A method for tissue culture rapid propagation and in vitro preservation of chicken bone Changshan seedlings

A technology of in vitro preservation and tissue culture rapid propagation, which is applied in the direction of plant preservation, botanical equipment and methods, horticultural methods, etc., can solve the problems of inability to meet large-scale seedling production and low reproduction efficiency, and achieve the goal of being conducive to preservation And the effect of development and utilization, prolonging the time of in vitro storage, and prolonging the period of in vitro storage

Active Publication Date: 2021-11-19
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, Jigu Changshan is mostly propagated by seeds, divisions or cuttings, and the reproduction efficiency is low, which cannot meet the requirements of large-scale seedling production.

Method used

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  • A method for tissue culture rapid propagation and in vitro preservation of chicken bone Changshan seedlings
  • A method for tissue culture rapid propagation and in vitro preservation of chicken bone Changshan seedlings
  • A method for tissue culture rapid propagation and in vitro preservation of chicken bone Changshan seedlings

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Experimental program
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Effect test

Embodiment 1

[0030] 1. Disinfection of explants

[0031] Take the newly extracted lateral bud stem section of a strong single plant of chicken bone Changshan as explant, soak it in 1% soapy water for 10 minutes, then wash it with tap water for 1 hour, put it on the ultra-clean table, cut it into a stem section with a single bud, and use 75 % alcohol for 10 seconds, washed with sterile water for 3 times, disinfected with 0.1% mercuric solution for 3 to 8 minutes, washed with sterile water for 3 to 5 times, and inoculated on basic culture MS without adding any hormones. For cross-contamination, place 1 stem segment with buds in each bottle. All culture media were added with 20g / L sucrose, solidified with 5.6g / L agar (the same reasoning below), adjusted the pH value to 5.8, cultured at 25±3°C, and light intensity of 20-40μmol / (m 2 s), light 12h / d. The stems with buds were inserted into different first-generation culture media and observed every day. After 3 days, pollution appeared successi...

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Abstract

The invention provides a method for tissue culture rapid propagation and in vitro preservation of chicken bone Changshan seedlings, and relates to the technical field of germplasm preservation. The present invention takes the budded stem section of Jigu Changshan as the object, obtains adventitious buds through explant disinfection, primary culture, and performs proliferation induction, rooting and in vitro preservation on it, and establishes tissue culture and rapid propagation of Jigu Changshan And the in vitro preservation system, the monthly multiplication coefficient reaches 1:4, and the in vitro preservation subculture cycle reaches about 1 year. Utilizing the tissue culture rapid propagation and in vitro preservation method described in the present invention prolongs the time for in vitro preservation, can reduce the workload of transplanting tissue culture seedlings, and also avoids the possibility of genetic changes caused by successive generations. Conservation and sustainable use are guaranteed.

Description

technical field [0001] The invention belongs to the technical field of germplasm preservation, and in particular relates to a method for tissue culture rapid propagation and in vitro preservation of chicken bone Changshan seedlings. Background technique [0002] Alstonia yunnanensis (Alstonia yunnanensis) is a plant of the genus Alstonia yunnanensis in the Apocynaceae family. It is an endemic species in my country and is distributed in hillsides or valleys at an altitude of 1100-2400 meters in southwest China. Both its roots and leaves are used for medicine, and the monoterpene indole alkaloid contained in the root has the effect of lowering blood pressure, so it is an important wild plant germplasm resource for the development of natural blood pressure lowering drugs; Fragrant, saucer-shaped flowers clustered on the top of the branches, which is of great ornamental value; the plant type is an upright shrub about 2 meters high, with many branches, and the branches are resist...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01N3/00
CPCA01H4/008A01N3/00
Inventor 李春芳何俊杨俊波
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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