Triple oral vaccine for cultivating marine fishes as well as preparation method and use method thereof

Abstract

The invention relates to triple oral vaccine for cultivating marine fishes. The triple oral vaccine is prepared by mixing pharmaceutical starch, maltodextrin, vibrio parahaemolyticus broth with the concentration of 1*1012cfu, vibrio alginolyticus broth with the concentration of 1*1012cfu and edwardsiella tarda broth with the concentration of 1*1012cfu in the ratio of 3 to 1 to 0.35 to 0.35 to 0.35 according to the unit mode being g:g:ml:ml:ml. The invention relates to a preparation method of the triple oral vaccine, comprising the following steps of: (1) inactivating; (2) preparing pellets; (3) preparing an enteric coated layer lagging coating; and (4) coating phagostimulant in a spraying way. The invention relates to a use method of the triple oral vaccine, wherein the use method comprises the following steps of: (1) firstly feeding and (2) intentinonally feeding. According to the preparation method provided by the invention, the vibrio parahaemolyticus broth, the vibrio alginolyticus broth and the edwardsiella tarda broth trigeminy oral vaccine can be prepared. A feeding and vaccinating method is used, so that the triple oral vaccine is ideal in immune effect, and small in workload, thereby being suitable for vaccination on a large scale. Meanwhile, the triple oral vaccine provided by the invention adopts a functional coating material, so that the vaccine can not be digested within the gastric juice, and the vaccine can be quickly released within the intestinal tract; and therefore, the immune response can be generated, and the high-efficiency immune effect can be obtained.

Description

technical field [0001] The invention belongs to the technical field of vaccines for aquaculture seawater fish and their use, and in particular relates to a triple oral vaccine for aquaculture seawater fish and its preparation and use methods. Background technique [0002] The diseases of marine cultured fish in my country are increasing year by year, especially the prevalence of Vibrio parahaemolyticus, Vibrio alginolyticus and Edwardsiella tarda, which have caused huge economic losses and seriously hindered the development of aquaculture in my country. At present, fish bacterial diseases are mainly prevented and treated by using antibacterial drugs combined with optimized breeding techniques. However, the long-term use of antibiotics and chemicals has caused most bacteria to develop strong and extensive drug resistance, and has also caused food safety problems such as environmental pollution and excessive drug residues. Due to the problem of drug residues, the export of aq...

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Problems solved by technology

However, the above techniques have not studied the physiological characteristics of the gastrointestinal tract of related fish, nor have they conducted acid resistance research, so the active ingredients of the vaccine cannot achieve the purpose of intestinal release, such as cultured seawater fish, such as turbot, For flounder, etc., the pH of gastric juice is generally 2.5-5.5, the gastric emptying time is generally 4-8 hours, and the pH of intestinal juice is generally 6.0-7.8. Because fish vaccines are easily affected by temperature, pH, and pepsin, direct oral vaccination will damage Vaccines reduce the immune effect of vaccines. If the vaccines are released prematurely, they will be decomposed by gastric acid and digestive enzymes, and cannot play an immune protective role, which limits their application in production

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