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Method for improving purity of fucoxanthin in crude extract of sargassum

A technology of fucoxanthin and sargassum, applied in the direction of organic chemistry, can solve the problems of low fucoxanthin purity and low extraction efficiency, and achieve the effects of saving production cost and production time, increasing extraction rate, and reducing pressure

Active Publication Date: 2017-11-24
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current technology used to prepare fucoxanthin mainly uses a single organic solvent extraction method. This method not only has low extraction efficiency, but also extracts a lot of other impurities such as chlorophyll and mannitol while extracting fucoxanthin. The fucoxanthin in the crude extract has a very low purity (<10%), and must be separated and purified before it can be used in fucoxanthin products (Matt Fucus Light Body Tablets, Italy, fucoxanthin purity 30%; 10% Fucoxanthin Fucoxanthin Fucoxanthin, Shaanxi, China, Fucoxanthin Purity 10%) Production

Method used

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  • Method for improving purity of fucoxanthin in crude extract of sargassum
  • Method for improving purity of fucoxanthin in crude extract of sargassum
  • Method for improving purity of fucoxanthin in crude extract of sargassum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Effect of Particle Size and Water Pre-treatment on Sargassum Fucoxanthin Extraction Rate and Purity

[0033] Weigh 1 g of each of the 4 kinds of sargassum samples (over 100 mesh, over 60 mesh, about 1 mm in length, about 10 mm in length) and add 20 times the volume of deionized water (w (g) / v (ml)), soaked at room temperature for 24 hours, and suction filtered to remove polysaccharides and mannitol in Sargassum algae.

[0034] Add 20 times the volume of deionized water (w / v) to the above-mentioned sargassum from which polysaccharides and mannitol have been removed and 1 g of sargassum that has not undergone water extraction to remove polysaccharides and mannitol (over 100 mesh). , then add 100U / g cellulase and mix well, enzymatically hydrolyze at 37°C for 6 hours, and after suction filtration, place the enzymatically hydrolyzed sargassum in a dark and ventilated place to dry in the shade.

[0035] Add 30 times the volume of methanol (w / v) to the shade-dried ...

Embodiment 2

[0043] Example 2 Comparison of Fucoxanthin Extraction Rate and Purity in Different Sargassum Plant Crude Extracts

[0044] Weigh 1g each of the dried plants of 4 kinds of Sargassum family (Sargassum sargassum, sea millet, sargassum, dark-colored sargassum, and light-colored sargassum), and add deionized water 50 times the volume of the seaweed. (w / v), after soaking at room temperature for 24 hours, suction filtration to remove polysaccharides and mannitol in seaweed.

[0045] Add 20 times the volume of deionized water (w / v) to the above-mentioned groups of algae that have removed polysaccharides and mannitol, and then add 150 U / g cellulase to mix evenly, enzymatically hydrolyze at 37°C for 8 hours, and filter with suction , Place the enzymatically hydrolyzed seaweed in a dark and ventilated place to dry in the shade.

[0046] Add 50 times the volume of methanol (w / v) to the dried seaweed, extract in the dark at room temperature for 3 hours, then filter with suction, and the f...

Embodiment 3

[0052] Example 3 Separation and Purification of Fucoxanthin in the Crude Extract of Sargassum Sargassum Dark

[0053] Weigh 10g of dark-colored Sargassum sargassum dry product, add deionized water (w / v) 50 times the volume of the seaweed, soak at room temperature for 48 hours, and filter with suction to remove polysaccharides and mannitol in Sargassum sargassum .

[0054] Add 20 times the volume of deionized water (w / v) to the above-mentioned Sargassum sargassum from which polysaccharides and mannitol have been removed, and then add 100 U / g cellulase, mix well, enzymolyze at 37°C for 8 hours, and filter with suction Finally, place the enzymatically hydrolyzed Sargassum sargassum in a dark and ventilated place to dry in the shade.

[0055] Add 50 times the volume of methanol (w / v) to the shade-dried Sargassum sargassum, extract in the dark at room temperature for 6 hours, then filter with suction, and the filtrate is concentrated under reduced pressure in the dark and dried in...

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Abstract

The invention relates to the field of a preparation process of fucoxanthin, in particular to a method for improving purity of fucoxanthin in a crude extract of sargassum. According to the method, sargassum is subjected to water extraction at the room temperature, and polysaccharide and mannitol are removed; sargassum with polysaccharide and mannitol removed is mixed with an enzymatic solution for enzymolysis; an organic solvent is added to the sargassum subjected to enzymolysis, and fucoxanthin is obtained after extraction. The fucoxanthin, obtained with the method, in the crude extract of sargassum is high in yield and purity. The method is simple to operate, environment-friendly, efficient and suitable for large-scale operation of extraction of high-purity fucoxanthin from sargassum.

Description

technical field [0001] The invention relates to the field of fucoxanthin preparation technology, in particular to a method for improving the purity of fucoxanthin in crude extracts of Sargassum algae. Background technique [0002] Sargassum is a kind of brown algae, most of which are warm-water species, and are widely distributed in warm water and warm waters. my country is one of the main producing areas of Sargassum. In recent years, due to climate warming, sea warming and other environmental changes, a large number of Sargassum invaded the shoal area and accumulated on the aquaculture rafts, posing a serious hazard to the shoal raft culture. The amount of sargassum floating to the South Yellow Sea is huge. They pose a great threat to the aquaculture industry, the environment and ecology, and may become a new disaster phenomenon. Sargassum floating to the shore will also cause secondary disasters, such as affecting tourism. Therefore, it is particularly important to stre...

Claims

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Application Information

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IPC IPC(8): C07D303/32C07D301/32
CPCC07D301/32C07D303/32
Inventor 邢荣娥杨皓月张恩惠徐超杰刘松李鹏程
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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