107 results about "Fucus evanescens" patented technology

The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus 5 containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.

The invention provides a method for preparing fucoidan, relating to the field of production and application of food additives. The method comprises the following steps of: adding distilled water according to a material to liquid ratio of 1:40-4:80 g / mL with brown algae, such as kelps, undaria pinnatifida, and the like as raw materials; treating by a colloid mill and then carrying out microwave-assisted extraction or ultrasonic wave-assisted extraction, wherein, in the microwave-assisted extraction process, the microwave time lasts 20-40min, the microwave extraction temperature is 60-80 DEG C, and the microwave power is 400-500W; and in the ultrasonic wave-assisted extraction process, the ultrasonic power is 800-100W, the microwave time lasts 20-40min, and the extraction temperature is 60-90 DEG C; and centrifuging an extracting solution, adding a proper amount of 2% CaCl2 solution into a supernatant, stirring, keeping constant temperature for 20-30h at 35-40 DEG C, centrifuging, adding 50-100% ethanol into the supernatant until the ethanol concentration of the solution is 15-20%, stirring, filtering, concentrating, adding 90-100% ethanol into a concentrated solution until the ethanol concentration of the solution is 55-60%, centrifuging again and drying a precipitate in vacuum to obtain white or yellow-white fucoidan powder.

The invention provides a method for preparing algae fucosan and fucoxanthin in an enzymic way. The method specifically comprises the following steps of: preparing brown algae dry powder; processing the brown algae dry powder by enzymolysis: uniformly mixing the brown algae dry powder with a complex enzyme preparation, then adding deionized water to perform enzymolysis; performing acid treatment; acquiring concentrated liquor; and acquiring fucosan: acquiring crude fucoxanthin, and then acquiring pure fucoxanthin. The complex enzyme is composed of pectinase, hemicellulase and cellulose. The brown algae fucosan and the fucoxanthin prepared by the method are high in yield. The method is simple in operation, environment-friendly, efficient, and suitable for being used for synchronously extracting high-purity fucosan and fucoxanthin from brown algae with massive operation.

The invention discloses a comprehensive utilization usage of seaweed; especially production method of involving producing seaweed agricultural product and fucosido- polysaccharide; which comprise the following parts: adopting marine biology sodium as main material; applying freezing disintegrating technology, ultrasonic wave water and lixivium extraction steps; extracting inherent autogenous plant growing modifier in maximum limit and keeping biological activity; separating fucosido-polysaccharide and producing bottom of last leaving to fertilizer, soil modifier and fodder; that can be using fully.

The invention provides a culture method for increasing the yield of fucoxanthin contained in diatom. The culture method comprises the following steps of: (1) heterotrophically culturing activated diatom for 3-8 days so that the diatom is in a logarithmic growth period; (2) inoculating a diatom culture solution obtained from the step (1) in the logarithmic growth period as a seed solution into a heterotrophic culture vessel which contains a heterotrophic culture medium (culture medium I) according to 5%-50% volume of inoculation amount to carry out heterotrophic culture for 4-12 days at the temperature of 20-34 DEG C, wherein tomato extractives are added to the heterotrophic culture medium (culture medium I) obtained from the step (2). The culture method provided by the invention can enhance the biomass concentration of the diatom and increase the fucoxanthin content of a stable diatom body, thereby increasing the yield of the fucoxanthin contained in the diatom.

The present invention provides process of utilizing kelp comprehensively and extracting fucoidin. The process has secondary soaking of kelp to raise the leaching rate of iodine, mannitol and fucoidin and to lower algin extracting cost. The water after enhanced stirring is flocculated to purify, and this can improve the iodine and mannitol extracting condition for high product yield and simplify post purification of fucoidin. The process of the present invention has improved product extracting condition, lowered production cost, obviously raised iodine, mannitol and fucoidin extracting rate and raised kelp resource utilizing value.

The invention provides compositions and methods for engineering E. coli or other host production bacterial strains to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.

The invention relates to an extracting and purifying method of fucoidan from fucus vesiculosus. The extracting and purifying method comprises the following steps of: S1, with sea-tangle waste generated after mannitol is extracted as raw material, adding deionized water with pH of 5-6 according to a proportion, intermittently extracting for 3-6 times under the microwave condition of 2450MHz and 600-1200W, wherein each extraction is carried out for 2-7min and a certain proportion of deionized water is replenished every time; S2, centrifuging an extraction solution to obtain supernate, concentrating the supernate and then adding a certain proportion of alcohol, generating a sediment, centrifuging again to obtain supernate; S3, cooling the supernate to 5-10DEG C, slowly adding a certain proportion of alcohol, seriously stirring, gradually generating a white particle sediment; and S4, collecting the sediments, washing by using absolute ethyl alcohol, and drying to obtain the fine particle fucoidan from fucus vesiculosus.

The invention discloses a fucoidan-galactosan sulfate with a novel structure. Fucoidan-galactosan sulfate provided by the present invention is a sulfated polysaccharide composed of fucose and galactose. According to the chemical constitution of the sugar chain, a fundamental chain is formed by -4-alpha-L-Fuc-(1,3)-alpha-L-Fuc-(1,3)-alpha-L-Fuc-(1,3)-alpha-L-Fuc-(1-residue. Every 4 sugar residues comprise a branched chain, which is composed of alpha-L-Fuc(1- or beta-D-Gla-(1,6)-beta-D-Gla-(1-. Sulfated group is connected to the 2 or 4 site of fucose, or on the 3 or 4 site of galactose. Meanwhile, the invention discloses a preparation method and an application of fucoidan-galactosan sulfate. Fucoidan-galactosan sulfate provided by the present invention can be exploited into medicines for treating kidney diseases.

The invention provides a method for preparing sea cucumber fucoidan and sea cucumber glycoprotein. According to the method, acaudina molpadioides serves as the raw material, a mild stepped enzymolysis processing method is mainly adopted, an ultrafiltration membrane is utilized to separate out a mixture of crude polysaccharide and glycoprotein, then separation, purification and preparation of the sea cucumber fucoidan and the sea cucumber glycoprotein are achieved through a Q-Sepharose-F-F ion exchange column and an Sephadex G-150 sephadex column, a prepared sea cucumber fucoidan product is fawn, and the sea cucumber glycoprotein is faint yellow. According to the method, a co-production method is adopted, main ingredients such as polysaccharide and glycoprotein in the acaudina molpadioides can be prepared in a jointed mode, emission of waste is reduced in the extraction process, and production cost is lowered.

The invention discloses a method for promoting accumulation of fucoxanthin and / or lipids in phaeodactylum tricornutum. The method is characterized by including the following steps: inoculating a seawater culture solution with a plurality of phaeodactylum tricornutum in a growing period according to the volume ratio of 10%, adding 2, 4-epibrassinolide into the seawater culture solution to enable the final concentration of the seawater culture solution to be 0.05-0.15mg / L, fully shaking a flask three times a day at the culture temperature of 20 DEG C under the conditions of illumination intensity being 5000lx and photoperiod being 12h / 12h, and culturing 10d of the phaeodactylum tricornutum, wherein the optimal concentration for fucoxanthin accumulation is 0.05mg / L, and the optimal concentration for lipid accumulation is 0.1mg / L. The method has the advantages that through addition of a certain amount of 2, 4-epibrassinolide in a culture medium, the accumulation speed of the fucoxanthin and / or the lipids in the phaeodactylum tricornutum can be increased effectively, and the content of the fucoxanthin and / or the lipids in the phaeodactylum tricornutum can be increased remarkably; the method is simple to operate and low in cost, and production cycle is short.

The invention relates to a new application of fucoidan sulfate in preparation of drug for preventing and treating chronic obstructive pulmonary disease (COPD). The fucoidan sulfate has fucose content greater than 29% and sulfate group content greater than 30%; and is a polysaccharide sulfate extracted from Phaeophyta algae such as Zostera Marina L., Laminaria Japonica Aresch., Sargassum Phyllocystum, Surgassum Thunbergii, Sargassum Integerrimum, Sargassum Fusiforme (Harv.) Setchel, Sargassum naozhouense, Sargassum Muticum, Sargassum Pallidum, etc. Animal tests show that fucoidan sulfate has definite curative effects in preventing and treating COPD, and relieving development of COPD.

The invention discloses a pretreatment method of brown alga samples for fucoaxanthin extraction. The pretreatment method is characterized by concretely including the steps that step1, the fresh brown algae are frozen for 1-30 days at the temperature of below 5 DEG C-80 DEG C and then taken out, air thawing is performed at room temperature until the center temperature of the brown algae reaches 0 DEG C-2 DEG C, and then the freeze thawing process is completed; the brown algae are placed into a centrifugal machine and centrifuged for 3-10 minutes at the rotation speed of 800 rpm-10000 rpm, and then water is removed; step2, the step1 is repeated 1-4 times, and a solvent extraction and precipitation method is adopted for preparing high-purity fucoxanthin from the brown algae after freeze thawing treatment is performed. The pretreatment method has the advantages that the fucoxanthin and other effective components in the brown algae can be effectively prevented from being damaged and decomposed, the difference between the water content of the surfaces of the brown alga samples and the water content inside the brown alga samples is small, inside and outside distribution is even, and the water content of the treated brown algae is 50-90%.

The invention provides a carbohydrate composition with a wound healing promoting effect and application of the carbohydrate composition. The carbohydrate composition contains at least five types of carbohydrate including carboxymethyl chitosan, rhizoma bletillae polysaccharides, fucoidan from fucus vesiculosus, heparan sulfate, chondroitin sulfate, oligosaccharides of hyaluronan and gulose aldehyde acid oligosaccharides. The carbohydrate composition and the application have the advantages that VEGFA (vascular endothelial growth factor A), FGF2 (fibroblast growth factor 2) and VE-cadherin protein mRNA (messenger ribonucleic acid) expression can be obviously improved by the carbohydrate composition, VEGF (vascular endothelial growth factor) secretion and skin fibroblast (HSF) and human immortalized keratinocyte (HaCaT) proliferation can be obviously promoted by the carbohydrate composition, and the obvious wound healing promoting effect can be realized by the carbohydrate composition; raw materials required by the carbohydrate composition are easily available, composition methods for the carbohydrate composition are simple, the carbohydrate composition is low in cost, easy to industrialize and wide in application range, and the like.

The invention provides a method for preparing seaweed nutrition powder rich in fucoidin. The method comprises the steps that clean seaweed is chosen and washed with water repeatedly, washing water and the seaweed are collected, the washing water is colated and adsorbed, then the washing water and the clean seaweed are subjected to defibrination, degradation and solvent extraction, and seaweed components from which heavy metals are removed are homogenated, neutralized, concentrated and dried to obtain the seaweed nutrition powder rich in the fucoidin. According to the method, functional components such as the fucoidin, iodine and mannitol in kelp, which can be dissolved in water and can runoff easily during washing, are reserved fully; and different suitable heavy metal-removing methods are adopted respectively based on characteristics of different components, so that the heavy metal-removing effects are guaranteed, and nutrient loss caused by unsuitable removing methods is also avoided.

The present invention discloses an extraction method for fucoidin with a high yield and a high ratio of fucose, belongs to the technology field of algae processing and utilization. According to the present invention, nemacystis is used as a raw material, a high-pressure homogenisation method is used to extract the fucoidin, and therefore extraction time is short, extraction efficiency is high, and retention of natural components content in the fucoidin is maximized. By combining a chemical degradation method and a enzymatic degradation method to degrade the fucoidin, and by ultrafiltration technology, finally low-molecular-weight fucoidin with outstanding anti-tumor activity is obtained. According to the present invention, the yield of the obtained fucoidin is 21%-34%, the purity is 85%-90%, the fucose content reaches 70%-75%, and the organic sulfate ion content reaches 20%-30%.

The invention provides a high-yield fucoidanase degrading enzyme strain. The preservation number of the strain is CGMCC No.13477. The microbiological strain is used for producing fucoidanase, the fucoidanase produced by the screened strain can degrade fucoidan, and oligomerization fucoidanase with potential biological activity is generated. The microorganism has the advantages that the fermentation period is short, the enzyme activity is high, and most activity is intracellular activity, collection and preservation of samples are promoted, and a foundation is laid for subsequent industrial application.

The invention relates to serial methylated fucus evanescens oligose or methylated sulfated fucus evanescens oligose or a salt thereof as well as a preparation method thereof and application thereof in preparing an anti-parkison medicament. The preparation method comprises the following steps of: preparing oligose by carrying out desulphidation esterification onto fucoidan polysaccharide sulfate; purifying and separating by column chromatography to obtain serial high-purity methylated fucus evanescens oligose and methylated sulfated fucus evanescens oligose. The methylated fucus evanescens oligose and methylated sulfated fucus evanescens oligose have a nerve protection effect for nerve cell injuries induced by neurotoxin, and can be used for medicaments and health-care products for preventing and treating neurodegenerative diseases.

The invention relates to the field of a preparation process of fucoxanthin, in particular to a method for improving purity of fucoxanthin in a crude extract of sargassum. According to the method, sargassum is subjected to water extraction at the room temperature, and polysaccharide and mannitol are removed; sargassum with polysaccharide and mannitol removed is mixed with an enzymatic solution for enzymolysis; an organic solvent is added to the sargassum subjected to enzymolysis, and fucoxanthin is obtained after extraction. The fucoxanthin, obtained with the method, in the crude extract of sargassum is high in yield and purity. The method is simple to operate, environment-friendly, efficient and suitable for large-scale operation of extraction of high-purity fucoxanthin from sargassum.

The invention relates to the field of gene engineering, in particular relates to TFLFM (Fucoidan Lyase from Fucobacter marina) as well as a preparation method and application thereof. By shortening anamino acid sequence of TFLFM, the high-efficient expression can be realized. A strain used in the invention is escherichia coli BL21(DE3), and a gene coding the shortened TFLFM is introduced, so thatthe recombinant Escherichia coli of the high-efficiently expressed TFLFM can be obtained, and the enzymatic activity is verified by virtue of in-vitro experiment.

The invention provides an edible composition having the efficacy of enhancing immunity and an application of the edible composition. The edible composition comprises pea isolated protein, hippophae rhamnoides fruit polysaccharide and fucoidin, wherein the pea isolated protein is from pea isolated protein powder as raw materials, the hippophae rhamnoides fruit polysaccharide is from a hippophae rhamnoides fruit polysaccharide extract, and the fucoidin is from a fucoidin extract as the raw materials; and based on effective pea isolated protein, the hippophae rhamnoides fruit polysaccharide and the fucoidin in the raw materials, the mass ratio of the pea isolated protein to the hippophae rhamnoides fruit polysaccharide to the fucoidin is 1 to (0.35-0.6) to (1.1-1.8). The edible composition disclosed by the invention can be added to foods in the percentage of the edible composition to the foods being 1-50%. The edible composition and the foods also have favorable notable effect of enhancing immunity.

The invention discloses a method for improving the content of fucoxanthin in phaeodactylum tricornutum by using purple light. The method comprises the following steps: (1) carrying out inoculating culture: namely inoculating phaeodactylum tricornutum into a culture solution, and carrying out amplification culture in an illumination incubator; (2) carrying out growth measurement: namely measuring the absorbance of the phaeodactylum tricornutum solution every day; and (3) carrying out purple light irradiation: namely carrying out cultivation on the phaeodactylum tricornutum by purple light irradiation when phaeodactylum tricornutum cells grow to a logarithmic phase. According to the method disclosed by the invention, cultivation is carried out on the phaeodactylum tricornutum in the logarithmic phase by purple light irradiation, so that the content of fucoxanthin in the phaeodactylum tricornutum is greatly improved. In addition, the method has simple steps and is easy to operate, the used instrument is relatively normal, and the method is convenient and environmentally friendly.

The invention discloses preparation and activity detection methods of fucoidan components in hizikia fusiformis. According to the key points of the technical scheme, the preparation method comprises the steps that the hizikia fusiformis is washed, dried and crushed, then ethanol is added, reflux degreasing and stirring filtration are conducted, degreased algae powder is extracted repeatedly for three times through a calcium chloride solution, and filtrate is merged to obtain a fucoidan extracting solution; the extracting solution is subjected to depressurizing distillation and concentrated toone fifth of the original volume; anhydrous ethanol is added into a concentrated solution, still standing for the night is conducted, and after centrifugal treatment and cleaning through the anhydrousethanol, vacuum drying is conducted to obtain hizikia fusiformis fucoidan; the hizikia fusiformis fucoidan is dissolved in distilled water, separated through DEAE-cellulose ion exchange column chromatography, eluted with the distilled water and then eluted with 0.1 M of NaCl, 0.3 M of NaCl, 0.5 M of NaCl and 1 M of NaCl, an obtained product is purified through Sepharose CL-6B column chromatography and eluted with NaCl, eluant is analyzed through a phenol-sulfuric acid method, and suitable fractions are collected for preparation. The preparation method has the advantage that preparation of hizikia fusiformis fucoidan components is easy and convenient to operate.

The invention relates to a coextraction method for phaeodactylum tricornutum fucoxanthine and a polyunsaturated fatty acid. The coextraction method comprises the following steps: (1) taking wet alga mud, adding ethanol, performing stirring extraction so as to obtain a mixed liquid of the wet alga mud and the ethanol, and performing solid-liquid separation so as to obtain a pre-extraction liquid and alga cakes 1; (2) mixing the algae cakes 1 with ethanol and normal hexane, performing stirring extraction so as to obtain a double-liquid phase mixed liquid, and performing solid-liquid separation so as to obtain a double-liquid phase extraction liquid and filter cakes 2; and (3) mixing the pre-extraction liquid with the double-liquid phase extraction liquid so as to obtain a mixed liquid, performing layering treatment so as to obtain an ethanol phase and a normal hexane phase, drying the ethanol phase so as to obtain a fucoxanthine extract, and drying the normal hexane phase, so as to obtain a polyunsaturated fatty acid extract. By adopting the method provided by the invention, the fucoxanthine and the polyunsaturated fatty acid can be effectively coextracted, and phaeodactylum tricornutum can be in best use.

The present invention provides for novel fucosidase mutants that server as fuco-ligases for core fucosylation of a range of biological glycopeptides and glycoproteins including intact therapeutic antibodies. Several mutants with mutation at the general acid / base residue E274 of the Lactobacillus casei α1,6-fucosidase, including E274A, E274S, and E274G, were able to efficiently fucosylate a wide variety of complex N-glycopeptides and intact glycoproteins. The site specific mutants enable the transfer of fucose to a core GlcNAc-Asn residue and useful for drug delivery and vaccine development.

The invention provides a method for producing fucoxanthin by using a mutant strain for expressing exogenous pyruvate phosphate dikinase in phaeodactylum tricornutum, and particularly relates to algae preference codon optimization on an original CyPPDK gene to obtain a new CyPPDK gene with a nucleotide sequence as shown in SEQ ID NO.7; the constructed shuttle expression vector is named as pPha-fcp-PPDK; and the obtained new gene is introduced into a host algae cell phaeodactylum tricornutum cell in an electric transformation manner, and a transformant is screened to obtain a phaeodactylum tricornutum transgenic algae strain for overexpression of pyruvate phosphate dikinase. Compared with an unoptimized transformant, the optimized transformant for phosphorylation sites of the CyPPDK gene has the advantages that the biomass accumulation is improved by 33.3%, the content of EPA in grease is improved by 11.5%, and the fucoxanthin yield is improved by 21.2%.

The invention provides a culture method for increasing the yield of fucoxanthin contained in diatom. The culture method comprises the following steps of: (1) heterotrophically culturing activated diatom for 3-8 days so that the diatom is in a logarithmic growth period; (2) inoculating a diatom culture solution obtained from the step (1) in the logarithmic growth period as a seed solution into a heterotrophic culture vessel which contains a heterotrophic culture medium (culture medium I) according to 5%-50% volume of inoculation amount to carry out heterotrophic culture for 4-12 days at the temperature of 20-34 DEG C, wherein tomato extractives are added to the heterotrophic culture medium (culture medium I) obtained from the step (2). The culture method provided by the invention can enhance the biomass concentration of the diatom and increase the fucoxanthin content of a stable diatom body, thereby increasing the yield of the fucoxanthin contained in the diatom.

The invention discloses novel fucoidan oligosaccharides as well as a preparation method and application thereof. The novel fucoidan oligosaccharides comprises a chain formed by connecting four fucose residues in sequence, wherein the fucose residues are alternately chemically combined from 1 to 3 and 1 to 4; the sulfate radical is distributed on C2 and C3 of the second fucose residue and C2 of the third fucose residue. The quantity of the fucose residues (fixed structures) in the fucoidan oligosaccharides provided by the invention is equal to 4 or 6, so that the amount of the fucoidan oligosaccharides with the fixed structures is enlarged, and the use range of the fucoidan oligosaccharides is expanded; more component sources are supplied to medicines for preventing or treating atopic dermatitis and eczema and gastrointestinal infection.

The invention discloses a phaeodactylum tricornutum culture medium. A formula of the culture medium comprises tryptone. Preferably, the formula contains a mixed nitrogen source. The culture medium isfavorable for accelerating the growth speed of the phaeodactylum tricornutum, increasing the cell density and promoting the fucoxanthin accumulation so as to improve the culture efficiency. The phaeodactylum tricornutum culture medium is particularly suitable for large-scale culture.