165 results about "Coccidioses" patented technology

The invention discloses an E.tenella protein disulfide linkage isomerase gene EtPDI (Clone ID is BW1-E06,and the Genbank accession number of is EF552214). The gene is connected with a procaryon expression vector pGEX-4T-2; a procaryon expression recombination plasmid pGEX-4T-EtPDI is constructed and is expressed in a colibacillus system; and most of the expressed recombining protein exists in a soluble form. The recombining protein 4T-EtPDI is purified to carry out SPS-PAGE and is transferred to a PVDF film; and antiserum of E.tenella oocyst oral immunized chicken is used as first resistance and goat anti-chicken IgG is used as second resistance to carry out Western-blot analysis, thereby indicating that the gene has certain antigen. The gene is used for preparing an anti-chicken coccidiosis drug and an anti-chicken coccidiosis vaccine.

The invention discloses a Chinese medicinal preparation for treating avian coccidiosis and its preparing process, wherein the medicament is prepared from the following constituents (by weight portions): sweet wormwood 200-400g, dichroa febrifuga lour 200-400g, Chinese pulsatilla root 100-300g, astragalus root 100-300g. The preparing process for the product comprises the steps of weighing, poaching, drying, disintegrating, sieving, extracting, filtering, concentrating, flocculating setting, encapsulating and sterilizing.

The invention relates to a kind of slow-releasing parenteral injection and the preparative way of it. The product can prevent coccidiosis of chickens and rabbits. The ingredients of the parenteral injection include: 3-6 shares (by weight) abrotani herba and kuhseng, 2-5 shares (by weight) wood anemone, agrimony and bupleurum root, 2-4 shares (by weight) beta-cyclodextrin. The preparative way of the product goes as follows: lixiviate the materials with 95% ethyl alocohol twice to get A1 solution; extract the residue with water twice to get A2 solution; remove the tannin in A1 and A2 by heating alternating with cooling; envelop the material with beta-cyclodextrin to get the product.

The invention discloses a chicken coccidia powder with low toxicity, high anticoccidial index and low drug application cost and used for resisting chicken coccidiosis, and a preparation method thereof. The chicken coccidia powder contains 400 parts of southernwood, 200 parts of dichroa, 200 parts of verbena, 100 parts of talcum powder, 100 parts of sophorae Flavescentis, and 64 parts of beta-cyclodextrin, by weight; the preparation method of the chicken coccidia powder comprises the steps of: fetching the dried stems and leaves of the southernwood, crushing the dried stems and leaves of the southernwood into crude powder, adding petroleum ether for reflux extraction for four times, fetching and combining filtrate, decompressing-refluxing the petroleum ether to extractum which is then added with 70% ethanol for thermally dissolving and 2.5% activated carbon for reflux decolourization, later conducting concentration to extractum, and drying at temperature of 60 DEG C for obtaining artemisinin; after crushing, mixing with acetone; then dropping the mixture into saturated beta-cyclodextrin at constant speed, stirring, refrigerating, and separating out crystals; conducting suction filtration; washing the sediments with purified water, and drying in a drying cabinet at the temperature of 60 DEG C to obtain an artemisinin-beta-CD clathrate compound; and finally evenly mixing and packing the clathrate compound with the talcum powder and superfine-pulverized dichroa, verbena and sophorae Flavescentis.

The present invention relates to a coccidiosis vaccine to protect poultry against Eimeria infection, comprising a recombinant avian adenovirus vector comprising in frame a (heterologous) promoter linked to a hydrophobic signal sequence for membrane anchoring, or linked to an hydrophobic secretion signal and a cleavage site to allow secretion; a multiple cloning site for in frame insertion of an Eimeria antigen ORF such as derived from the r56, 82 kDa, and / or TFP250 antigens; a polyadenylation signal; and an avian adenovirus genome.

A method for protecting fowl against coccidiosis includes the steps of inoculating the fowl with coccidial oocysts from a strain of Eimeria that is at least partially resistant to an ionophore and subsequently administering to the fowl the ionophore and at least one chemotherapeutic anticoccidial agent. In particular, the ionophore is administered in stages both before and after the administration of the at least on chemotherapeutic agent. In a preferred embodiments, the method provides protection from coccidiosis without administration of antibiotics to the fowl and without continued administration of agents throughout the life of the fowl.

The invention discloses preparing and application of a probiotics-fermented traditional Chinese medicinal preparation for preventing and treating coccidiosis in a chicken. Traditional Chinese medicines comprise artemisia apiacea, antifebrile dichroa, herba verbenae, phellodendron, radix sophorae flavescentis, radix curcumae, cape jasmine, angelica sinensis, pericarpium citri reticulatae, honeysuckle and agrimonia pilosa. Effective traditional Chinese medicinal ingredients are extracted by three times of decoction; afterwards, a traditional Chinese medicinal culture medium substrate is prepared from the effective traditional Chinese medicinal ingredients; lactic acid bacteria PP (pediococcus pentosaceus) are inoculated in the traditional Chinese medicinal culture medium substrate; the inoculated traditional Chinese medicinal culture medium substrate is cultured to prepare the probiotics-fermented traditional Chinese medicinal preparation for preventing and treating the coccidiosis in the chicken. In comparison with the prior art, according to the preparing and the application of the probiotics-fermented traditional Chinese medicinal preparation for preventing and treating the coccidiosis in the chicken, a probiotics-fermented traditional Chinese medicinal aqueous extract is used as a target composition; not only are the further conversion and decomposition of a polysaccharide, starch, cellulose and pharmaceutical active ingredients in the traditional Chinese medicines promoted and are more effective active ingredients are generated, but also the activity of probiotics is improved; the growth and the release of the probiotics and metabolites thereof are facilitated; further, an anticoccidial treatment effect is obviously higher than that of a pure traditional Chinese medicinal preparation.

A method for obtaining an immunogenic strain useful for producing a vaccine against Coccidiosis comprises the cycle of infecting at least one first group of specific pathogen-free donor birds with oocysts from an Eimeria species. Blood is then collected from these donor birds, and is then used to infect a second group of specific pathogen-free birds. Oocysts are collected from the second group of birds. These oocysts are then multiplicated to complete the cycle. The cycle is then repeated using the multiplicated oocysts. After a total of about three cycles, a final antigen may be harvested and utilized as a source to generate oocysts for a vaccine.

The invention relates to a traditional Chinese medicine composition for preventing and treating poultry coccidiosis and a preparation method of the traditional Chinese medicine composition. The medicine materials of the traditional Chinese medicine composition comprise antifebrile dichroa, radix sophorae flavescentis, flos chrysanthemi indici, garlic, cogongrass rhizome, carbonized sanguisorba root, hairyvein agrimony, golden cypress, dandelion, rhizoma atractylodis, fennel, astragalus mongholicus, coptis chinensis and radix bupleuri. By using the traditional Chinese medicine composition, the purpose of preventing and treating coccidiosis in chicken can be effectively achieved in the aspects of expelling parasite, preventing malaria, hemostasis, cooling blood, clearing heat, diminishing inflammation and nourishing. In addition, two technologies of extraction and superfine grinding are adopted in a manufacturing process so that the utilization rate and biological solubility of the traditional Chinese medicines are significantly improved. Water or mixed materials can be taken during administration so that the clinical application is more convenient. Furthermore, the traditional Chinese medicine composition is prepared from pure traditional Chinese medicines, has no drug residue and has no toxic and side effects on an organism.

Echinacea dietary supplements are useful adjuvants for live anticoccidial vaccines. Feed supplementation with Echinacea preparations enhances the immune response to such vaccines. Echinacea purpurea in amounts of about 0.1% to 0.5% administered for about two weeks to day-old chicks has been found effective for providing significant weight gain compared to vaccination alone.

The invention discloses a feed and a traditional Chinese medicine composition for preventing and treating coccidiosis in duck, and a preparation method of the feed. The feed comprises a basic feed, microelement additives and traditional Chinese medicine additives, wherein the traditional Chinese medicine additives comprise areca-nuts, chinaberry barks, common carpesium fruits, fructus ulmi, fructus chebulae, nutmeg, torreya seeds, cyrtomium fortunei, garlic, aloes, dried orange peels, rhizoma atractylodis, poria cocos, fructus aurantii immaturus, radix aucklandiae, toosendan fruits, agrimonia pilosa, callicarpa bodinieri, acalypha australis, humifuse euphorbia, cacumen biotae, reed rhizomes, radix ophiopogonis, justicia procumbens, ixeris chinensis, small-leaved premna roots and memorialis. The feed contains the traditional Chinese additives, has functions of benefiting spleen, relieving diarrhea, removing toxic materials and killing intestinal worms, is used for preventing and treating the coccidiosis in duck, and has the advantages that the gastric mucosa is protected, the duck immunity is improved, the coccidiosis in duck does not easily relapse after being healed, and the cost and the price are low.

Gut health in poultry is improved by providing an efficacious amount of extracts of tannic acid via feed or via drinking water. The extracts of tannic acid are also effective when the poultry are infected with coccidiosis and / or necrotic enteritis. In alternative embodiments, an efficacious amount of Bacillus coagulans is also included.

The invention discloses a preparation method of a live vector vaccine for controlling chicken coccidiosis, comprising the following steps of obtaining a recombinant expression vector by connecting a mature peptide of an eimeria tenella sporozoite antigen gene 3-1E gene and an expression vector of bacillus subtilis, obtaining a recombinant strain by transferring the recombinant expression vector into a bacillus subtilis host, and culturing the recombinant strain to finally obtain the live vector vaccine for controlling the chicken coccidiosis by. The live vector vaccine can be used for controlling and / or treating the chicken coccidiosis. The invention also discloses an oral biological preparation for controlling the chicken coccidiosis, prepared by utilizing the live vector vaccine. The oral biological preparation comprises the live vector vaccine and a vector. The oral biological preparation per gram contains the live vector vaccine with a living bacteria number of 108-109cfu.

This invention relates to an organic iodine complex, in which iodine serves as coordination center and functional group-containing organics serve as chelating reagent. The functional groups can be amino and / or hydroxyl and / or carboxyl, and the representatives of hydroxyl- and carboxyl-containing organics are 2-hydroxy-4-methylthiobutanoic acid and 2-hydroxypropanoic acid, respectively. This invention also relates to a combination of organic iodine complex and its application in pharmacy. The combination comprises 0.3~40% organic iodine complex as active ingredient and one or more ingredients chosen from the following ones: natural aminoacyl iodine, solvent, surfactant, food additives, food and synthesized drugs. This combination can be used in preparation of drug against AIDS, white spot syndrome virus in penaeid shrimp, Taura syndrome virus; grass carp hemorrhage virus, stomatitis, pharyngitis, colpitis, tumor, digestive tract diseases in poultry, histomoniasis, coccidiosis, human digestive tract diseases and iodine deficiency diseases.

The invention relates to a maduramicin ammonium nicarbazin premix and a preparation method thereof. The premix is prepared by dissolving maduramicin ammonium in a corresponding solvent, and then spraying the solution onto nicarbazin and an auxiliary material that are uniformly mixed in a fluidized bed. The solvent can be one or more of a 40%-95% ethanol solution, benzyl alcohol, ethyl acetate and the like. The auxiliary material can be selected from the following: corn starch, plaster stone, corncob powder, anhydrous glucose, talcum powder, and calcium carbonate, etc. The combined use of maduramicin ammonium and nicarbazin can achieve a synergistic anticoccidial effect, not only can inhibit coccidia growth, but also can kill coccidian. The premix is mainly used for prevention and control of chicken coccidiosis. The preparation method of the premix is simple and feasible, has a very good uniform mixing effect, and can effectively avoid various degrees of chicken poisoning phenomena caused by uneven mixing.

The invention discloses a Chinese herbal prescription for treating coccidiosis in chicken, which is prepared from sweet wormwood herb, antifeverile dichroa root, rhynohophylla, quisqualis indica, hairyvein agrimony, sanguisorba officinalis, rhizoma atractylodis, coptis, golden cypress, fructus gardeniae praeparatus, andrographis paniculata, philippine violet herb, dried orange peel and liquorice.The prescription has an obvious inhabiting effect on CVCC-245 bacterial strains of Escherichia coli; and coccidiosis ova artificial infection control experiments show that the effective rate is 94.17-99.17%, the field application and popularization tests show that the effective rate is 95.1%, and the application of the prescription has an obvious control effect and safety in use. The prescriptionhas the efficacies of coccidiosis killing, hemostasis, dampness resolving, spleen invigorating and diarrhea relieving with astringents.

Antigenic polypeptides, capable of inducing an immune response against multiple parasites, and methods of designing such polypeptides, are provided. Also provided by the invention are polynucleotides encoding such polypeptides, as well as recombinant vectors and transformed host cells containing the said polynucleotides. Oral administration and intramuscular injection of the polypeptides provides vaccination protection against infection from Eimeria parasites that result in the poultry disease coccidiosis.

The invention relates to a rhubarb-containing composition and application thereof. The composition comprises the following raw materials or extracts of the raw materials or chemically-acceptable derivatives of the raw material extracts; the raw materials include processed products thereof; and the composition comprises 100 parts by weight of rhubarb, on the basis, and further comprises but is not limited to one or more of antifeverile dichroa root, Chinese thorowax root, sweet wormwood, rangooncreeper fruit, Chinese pulsatilla root, ash bark, lalang grass rhizome, hairyvein agrimonia herb and bud, beautyberry, moutan bark, elecampane inula root and liquoric root which can be added according to specific situations. The composition can be prepared into medicinally or pharmaceutically-acceptable physical forms such as powder, liquids, paste, particles, capsules and the like. The composition can be used for preventing and treating coccidiosis, relieving pathological injuries caused by coccidian, relieving the illnesses, and lowering economic loss.

[Objects] An object of the present invention is to provide an orally administrable agent for medical use for animals for preventing or treating coccidiosis which is highly safe with no side effects, an agent for a food or a drink for use in the preservation of health, a feed additive, and a feed comprising the same. Another object of the present invention is to provide a method for rearing animals (particularly, livestock and poultry) using the same and a method for controlling coccidiosis using the same.[Means for Resolution] By using a fermentation product of a lactic acid bacterium and / or a whey fermentation product of a propionic acid bacterium as an active ingredient, an agent for preventing or treating coccidiosis, an agent for use in the preservation of health, and a feed additive can be provided.

The invention relates to a chicken Eimeria acervulina immunoregulation type DNA vaccine, which belongs to the technical field of biological veterinary medicines. The molecular biology technology is used for connecting lactate dehydrogenase genes of schizont antigen of the first generation of chicken Eimeria acervulina and chIL-2 genes in series to construct a fusion expressing vaccine pVAX-LDH-IL-2. The vaccine comprises a plurality of T cell immune response elements, thus preventing and curing chicken coccidiosis effectively.

The invention discloses a young rabbit feed capable of preventing coccidiosis. The young rabbit feed capable of preventing coccidiosis is characterized by comprising the following raw materials in parts by weight: 300-350 parts of high-quality corns, 100-160 parts of wheat bran, 50-82 parts of bean pulp, 30-52 parts of fish meal, 20-28 parts of bone meal, 8-12 parts of calcium hydrophosphate, 8-11 parts of salt, 55-68 parts of grass meal, 6-9 parts of allicin and 0.5-1 part of diclazuril. The young rabbit feed disclosed by the invention is capable of greatly preventing coccidiosis while providing scientific nutrition for young rabbits.

The invention discloses an anticoccidiosis pharmaceutical composition and a preparation method thereof. The pharmaceutical composition is a traditional Chinese medicine prepared by using fructus cnidii, antifebrile dichroa, radix sophorae flavescentis, radix scutellariae, purslane, hairyvein agrimony, angelica and radix codonopsitis as raw materials. The medicine is used for treating livestock coccidiosis with evident effect, and simultaneously has the advantages of safe use, low production cost, no toxic side-effect and the like.

The invention discloses a feed for preventing and treating coccidiosis of flocks and herds. The feed is prepared from the raw materials in parts by weight: 2-3 parts of ferula asafoetida, 1-2 parts of Chinese honeylocust spine, 2-3 parts of rice grain sprout, 40-45 parts of corn and 50-55 parts of straw. The feed is prepared by the following steps of: firstly, preparing the ferula asafoetida, theChinese honeylocust spine, the rice grain sprout and the corn into powder of 20-40 meshes according to the parts by weight; preparing the straw into particles of 1-4 cm according to the parts by weight, and then uniformly stirring all the raw materials to obtain the product. In the invention, a certain quantity of the Chinese medicinal herbs including the ferula asafoetida, the Chinese honeylocust spine and the rice sprouts is added into the corn and straw feed, so that the feed has the advantages that the morbidity of the coccidiosis of the flocks and herds is efficiently decreased, and the economic loss of raising the flocks and herds is reduced compared with the pure corns and the straws which are used as the feed.

The invention discloses a traditional Chinese medicinal composition for preventing and treating coccidiosis in chicken. The traditional Chinese medicinal composition is prepared from the following components in parts by weight: 5 parts of brucea amarissima, 5 parts of root of Chinese pulsatilla, 5 parts of artemisia apiacea, 3 parts of sanguisorba officinalis, 3 parts of bighead atractylodes rhizome, 3 parts of hairyvein agrimony, 2 parts of liquorice root, 2 parts of radix aucklandiae and 2 parts of radix euphorbiae lantu. The invention also provides a preparation method and application of the traditional Chinese medicinal composition. The traditional Chinese medicinal composition for preventing and treating coccidiosis in chicken as well as the preparation method and the application of the traditional Chinese medicinal composition have the following beneficial effects: (1) the traditional Chinese medicinal composition is capable of effectively preventing the occurrence of the coccidiosis in chicken; (2) the traditional Chinese medicinal composition, which is applied together with drinking water, is simple to operate and is low in cost; (3) the traditional Chinese medicinal composition, as a Chinese herbal medicine preparation, is capable of avoiding such problems as drug resistance and the like, and is capable of greatly reducing failure rate in prevention and treatment; and (4) the traditional Chinese medicinal composition, as a pure traditional Chinese medicinal compound preparation, is low in residue in the chicken and is good in bio-safety.

The invention discloses an immunological regulation type chicken E.tenella resistant recombinant lactobacillus and a preparation method thereof. A recombinant expression vector pW425et-SO7-ChIL-2 with a ChIL-2 gene and a coccidia SO7 gene is constructed by using a DNA (Deoxyribonucleic Acid) recombination technology according to the antigenicity of the SO7, the immunological regulation function of the IL-2 and the security of lactobacillus as an antigen presentation vector, and a corresponding lactic acid bacillus is prepared by using an electric conversion method. The recombinant lactobacillus can be used for remarkably increasing the daily gain of chicken, reducing the cecum lesion after worm attack and reducing the disordered bladder number and the relative disordered bladder yield of the cecum. Flow cytometry detection results prove that the recombinant lactobacillus can be used for regulating the CD4+ / CD8+T cell ratio and has a certain immunological regulation function as comparison with a control group. The preparation method and the production process are relatively simple; and the immunological regulation type chicken E.tenella resistant recombinant lactobacillus is orally taken by drinking water and is convenient for large-scale production and application and wide in application prospects.

The invention aims at solving the technical problems that the traditional medicine for treating coccidiosis has a low medicinal curative effect and residues and affects the quality of poultry products. In order to solve the problem, the invention provides a traditional Chinese medicine composition for preventing and controlling poultry coccidiosis and a preparation method of the composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 80-160 parts of artemisia apiacea, 80-160 parts of antifebrile dichroa, 60-120 parts of radix bupleuri, 60-120 parts of Chinese pulsatilla root and 60-120 parts of astragalus membranaceus. The traditional Chinese medicine composition can effectively treat and prevent the poultry coccidiosis, is conveniently used, and does not have toxic or side effect and medicine residues after the medicine is admisitered.

The invention discloses a preparation method of an eimeria tenella recombinant polypeptide vaccine VNQS. The preparation method comprises the following steps: preparing raw materials; the method comprises the following steps: 1, cloning a VKVQ encoding gene, designing an upstream primer shown in the formula SEQ ID NO: 3 and a downstream primer shown in the formula SEQ ID NO: 4 according to a chicken coccidiosis gene sequence, carrying out PCR amplification and electrophoresis to obtain a segment of about 1500bp, carrying out ligation transformation on the product to obtain VNQS of which the sequence overall length is 1452bp and of which the sequence is shown in the formula of SEQ ID NO: 1, and carrying out subsequent related steps. The vaccine prepared by the method can effectively controlchicken bodies to be infected with eimeria tenella by immunizing eimeria tenella recombinant polypeptide vaccine VNQS eukaryotic expression plasmids and prokaryotic expression proteins, so that the use amount of anti-coccidiosis drugs in chicken farms is greatly reduced, and chicken coccidiosis is effectively controlled.

The invention discloses a fusion protein for resisting chicken coccidiosis, a preparation and an application thereof. The invention provides a fusion gene and its expressed fusion protein, wherein the nucleotide sequence of the fusion gene is as shown in SEQ ID NO.1. The invention also constructs a recombinant plasmid containing the above fusion gene. The fusion protein provided by the invention can be used to prepare drugs for preventing chicken coccidiosis.