The invention discloses a preparation method of an anti-cat and dog allergen finishing composition solution. The preparation process is as follows: washing the petals of Japanese king persimmon and ylang-ylang ylang-ylang for 3 to 5 times, grinding them, and ultrasonically treating them to obtain a suspension Microwave treatment of the above suspension, followed by centrifugation to obtain a light yellow supernatant; membrane separation treatment of the above light yellow supernatant to form a mixed extract of Japanese Tenno persimmon and ylang-ylang. Mix 2 to 5 parts of Japanese Tenno persimmon and ylang ylang-ylang extract prepared above, 2 to 5 parts of silver ion compound, 5 to 30 parts of eucalyptus oil, 5 to 20 parts of fatty alcohol polyoxyethylene ether, dodecylbenzenesulfonate 5-20 parts of sodium laurate, 0.05-1 part of sodium laurate, 0.05-1 part of sodium stearate, 2-5 parts of citric acid, 0.5-1 part of serine protease, 0.5-1 part of pectinase, 0.5 part of cellulase ~1 part, 0.5~1 part of xylanase, 0.5~1 part of β-glucanase, 20~40 parts of glycerin, 0.05~1 part of hexadecyl dimethyl benzyl ammonium chloride, chitosan 5-10 parts, 0.1-0.5 parts of pigment, 2-3 parts of plant essence, 0.2-1 part of preservative, 20-50 parts of deionized water, mix evenly, ultrasonically oscillate, cool down to room temperature, and then prepare the anti-cat and dog allergen Finish the composition solution. The preparation method of the composition is simple, can be used for daily spraying and wiping, and can also be applied in the process of washing or drying.