54 results about "Cyanothymidine" patented technology

The invention relates to preparation of a compound 3,4,5,6-tetrachloro-2-cyanopyridine, in particular to a method for catching and purifying 3,4,5,6-tetrachloro-2-cyanopyridine in a gas reaction product in a gas phase chlorination process for producing 3,4,5,6-tetrachloro-2-cyanopyridine. The method comprises the following steps: (1) primary sublimation catching: a reaction gas mixture containing 3,4,5,6-tetrachloro-2-cyanopyridine from a reaction bed enters a first catcher, an inert gas is introduced, the gases are thoroughly mixed and quenched to 135-165 DEG C, and sublimated flocculent crystals fall to a material outlet at the bottom of the first catcher by gravity and are discharged; and (2) secondary sublimation catching: the gas which is not sublimated in the primary sublimation catching enters a second catcher, an inert gas is introduced, gases are thoroughly mixed and quenched to 55-110 DEG C, sublimated flocculent crystals fall to a material outlet at the bottom of the second catcher by gravity and are discharged, and the gas which is not sublimated enters a bubbling absorber. In the invention, the solid product can be further hydrolyzed to obtain high-purity 3,4,5,6-tetrachloropyridine-2-carboxylic acid; and the invention has the characteristic of simple technique, and is easy to operate and control.

This invention relates to novel diaryl ureas of Formula (I), pharmaceutical compositions containing such compounds and the use of those compounds or compositions for treating hyperproliferative and angiogenesis disorders, as a sole agent or in combination with cytotoxic therapies.

The invention discloses a continuous evaporative crystallization device and method for 4-cyanopyridine. The device comprises a pressurizing assembly, a connecting pipe and an evaporation reaction kettle, and is characterized in that the pressure in the evaporation reaction kettle is increased by the pressurizing assembly so as to the crystallization temperature of the 4-cyanopyridine and the boiling point temperature of other organic solvents, after pressurization is completed, a heating resistor is used for heating and crystallizing part of the solution to ensure that only water and other organic solvents are vaporized and the 4-cyanopyridine is heated and crystallized in the evaporation and crystallization process of the 4-cyanopyridine, and meanwhile, water vapor, the other organic solvents and part of the 4-cyanopyridine are absorbed and liquefied again by a condensation reflux box, and drop on the right side of the evaporation reaction kettle, and the heating resistor on the right side carries out evaporative crystallization operation under normal pressure again, so that the 4-cyanopyridine is separated out after being crystallized and is attached to the inner wall of the right side of the evaporation reaction kettle, the crystallization operation steps of the 4-cyanopyridine are simplified, and the crystallization amount of the 4-cyanopyridine separated out is effectively increased.

The invention provides a solid-state acid-base stimulus-responsive near-infrared fluorescent compound 1, the molecular chemical formula of which is shown in formula (I): the invention discloses a solid-state acid-base stimulus-response The preparation method and application of the near-infrared fluorescent compound. Among them, triphenylamine and cyanopyridine are common fluorescent compound units, but the unique chemical structure makes this compound have the advantages of aggregation-induced luminescence characteristics and high solid-state luminescence, and can respond to acid-base stimuli. Under the light, it can be observed that the fluorescence changes from the deep red-near-infrared region at 652nm to the fluorescence emission at 789nm near-infrared I region, with a shift of up to 137nm. In daylight, it can be seen that it changes from red to blue-black. Therefore, the present invention provides a solid-state acid-base stimulus-responsive near-infrared fluorescent material, which has broad application prospects in the fields of stimulus-responsive switch molecular devices, sensing, and anti-counterfeiting.

The invention relates to preparation of a compound 3,4,5,6-tetrachloro-2-cyanopyridine, in particular to a method for catching and purifying 3,4,5,6-tetrachloro-2-cyanopyridine in a gas reaction product in a gas phase chlorination process for producing 3,4,5,6-tetrachloro-2-cyanopyridine. The method comprises the following steps: (1) primary sublimation catching: a reaction gas mixture containing3,4,5,6-tetrachloro-2-cyanopyridine from a reaction bed enters a first catcher, an inert gas is introduced, the gases are thoroughly mixed and quenched to 135-165 DEG C, and sublimated flocculent crystals fall to a material outlet at the bottom of the first catcher by gravity and are discharged; and (2) secondary sublimation catching: the gas which is not sublimated in the primary sublimation catching enters a second catcher, an inert gas is introduced, gases are thoroughly mixed and quenched to 55-110 DEG C, sublimated flocculent crystals fall to a material outlet at the bottom of the secondcatcher by gravity and are discharged, and the gas which is not sublimated enters a bubbling absorber. In the invention, the solid product can be further hydrolyzed to obtain high-purity 3,4,5,6-tetrachloropyridine-2-carboxylic acid; and the invention has the characteristic of simple technique, and is easy to operate and control.

The invention discloses an integrated recombinant mycobacterium smegmatis producing nicotinic acid and a construction method thereof. The invention requires the protection of a recombinant mycobacterium smegmatis producing nicotinic acid, the recombinant mycobacterium smegmatis producing nicotinic acid is characterized in that the recombinant mycobacterium smegmatis contains recombinant integrative plasmid, which contains nudC gene, and the nucleotide sequence is shown as SEQ ID NO:1. Nicotinic acid can be directly obtained by one step through the engineering strain prepared by the invention without adding 3-cyanopyridine, therefore various shortcomings in the existing chemical synthesis process of nicotinic acid can be avoided, and also the method has the characteristics of simple production condition, no pollution, simplicity and feasibility, easy enlargement and low cost, is suitable for large-scale industrial production and application, and has great popularization and applicationvalue.

The invention belongs to pharmaceutical intermediates, in particular to a method for synthesizing methyl 5-amino-3-cyanopyridine carboxylate hydrochloride. The present invention provides a kind of compound A as basic raw material, synthesized 5-amino-3-cyanopyridine carboxylate hydrochloride through three-step reaction, is 5-amino-3-cyanopyridine carboxylate hydrochloride The synthetic method of salt provides synthetic route; The synthetic method of 5-amino-3-cyanopicolinate methyl ester hydrochloride of the present invention is that route is brief, reasonable in design, and simple to operate, easy to control; The obtained product of the present invention obtains The rate is higher.

The invention discloses an Escherichia coli strain and its application in biocatalytic production of low-by-product nicotinamide. The Escherichia coli M910001 described in the invention has a preservation number of CGMCC NO.20430. The Escherichia coli X described in the present invention after the transformation of Escherichia coli M910001 can normally carry out cell metabolism and growth and reproduction in 0-5% 3-cyanopyridine solution, 0-50% nicotinamide solution and their mixed solution, and adapt to the environment Strong ability; after 50 generations of continuous passage, the plasmid retention rate is above 91%, and after 100 generations, the plasmid retention rate is above 80%, which has better plasmid stability.

The invention relates a cyanopyridyl-replaced oxazolidinone compound with a general formula (I); wherein, R1 is hydrogen, fluorine, chlorine or trifluoromethyl and R is shown in the right structural formula. The invention also provides a preparation method of the compound shown in general formula (I) and medicinal salts of the compound and an application thereof for curing microbial infections, in particular to bacterial infection diseases. Preliminary antibacterial activity tests in vitro show that the compound of the invention has better antibacterial activity compared with linezolid and obvious antibacterial activity to drug-resistant bacteria.

The present invention provides a method for constructing a mutant Mycobacterium smegmatis secreting niacin, comprising steps: 1). Constructing plasmid pMHS-NrtR ms ; 2). Construct plasmid pHAGE-NrtR ms ; 3). Preparation of recombinant TM4 phage; 4). Construction of nrtR ms Mycobacterium smegmatis mc 2 100. The beneficial effects of the present invention are: by constructing nrtR ms The gene deletion strain Mycobacterium smegmatis enables the strain to directly prepare nicotinic acid without relying on the addition of 3-cyanopyridine and enzyme catalysis, not only avoiding various shortcomings in the existing chemical synthesis process of nicotinic acid, but also the production conditions Simple, pollution-free, easy to operate, easy to enlarge, low cost, suitable for large-scale industrial production and application, and has great value for popularization and application.