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Method for improving content of fucoxanthin in phaeodactylum tricornutum by using purple light

A technology of Phaeodactylum tricornutum fucoxanthin and Phaeodactylum sp. is applied in the field of increasing the content of Phaeodactylum tricornutum fucoxanthin by violet light, and can solve the problems of unsatisfactory effect and the like, so as to promote the efficiency of photosynthesis, increase the content and promote the the cumulative effect of

Inactive Publication Date: 2019-07-23
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The economic benefits brought by reasonable artificial light supplements have gradually become prominent, and many researchers have also used artificial light supplements as a convenient and environmentally friendly way to increase the biosynthetic yield of marine algae, such as Zang Zhengrong's "Temperature and Light Effects on Phaeodactylum tricornutum growth and the influence of salinoxanthin content. Red light (647-700nm), blue light (470-475nm) and green light (491-574nm) were used for artificial supplementary light, but the effect was not particularly ideal

Method used

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  • Method for improving content of fucoxanthin in phaeodactylum tricornutum by using purple light
  • Method for improving content of fucoxanthin in phaeodactylum tricornutum by using purple light
  • Method for improving content of fucoxanthin in phaeodactylum tricornutum by using purple light

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Embodiment 1

[0041] The method for increasing the fucoxanthin content of Phaeodactylum tricornutum with purple light specifically comprises the following steps:

[0042] (1) Inoculation culture: take Phaeodactylum tricornutum (provided by the Key Laboratory of Applied Marine Biotechnology of Ningbo University), and use 1 × 10 5 The initial concentration of cells / mL was inoculated into the culture medium and placed in a light intensity of 63 μmol / (m 2 s), the light-dark cycle is 12h:12h, and the cultivation temperature is 24°C in the light incubator to expand the culture. During this period, the algae are shaken 3 times a day, and the position of Phaeodactylum tricornutum is constantly changed to prevent uneven light on the algae. ;

[0043] (2) Growth measurement: take Phaeodactylum tricornutum algae liquid every day, measure the absorbance at 680nm with a UV-5200 ultraviolet spectrophotometer (Shanghai Yuan Analysis Instrument Co., Ltd.) after multiple ratio dilution, and convert it into...

Embodiment 2

[0046] The method for increasing the fucoxanthin content of Phaeodactylum tricornutum with purple light specifically comprises the following steps:

[0047] (1) Inoculation culture: take Phaeodactylum tricornutum (provided by the Key Laboratory of Applied Marine Biotechnology of Ningbo University), and use 1 × 10 5 The initial concentration of cells / mL was inoculated into the culture medium and placed in a light intensity of 63 μmol / (m 2 s), the light-dark cycle is 12h:12h, and the cultivation temperature is 25°C in the light incubator to expand the cultivation. During this period, the algae are regularly shaken 4 times a day, and the position of the Phaeodactylum tricornutum is constantly changed to prevent the algae from being unevenly illuminated. ;

[0048] (2) Growth measurement: take Phaeodactylum tricornutum algae liquid every day, measure the absorbance at 680nm with a UV-5200 ultraviolet spectrophotometer (Shanghai Yuan Analysis Instrument Co., Ltd.) after multiple r...

Embodiment 3

[0051] The method for increasing the fucoxanthin content of Phaeodactylum tricornutum with purple light specifically comprises the following steps:

[0052] (1) Inoculation culture: take Phaeodactylum tricornutum (provided by the Key Laboratory of Applied Marine Biotechnology of Ningbo University), and use 1 × 10 5 The initial concentration of cells / mL was inoculated into the culture medium and placed in a light intensity of 63 μmol / (m 2 s), the light-dark cycle is 12h:12h, and the cultivation temperature is 26°C in the light incubator to expand the culture. During this period, the algae are regularly shaken 4 times a day, and the position of the Phaeodactylum tricornutum is constantly changed to prevent the algae from being unevenly illuminated. ;

[0053] (2) Growth measurement: take Phaeodactylum tricornutum algae liquid every day, measure the absorbance at 680nm with a UV-5200 ultraviolet spectrophotometer (Shanghai Yuan Analysis Instrument Co., Ltd.) after multiple ratio...

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Abstract

The invention discloses a method for improving the content of fucoxanthin in phaeodactylum tricornutum by using purple light. The method comprises the following steps: (1) carrying out inoculating culture: namely inoculating phaeodactylum tricornutum into a culture solution, and carrying out amplification culture in an illumination incubator; (2) carrying out growth measurement: namely measuring the absorbance of the phaeodactylum tricornutum solution every day; and (3) carrying out purple light irradiation: namely carrying out cultivation on the phaeodactylum tricornutum by purple light irradiation when phaeodactylum tricornutum cells grow to a logarithmic phase. According to the method disclosed by the invention, cultivation is carried out on the phaeodactylum tricornutum in the logarithmic phase by purple light irradiation, so that the content of fucoxanthin in the phaeodactylum tricornutum is greatly improved. In addition, the method has simple steps and is easy to operate, the used instrument is relatively normal, and the method is convenient and environmentally friendly.

Description

technical field [0001] The invention relates to the field of algae biotechnology, and more specifically relates to a method for increasing the content of fucoxanthin in Phaeodactylum tricornutum with purple light. Background technique [0002] Fucoxanthin, also known as fucoxanthin or fucoxanthin, is an oxygen-containing derivative of carotene and belongs to the xanthophyll class of carotenoids. Naturally occurring fucoxanthin is mainly distributed in marine invertebrates and photosynthetic algae, especially in brown algae and diatoms. Besides carotene, fucoxanthin is the most abundant algae carotenoid. Fucoxanthin has shown potential value in pharmacological activity. It has been reported that fucoxanthin has anti-inflammatory, anti-tumor, anti-obesity, hypolipidemic, and anti-oxidant effects. and other fields have broad application prospects. With the continuous improvement of human living standards and gradual changes in dietary structure, major diseases such as obesit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N1/12C12P17/02C12R1/89
CPCC12N1/12C12N13/00C12P17/02
Inventor 徐润洁龚一富陈文婷李申睿陈若莹郑小恽陈璇木子王何瑜石慧
Owner NINGBO UNIV
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