A kind of rapid propagation and in vitro preservation method of saprophytic orchid

A technology of in vitro preservation and orchids, which is applied in the direction of plant preservation, botanical equipment and methods, gardening methods, etc., can solve the problems that the artificial cultivation of saprophytic orchids has not been successful, and achieve the effect of prolonging the in vitro preservation period

Active Publication Date: 2022-02-18
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

At present, among the saprophytic species of Orchidaceae, only Gastrodia elata has been artificially propagated and created great value, and the artificial cultivation of other saprophytic orchids has not yet been successful.

Method used

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  • A kind of rapid propagation and in vitro preservation method of saprophytic orchid
  • A kind of rapid propagation and in vitro preservation method of saprophytic orchid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Obtaining of explants

[0029] The vigorous flowering plants of Cymbidium macrorhizon were selected in the wild for artificial cross-pollination. The flowering period of the daikon orchid in Kunming is around mid-July. Flowers that have fully unfolded for 1 to 2 days are selected for pollination. Use a sterilized bamboo toothpick to pick pollen grains and stick them on the stigma of another flower, and then mark them. About 180 days after pollination, the fruit pods of the orchid daikon turned light yellow-green but did not crack, and contained a large number of fully developed seeds. The fruit pods at this period were used as explants for subsequent experiments.

[0030] 2. Disinfection and germination of explants

[0031] Scrub the surface of uncracked geranium fruit pods with clean gauze, move them into the ultra-clean workbench, sterilize with 75% alcohol (volume fraction) for 1 minute, and then use 0.2% (mass fraction) HgCl 2 Soak the solution for 10 minutes, ...

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Abstract

The invention provides a rapid propagation and in vitro preservation method of saprophytic orchids, and relates to the technical field of plant propagation. The rapid propagation and in vitro preservation method takes the fruit pods with seeds obtained after artificial pollination as objects, obtains protocorms through disinfection and aseptic germination of seeds, and induces them to obtain rhizomes, and then uses the rhizomes as The subject further established the in vitro preservation system of Dagenia, the multiplication coefficient reached 1:4 within half a year, and the in vitro preservation and subculture cycle reached about 2 years. The rapid propagation and in vitro preservation method of the present invention effectively solves the problem of rapid propagation of saprophytic orchid tissue culture, prolongs its in vitro preservation period, and is conducive to further preservation, development and utilization of saprophytic orchid germplasm resources. Lay the foundation for future resource mining.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a rapid propagation and in vitro preservation method of saprophytic orchids. Background technique [0002] Cymbidium macrorhizon is a plant of the genus Orchidaceae, without green leaves. It is the only saprophytic species in this genus. It is distributed in the understory or forest edge at an altitude of 400-2100 meters in Sichuan, Yunnan, Guizhou and Guangxi in my country. Usually, the seeds of orchid plants are very small, about 0.05-6.0mm long and 0.01-0.9mm wide, and the number of seeds contained in each fruit varies from thousands to millions, but because the seeds do not contain endosperm, The germination rate is very low under natural conditions. Orchidaceae species are traditionally propagated by ramets, but the efficiency is not high. Because saprophytic orchids need to rely on symbiosis with fungi to obtain nutrients for growth, division propaga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H4/00A01N3/00
CPCA01H1/02A01H4/008A01N3/00
Inventor 何俊浦秀丽李村富杨俊波
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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