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Methods and compositions for use of a coccidiosis vaccine

A technology for coccidiosis and vaccines, applied in the field of utilizing coccidiosis vaccines and compositions, which can solve the problems that the vaccination mode is not easy to apply directly to large broiler flocks, etc.

Inactive Publication Date: 2012-01-25
VECTOGEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this mode of vaccination is not easily applicable for direct administration to large broiler flocks

Method used

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  • Methods and compositions for use of a coccidiosis vaccine
  • Methods and compositions for use of a coccidiosis vaccine
  • Methods and compositions for use of a coccidiosis vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] Example 1: Preparation and Analysis of Constructs

[0093] In this example, one of the most immunogenic proteins from the megagametocyte stage of the Eimeria parasite, recombinant protein r56, was used. Cloned into the FAV8 vector. In addition, another immunogenic protein from Eimeria egg mass development, the recombinant protein-TFP250, was cloned separately into another FAV8 vector. This TFP250 ORF encodes part of a microneme (an organelle involved in parasitic invasion) protein that was shown in previous studies to also induce partial protective immunity against Eimeria infection challenge .

[0094] The resulting expression constructs are shown in the two tables below.

[0095] Part of the R56 gene used in the coccidiosis construct:

[0096]

[0097] Part of the TFP250 gene used in the coccidiosis construct:

[0098]

[0099]

[0100] To maximize the immune response in chickens using this vector system, each ORF encoding the two proteins was cloned...

Embodiment 2

[0111] Example 2: Induction of a protective immune response

[0112] This example tests the ability of FAV8-r56 and FAV8-TFP250 vectors to induce protective immunity that blocks Eimeria maxima development and protects against parasite lesions.

[0113] Table 2:

[0114] Group

deal with

A

Not vaccinated

B

20,000 Eimeria giga oocysts per chicken

C

40,000 Eimeria giga oocysts per chicken

D

80,000 Eimeria giga oocysts per chicken

[0115] To assess the number of oocysts required to develop lesions, a pilot experiment was performed in which 30 SPF chickens were reared in coccidiosis-free conditions (cleanliness, water and feed) in portable clean cages. Coccidia infection was prevented by weekly treatment with amprolium via drinking water prior to challenge. On day 28 an appropriate number of Eimeria maxima oocysts was administered orally (see Table 2). Lesion scoring was performed on day 34 (at least 5 bir...

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Abstract

The present invention relates to a coccidiosis vaccine to protect poultry against Eimeria infection, comprising a recombinant avian adenovirus vector comprising in frame a (heterologous) promoter linked to a hydrophobic signal sequence for membrane anchoring, or linked to an hydrophobic secretion signal and a cleavage site to allow secretion; a multiple cloning site for in frame insertion of an Eimeria antigen ORF such as derived from the r56, 82 kDa, and / or TFP250 antigens; a polyadenylation signal; and an avian adenovirus genome.

Description

[0001] related application [0002] This application claims priority to US Provisional Patent Application 61 / 122,596, filed December 15,2008. The entire text of the above application is incorporated by reference into this application. field of invention [0003] The present invention relates to methods and compositions for vaccinating poultry. Background of the invention [0004] Coccidiosis is an extremely important chicken disease in the world. The disease is estimated to cost the broiler industry alone more than $1 billion per year. Coccidiosis is caused by infection with seven species of the protozoan parasite Eimeria of the apicomplexan phylum. Of the seven species, E. tenella, E. giga, and E. acuminata were considered the most problematic. Symptoms of coccidiosis include lethargy, anemia, watery or bloody diarrhea (depending on the species infested), weight loss and poor feed conversion. [0005] The growth and spread of Eimeria parasites is particularly prevalent...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/861A61K39/012A61K39/235A61P33/02C12N7/01A61K39/00
CPCA61K2039/53A61K2039/552A61K39/012A61P33/00A61P33/02A61K39/235C12N15/861C12N7/00
Inventor M.G.谢波德
Owner VECTOGEN
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