279 results about "Cell brain" patented technology

Methods and assay systems for analyzing effects of chemical and cellular agents on brain cell neurogenesis in vivo, comprising administering an agent to a test animal and determining responses of cells of brain marrow tissues, including irradiated brain marrow tissue depleted of neurogenic stems cells, and cells in brain marrow-derived neurospheres cultured in vitro.

This invention provides methods for alleviating seizure disorders in an animal, particularly epilepsy, by regulating the flux through the gluconeogenic enzyme PEPCK in brain cells.

The invention relates to a 7 and 20 dehydro-silybin dialky ether and preparation method and medicine use thereof. The compounds are capable of obviously preventing the lipid superoxide induced by the free radical from being generated; effectively protecting the injury rat adrenal pheochromocytoma cells PC12 due to hydrogen dioxide, namely with oxidation resistance injury protection function on thePC12 cells which simulate the cranial nerves; preventing the brain cells and the cranial nerves from being oxidized and controlling the neurodegenerative diseases such as senile dementia. In addition, the compounds have strong chelation on the ferrous ion. The pharmacodynamics result shows that: the compounds are anticipated to prepare the medicine for controlling the neurodegenerative diseases.

The invention relates to Isopentenyl-oxyl substituted dehydrogenized silybin ether and the preparation method and the application thereof. The compound has the activity of eliminating extracorporeal extracorporeal 1-diphenyl-2-picrylhydrazyl radical, the activity of obviously inhibiting the generation of lipid peroxide induced by radical and the function of powerfully preventing the damage of therat adrenal pheochromocytoma PC12 cells due to the hydrogen peroxide, i.e. the function of preventing the oxidative damage of the PC12 cells simulating the brain nerve cells, thereby playing a positive role in preventing the brain cell tissue oxidation and brain never oxidation and preventing and treating the senile dementia and other neurodegenerative diseases. The pharmacodynamic result shows that 7-site substituted dehydrogenized silybin ether can be expectantly used for preparing the medicament for preventing and treating the neurodegenerative diseases.

Activated protein C (APC), prodrug, and / or a variant thereof may be used as an inhibitor of apoptosis or cell death and / or a cell survival factor, especially for stressed or injured cells or tissues of the nervous system including subjects with neurode-generative disorders. Novel biological functions (e.g., neuroprotection) can be independent or separated from inhibition of clotting or inflammation, and other biological properties of APC (e.g., antithrombotic activity, ability to reduce NFκB-regulated gene expression). It can be used in the treatment of disease or other pathological conditions by at least inhibiting the p53-dependent and / or caspase-3-dependent pro-apoptotic signaling pathways in stressed or injured cells. Thus, APC, prodrugs, and variants thereof (e.g., APC protease domain mutants with reduced anti-coagulant activity) are prototypes of a class of agents for preventing apoptosis or cell death and / or promoting cell survival by direct action on brain cells. New protein C and / or APC variants with reduced anticoagulant activity may be selected thereby.

The invention features methods for increasing or maintaining the number of functional cells of a predetermined type, for example, insulin producing cells of the pancreas, blood cells, spleen cells, brain cells, heart cells, vascular tissue cells, cells of the bile duct, or skin cells, in a mammal (e.g., a human patient) that has injured or damaged cells of the predetermined type.

There is provided a method of promoting neurogenesis by administering a therapeutic amount of a nitric oxide donor compound to a patient in need of neurogenesis promotion. Also provided is a compound for providing neurogenesis having an effective amount of a nitric oxide donor sufficient to promote neurogenesis. A nitric oxide compound for promoting neurogenesis is also provided. Further, a method of augmenting the production of brain cells and facilitating cellular structural and receptor changes by administering an effective amount of a nitric oxide donor compound to a site in need of augmentation is provided. There is provided a method of increasing both neurological and cognitive function by administering an effective amount of a nitric oxide donor compound to a patient.

The invention discloses a construction method of a mandarin fish brain cell system. Aiming at the characteristic of mandarin fish brain cells, the primary cell culture is carried out by separating the brain cells through a serum-contained collagenase digestion method, so that the mandarin fish brain cell system is constructed successfully. Thus, a great quantity of mandarin fish brain source cells can be provided; and the good growth status of sub-culture cells can be maintained. The construction method is strong in repeatability and brings convenience for researching fish virus infection paths and fish virus infection mechanisms, developing viral vaccines and the like.

The invention can be characterized as method for stimulating or inhibiting gene expression by photomodulating living cells using a source of narrowband multichromatic electromagnetic radiation. The cells may include, among others, nerve cells, skin cells, retinal cells, heart cells, stem cells, brain cells, cells found in human organs, cells found in hair follicles, and cells found in the human eye or retina. Photomodulation may be enhanced using topically or orally administered compositions. The source of narrowband multichromatic electromagnetic radiation may include at least one light emitting diode (LED) that can emit radiation having a wavelength of from about 300 nm to about 1600 nm.

This invention provides methods for alleviating paroxysmal disorders in an animal, particularly epilepsy, by modulating glycolysis in brain cells.

A method of reducing the occurrence of brain cell damage or death caused by transient cerebral hypoxia / ischemia condition or a traumatic brain injury (TBI) event. The method typically comprises identifying a subject with a transient cerebral hypoxic and / or ischemic condition, or a TBI, and within 24 hours of onset of the condition, administering to the subject a continuous intravenous infusion dose of methamphetamine in an amount sufficient to reduce the occurrence of brain cell damage or death caused by the condition. Preferably, in addition to the continuous intravenous infusion dose, a bolus dose of methamphetamine is administered to the subject as soon as possible after onset of the condition or occurrence of the TBI event.

The invention provides conjugates, comprising an organ, tissue or tumor cell homing molecule linked to a moiety. Such a moiety can be, for example, an oligonucleotide, small interfering RNA, gene, virus, protein, pharmaceutical or detectable agent. In addition the invention provides methods to diagnose or treat neuronal or neuromuscular disease, or a pathology of the brain, or a tumor of neuronal or neuroectodermal origin, by administrating to a subject having or suspected of having a pathology a molecule or conjugate that homes to, binds to and is taken up by the brain cells or neuronal cells, or by the tumor cells of neuronal or neuroectodermal origin. The invention also provides a method of identifying and measuring neurite growth in neuronal cells.

A brain to brain interface system has a brain activity detection device configured to detect activity state information of a brain, a brain stimulation device configured to stimulate an area of at least a part of the brain to activate or inactivate brain cells of the corresponding area, and a computer configured to control the brain activity detection device and the brain stimulation device, wherein brain activity state information of a subject's brain (“a target brain”) is obtained through the brain activity detection device, and an area of at least a part of the target brain is stimulated through the brain stimulation device based on the brain activity state information of the target brain to regulate a function of the target brain.

There is provided a treatment for patients suffering from neurodegenerative disease or neural injury including the steps of transplanting cultured bone marrow cells into the spinal cord or brain or injecting intravascularly bone marrow cells of a patient in need. Also provided is a method of activating the differentiation of neural cells in an injured brain including the steps of transplanting bone marrow cells adjacent to the injured brain cells and activating the endogenous central nervous system stem cells to differentiate into neurons. A method of treating injured brain or spinal cord cells is also provided including the steps of transplanting bone marrow cells near the injured brain cells and generating new neurons at the location of transplantation. A method of treating injured brain or spinal cord cells with a composite of MSCs and neurospheres.

There is provided a method of promoting neurogenesis by administering a therapeutic amount of a nitric oxide donor compound to a patient in need of neurogenesis promotion. Also provided is a compound for providing neurogenesis having an effective amount of a nitric oxide donor sufficient to promote neurogenesis. A nitric oxide compound for promoting neurogenesis is also provided. Further, a method of augmenting the production of brain cells and facilitating cellular structural and receptor changes by administering an effective amount of a nitric oxide donor compound to a site in need of augmentation is provided. There is provided a method of increasing both neurological and cognitive function by administering an effective amount of a nitric oxide donor compound to a patient.

The present invention is one kind of Chinese medicine composition for treating cerebral hemorrhage and is preparation process. The Chinese medicine composition consists of effective components from motherwort, giant knotweed, leech, gentian, and buffalo horn or rhinoceros horn; and may be prepared into medicine powder, tincture, capsule, tablet, micro pill and other forms. The Chinese medicine composition has the function of promoting blood circulation to disperse blood clots, and can alleviate hemorrhagic apoplexy cerebral edema, promote the absorption of hematoma and disease focus repair, improve the ischemic and anoxic state of nerve tissue around hematoma, protect damaged brain cell and promote restoring of the nerve function.

The invention discloses a targeting ginkgolide B solid lipid nanoparticle and a preparation method thereof. The ginkgolide B solid lipid nanoparticle targeting a blood brain barrier is prepared with an oil-in-water emulsion process by taking a ginkgolide B as a treating medicament, taking a solid lipid material as a carrier, taking a folic acid-modified surfactant as a targeting material and taking a mixture obtained by mixing a surfactant with the folic acid-modified surfactant in a certain ratio as an emulsifier. The particle diameter is 80-200 nanometers, and the polydispersion coefficient is 0.30+ / -0.10. According to the solid lipid nanoparticle, a brain targeting effect is achieved by using the folic acid-mediated phagocytosis on the surfaces of brain cells and the phagocytosis way of adsorption of a brain cell membrane by using the nanoparticle.

The present invention relates to a composition for preventing and treating dementia and memory impairment, which contains minocycline as active ingredient. The composition of the present invention has an effect of inhibiting brain cell death and memory impairment, which are induced by amyloid beta-protein and C-terminal protein. Thus, the composition of the present invention is useful for the prevention and treatment of various dementias, including Alzheimer's disease, and the impairment of learning and memory and cognitive function.

The invention provides conjugates, comprising an organ, tissue or tumor cell homing molecule linked to a moiety. Such a moiety can be, for example, an oligonucleotide, small interfering RNA, gene, virus, protein, pharmaceutical or detectable agent. In addition the invention provides methods to diagnose or treat neuronal or neuromuscular disease, or a pathology of the brain, or a tumor of neuronal or neuroectodermal origin, by administrating to a subject having or suspected of having a pathology a molecule or conjugate that homes to, binds to and is taken up by the brain cells or neuronal cells, or by the tumor cells of neuronal or neuroectodermal origin. The invention also provides a method of identifying and measuring neurite growth in neuronal cells.

The present invention relates to a noodle with the functions of brain tonic, mentality improving and health preserving, and a production process thereof, the raw material of the noodle is prepared by raw materials of 80 to 86 wt% of wheat flour, 10 wt% of black hen eggs, 2 wt% of strain and 10 to 18% of functional materials. The functional materials include qi-tonifying materials of yam, blood-enriching matter of tuber fleeceflower root, liver wind-extinguishing materials of gastrodia tuber, heart-nourishing and nerve-soothing matters of arborvitae seed, galangal fruit seed, tuckahoe and longan, resuscitation-inducing and fragrant matter of acorus calamus, diuresis and dampness-excreting matter of tuckahoe, interior-warming matter of common fennel fruit and astringing matter of oyster, with each functional matter taking account for 1 to 1.5%. The production process of the invention comprises the following steps of raw material inspection, drying, grinding, mixing, biological fluids preparing, bio-fermentation, debitterizing, mixing, noodle pressing, drying and packaging. The present invention combines the ancient anaerobic subterranean pot natural zymotechnics with modern aerobic zymotechnics, creatively invents a novel quantum biological multi-fermentation process which produces plentiful alkaline polysaccharide to activate brain cell.

The invention relates to high purity gangliosides preparation method. It includes the following steps: using higher animal brain cell as raw material; adding solvent to homogenate and do de-lamination; processing gnagliosides extracting solution by once new type macroporous resin column to gain the gangliosides which purity is more than 98%. The known gangliosides compound has extensive biological activity. And its main function is to cure and improve impaired neurological disease. The invention adopts new type macroporous resin for the first time, utilize resin structure to remove harmful substance, separate effective constituent, and keep its biological activity. It is fit for industrialization production.

The present invention relates to stilbene glycosie compound and the use of its derivative and medicinal salt in preparing medicine for dementia, especially for Alzheimer disease and vascular dementia. Animal experiment proves that stilbene glycoside compound can resist brain cell damage caused by beta-amyloid protein and hydrogen peroxide, raise the memory capacity of mouse model with scopolaminedementia and A beta dementia and gerbille model with double-sided common carotid artery obstruction caused brain ischemia, inhibit the lipide peroxidation of brain tissue of mouse model with brain ischemia, strengthen the activity of its brain tissue SOD, and reduce the calcium ion density in its brain cells.

The invention discloses a method for establishing a bastard halibut brain tissue-derived cell system. The method comprises brain tissue primary culture, subculture, cryopreservation, recovery and identification, wherein cell culture fluid adopted is obtained by adding fetal calf serum and human basic fibroblast growth factor to basal cell culture fluid. The established bastard halibut brain cell system is represented as an epithelioid-like astrocytic cell, which can support continuous passage to provide a great amount of bastard halibut brain cells and can be directly used for research of bastard halibut functional gene. The construction method disclosed by the invention not only is expected to serve as a platform for theoretical research of bastard halibut molecule cellular level but also can be used as an ideal material for research on fish hemadenology, reproductive biology and environmental internal-secretion interfering-substance toxicology.

The invention discloses a constructing method and application of a koi brain cell line. The constructing method includes: placing koi brain tissue in digestive liquid A containing collagenase and trypsin for digestion; collecting cells; adding into digestive liquid b containing EDTA-4Na for digestion; performing primary culture and secondary culture after the cells which are digested are collected to obtain the koi brain cell line. Aiming at features of koi brain tissue cells, a digestion method combining collagenase with trypsin is adopted to separate tissue cells for primary culture, the koi brain cell line is constructed successfully and continuously transferred to more than 100 generations already, and a lot of koi brain source cells can be provided; the cells can maintain good growing state, so that the cells can be freeze-stored. The koi brain cell line is sensitive to koi herpes viruses with cytopathy occurring after being exposed to the viruses, and proliferated virus particles can be found through electron microscope observation. And the koi brain cell line can be directly applied to pathogenic characteristic study and vaccine development.

A pillow with better neck support structure in accordance with the present invention comprises: a head support portion connected to a correction support portion, the head support portion is softer than the correction support portion. The user's head can be well positioned when laid on the head support portion which is softer than the correction support portion, and the correction support portion which is relatively harder can support the user's head in such a manner that the scapula of the user is supported on the second support section of the correction support portion, while the user's neck is suspended with respect to the traction section, so that the cervical spine, under its gravity, can be returned to a pressure-free and most ergonomic position, thus improving blood circulation in the brain cells.

Methods and a computer program product for applying deconvolution to spatial light interference microscopy for resolution enhancement with respect to the diffraction limit in two and three dimensions. By exploiting the sparsity properties of the phase images, which is prominent in many biological imaging applications, and modeling of the image formation via complex fields, the very fine structures can be recovered which were blurred by the optics. The resolution improvement leads to higher accuracy in monitoring dynamic activity over time. Experiments with primary brain cells, i.e. neurons and glial cells, reveal new subdiffraction structures and motions. This new information can be used for studying vesicle transport in neurons, which may shed light on dynamic cell functioning. Finally, the method may flexibly incorporate a wide range of image models for different applications and can be utilized for all imaging modalities acquiring complex field images.