOVER EXPRESSION OF CO1 (SOC1), APETALA1 (AP1), and a plant-specific transcription factor, LEAFY( LFY). [0003] Among these flowering pathways, the age pathway provides an endogenous developmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00
CPCC07K14/415C12N15/8203C12N15/8261C12N15/827
Inventor 洪波高俊平马超徐彦杰
Owner CHINA AGRI UNIV
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