Pretreatment method of brown alga samples for fucoxanthin extraction
A technology of fucoxanthin and brown algae, which is applied in the field of pretreatment of brown algae samples, can solve the problems of long operation time, difficulty in realizing the industrial production of fucoxanthin products, and high operating costs, so as to avoid damage and decomposition, good yield and Quality, evenly distributed effect inside and outside
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Embodiment 1
[0025] Freeze the fresh copper algae at a temperature of -20°C, take it out after freezing for 3 days, and thaw it in the air at room temperature, and complete the first freeze-thaw process after the central temperature of the copper algae reaches 0°C; machine, 1000rpm; centrifuge for 10 minutes to remove water; then freeze the sample at a temperature of -20°C, take it out after freezing for 3 days, put it into a centrifuge, 1000rpm; centrifuge for 10 minutes to remove water. Repeat the third freeze-thaw process and centrifugation process. The samples before freezing and thawing, the samples after the first freeze-thaw and centrifugation, the samples after the second freeze-thaw and centrifugation, the samples of copper algae after the third freeze-thaw and centrifugation were taken respectively, and the water content was determined. Determination of fucoxanthin content, calculation of fucoxanthin yield after freezing and thawing. The measurement results are shown in the tabl...
Embodiment 2
[0029] Freeze the fresh sargassum at -10°C, take it out after freezing for 30 days, and thaw it in the air at room temperature, and complete the first freeze-thaw process when the central temperature of the copper algae reaches 2°C; Put it into a centrifuge at 2000rpm; centrifuge for 8 minutes to remove water; then freeze the sample at a temperature of -10°C, take it out after freezing for 30 days, put it in a centrifuge at 2000rpm; centrifuge for 8 minutes to remove water. Complete the fourth freeze-thaw process and centrifugation process repeatedly. Samples were taken before freeze-thaw, after the first freeze-thaw and centrifugation, after the second freeze-thaw and centrifugation, after the third freeze-thaw and centrifugation, and after the fourth freeze-thaw and centrifugation. The sargassum samples were used to measure the water content of the sargassum, the fucoxanthin content in the sargassum, and calculate the yield of fucoxanthin after freezing and thawing. The mea...
Embodiment 3
[0032] Same as the above-mentioned Example 1, the difference is that the fresh sea-tangle is repeatedly frozen and thawed 5 times at a temperature of -80° C., the centrifugation speed is 3000 rpm, and the centrifugation time is 7 minutes.
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