The invention discloses an anti-hair loss essence and a preparation method thereof. The essence includes a thickener, a moisturizing agent, a preservative, a solvent, and an active additive. The active additive includes a compound composed of water, propylene glycol, hydrolyzed pea protein, Complex of 1,3‑Propanediol, Water, Arginine, Lactic Acid, Wild Soybean Germ Extract, Wheat Germ Extract, Scutellaria Baicalensis Root Extract, Sodium Benzoate, Gluconolactone and Calcium Gluconate, Extracted from Turmeric Rhizome , water, gluconolactone, sodium benzoate and calcium gluconate and a complex composed of Ophiopogon japonicus root extract, ginseng root extract, ginger root extract, wild chrysanthemum flower extract, vitex fruit extract, A compound composed of Eclipta eclipta extract, mulberry root bark extract, arborvitae extract, Chuanxiong root extract, Cyperus root extract and Cypress kernel extract. The essence of the invention has the effect of preventing hair loss and growing hair, and can be directly applied to the hair without washing, which simplifies the steps for consumers.