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34 results about "Aminoacid analyzer" patented technology
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Biomedical equipment or process used to determine the quantities of each individual amino acid in a protein.
Method for detecting 18 varieties of protein hydrolytic amino acids in milk powder through high-performance liquid chromatographic method
The invention belongs to the technical field of amino acid detection, in particular relates to a method for detecting 18 varieties of protein hydrolytic amino acids in milk powder through a high-performance liquid chromatographic method, solving the problems that the traditional amino acid determination method has high cost and poor universality and the qualitative and quantitative analysis of the 18 varieties of standard amino acids after proteolysis cannot be quickly and accurately performed through a high-performance liquid chromatograph at present. The method comprises the steps of: pre-treating the milk powder through three different hydrolysis methods; performing pre-column derivatization of a hydrolyzed milk powder smaple through a CNBF (4-chloro-3,5-dinitrobenzotrifluoride) derivatizing agent; optimizing derivatization conditions and chromatographic fractionation detection conditions by adopting the HPLC (High-performance Liquid Chromatography) and a universal C18 (250mm*4.6mm, 5mu m) liquid chromatography column; and separating and determining all 18 varieties of proteolyzed amino acids in the milk powder. The method has the advantages of stronger universality and no need of the precious amino acid analyzer and / or the precious amino acid determination reagent kit, thereby being suitable for more common laboratories.
Owner:山西出入境检验检疫局检验检疫技术中心
Compounded amino acid injection 18AA-II content measurement method
ActiveCN105588900AEasy to separateReduce distractionsComponent separationAmino Acid InjectionAmino acid content
The invention relates to the field of medicine preparation and especially relates to a compounded amino acid injection 18AA-II content measurement method. According to the method, when the time of conversion of a buffer solution from B2 to B3 is pushed back by 0.5 min, the time of conversion of a buffer solution from B3 to B4 is pushed back by 0.8 min, and the temperature of a separation column reaches 57 DEG C, a better separation effect between the components is achieved and further separation degree between cystine and valine satisfies the demand on content measurement, and meanwhile, interference on the cystine due to conversion of a mobile phase is reduced, so that accurate quantitative measurement is achieved even under a low content, and finally, accurate measurement of contents of sixteen amino acids in the compounded amino acid injection 18AA-II with an amino acid analyzer can be achieved.
Owner:HUAREN PHARMACEUTICAL CO LTD
Method for quantitatively detecting sulfur-containing amino acid in sludge
The invention discloses a method for quantitatively detecting sulfur-containing amino acid in sludge. The method includes: performing freezing drying, grinding and sieving on the sludge to obtain a sample; using formic acid to convert the sulfur-containing amino acid in the sludge into cysteic acid and methionine sulfone; using concentrated hydrochloric acid to hydrolyze protein, and using a 3kD filter membrane to perform ultrafiltration, immunity removing and purification on hydrolysate; using phthalic dicarboxaldehyde as the derivative agent to allow the sulfur-containing amino acid derivatives to have fluorescent characteristics; using high-performance liquid chromatography to perform quantitative analysis on the derivatives. Compared with a conventional method, the method has the advantages that the method is simple and convenient to operation, low in toxicity, high in detection accuracy, high in sensitivity and good in repeatability, an expansive amino acid analyzer is not needed, and the like.
Owner:TONGJI UNIV
Breeding method and preparation method of new variety of health-care boxthorn leaf tea
InactiveCN103749289AIncrease productionImprove disease resistanceTea substituesPlant genotype modificationDiseaseBetaine
The invention discloses a breeding method and a preparation method of a new variety of health-care boxthorn leaf tea. A squashing technique is adopted for cytological observation; an over-parent heterosis method and an over-contrast dominant method are utilized to determine the growth advantages of the leaf area, the leaf thickness and the stem diameter of a mutant, the length of an edible part and the fresh weight of tender stems and leaves; a region production testing method is adopted to determine the yield performance of the new variety; a disease index method is used for identification of disease resistance; a semimicro kjeldahl method is adopted to determine the content of crude protein; a Soxhlet extraction method is utilized to determine the content of crude fat; an atomic absorption method is used for determining the contents of calcium, potassium, magnesium, sodium, phosphorus, copper, iron and the like; a fluorescence spectrophotometry is adopted to determine the content of selenium; an L-8500A amino acid analyzer is adopted to determine the content of amino acid; an ultrasonic extraction method is utilized to determine the contents of lycium barbarum polysaccharide and flavone; a spectrophotometer is used for determining the content of glycine betaine; the bred multiploid new variety has strong growth vigor, stable hereditary, super high yield, strong root rot resistance, high nutrition and good medicine quality, and the produced boxthorn leaf tea has high quality.
Owner:HEBEI NORMAL UNIVERSITY OF SCIENCE AND TECHNOLOGY
Method for measuring content of amino acid in black-bone chicken milk increasing preparation
InactiveCN105223280AAccurate determination of contentControl internal qualityComponent separationHydrolysateTotal nitrogen
The invention discloses a method for measuring the content of amino acid in a black-bone chicken milk increasing preparation. The method comprises the following steps: subjecting a black-bone chicken milk increasing preparation to a protein hydrolysis treatment; and then using an amino acid analyzer to analyze the hydrolysate so as to measure the amino acid content. In the conventional standards, the protein content can be only represented by total nitrogen content, the provided method can overcome the shortage, moreover, the method can precisely measure contents of 17 amino acids in the preparation, has the advantages of simple pretreatment, strong specificity, good repeatability, and high precision, can well control the quality of the black-bone chicken milk increasing preparation, and improves the safety and effectiveness of the preparation.
Owner:天津飞鹰玉川药业有限公司
Method for establishing konjak fingerprint pattern
The invention relates to a method for building a konjak finger print; the method adopting a high efficiency liquid phase chromatography includes the following steps: (a) the preparation of a sample: konjak tissues are taken for deactivation of enzymes, drying and trituration and then weighed in a test tube, and the constant volume and filter of the konjak tissues are made by purified water, and the sample undergoes the pre column derivation according to the analysis method for plant amide acids; (b) chromatogram conditions: the high efficiency liquid phase chromatograph or the amino acid analyzer is used, and the chromatographic column uses the octadecylsilane chemically bonded silica as fillers; (c) the detection: the sampling solution is precisely sucked and injected in the high efficiency liquid phase chromatograph, and the finger print is detected and obtained according to the high efficiency liquid phase chromatography. By analyzing the relation between characteristic peaks in the finger print and the breed, the quality, the resistance, the output and the growth state, etc. of the konjak, the invention can provide technical directions for scientific efforts such as the collection and classification of the konjak resource, the breeding selection of new breeds and the research of novel amino acid pesticides for controlling konjak diseases, etc.
Owner:ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
Method for measuring main stream smoke of cigarettes by using amino acid analyzer
The invention discloses a method for measuring main stream smoke of cigarettes by using an amino acid analyzer. The analytical method which is used for measuring ammonia in the main stream smoke of cigarettes by using the amino acid analyzer and a post column derivation device thereof is established according to the selection specificity of color development reaction of ammonia and ninhydrin and by using the design principle of the amino acid analyzer and improving an amino acid analysis method, and the problem of sodion interference puzzled by ion chromatography is successfully avoided; and the method disclosed by the invention is simple, convenient, rapid and accurate and is suitable for actual analysis and detection of a cigarette smoke sample.
Owner:GUIZHOU TOBACCO SCI INST
Method for simultaneously determining active peptide and free amino acid
ActiveCN106468695ARapid determinationEfficient determinationComponent separationElutionTime-Consuming
The invention belongs to the field of analytical chemistry, which relates to a method for determining nutrients, and discloses a method for simultaneously determining active peptide and free amino acid. By adjusting the composition of a buffer, elution process and elution temperature during an amino acid analysis determinator standard determination process, a plurality of amino acid peaks and active peptide peaks are separated, and an amino acid automatic analyzer can perform one-step determination on the free amino acid and the active peptide, a method for preparing the amino acid and peptide simultaneously is established, and the disadvantages that the active peptide and a plurality of free amino acids cannot be separated, and the time consuming for testing is long by a traditional amino acid analyzer method are overcome.
Owner:SHANGHAI OCEAN UNIV
Method for analysis and determination by measuring content of amino acids and proteins in food and feed
The invention discloses a method for analysis and determination by measuring the content of amino acids and proteins in food and feed. The content of proteins is measured by a kjeldah method and the content of various free and hydrolytic amino acids and the total content of amino acids are measured by an amino acid analyzer; a lot of samples are analyzed fundamentally to form a database and a judgment system; and each measured result is analyzed and compared with system data to judge whether fake proteins are adulterated in the food and the feed. By the method, a precise judgment can be made that components of non-proteins or fake proteins are adulterated in the sample only if one or more of the following measured data is abnormal: content of non-proteins, content of free amino acids, content of characteristic amino acids, percentage between the total content of amino acids and the proteins, the ratio of each component of the amino acids to the total content of the amino acids and the like, so that the quality safety of the food and the feed are ensured.
Owner:GUANGDONG FOOD IND INST
Method for measuring lysine in corn through near infrared spectrum
InactiveCN102175620ARapid determinationNon-destructive determinationColor/spectral properties measurementsMethod selectionLysine
The invention relates to a method for measuring lysine in corn through a near infrared spectrum. The method comprises the following steps of: testing a spectrum, rejecting an abnormal value, establishing an analysis model and the like. By the method, the lysine content can be rapidly and nondestructively measured without pretreatment, and the method is particularly suitable for measuring the lysine content of low-generation materials in the process of breeding high-quality protein corn; compared with lysine content measured results in a dye binding method (DBL), a ninhydrin development process and an amino acid analyzer method, the lysine content measured result in the method verifies the feasibility of a method for measuring the corn through the near infrared spectrum, particularly lysine of the low-generation materials in the process of breeding the high-quality protein corn; moreover, the method is rapid, reliable and practical, and has certain reference value for selecting a method for measuring the lysine content in the process of breeding the high-quality protein corn.
Owner:HENAN ACAD OF AGRI SCI
Method for using amino acid analyzer to determine tranexamic acid content
ActiveCN109959736AHigh measurement sensitivityHigh degree of automationComponent separationInjection volumeIon exchange
The invention provides a method for using an amino acid analyzer to determine the tranexamic acid content. The method for determining the tranexamic acid content through ion exchange chromatography and a standard curve method is used. The chromatographic conditions are a 4-micron prewash column and an 8-micron analytical column. Ion exchange resin is resin dedicated to the Biochrom30+ high efficiency amino acid automatic analyzer. The detection wavelength is 570nm. The pump flow rate is 35ml / h. A gradient elution procedure comprises a 0 to 1min buffer 1, a 1 to 2min buffer 2, a 2 to 20min buffer 5, a 20 to 21.5min buffer 6 and a 21.5 to 23.5min buffer 1. The column temperature is 44 DEG C. The reaction temperature is 135 DEG C. The injection volume is 20 mu l. The amino acid analyzer usedfor determining the tranexamic acid content has the characteristics of high commercialization and automation. The method for determining the tranexamic acid content by using the amino acid analyzer has the advantages of simple operation, strong standardization, high sensitivity, being fast and efficient and the like.
Owner:BEIJING INST FOR DRUG CONTROL
Method for glucosamine determination by amino acid analyzer
ActiveCN105738527AGood physical propertiesComponent separationQuantitative determinationChromatographic column
The invention relates to a method for glucosamine determination by an amino acid analyzer.The method can be used for qualitative and quantitative determination of glucosamine and analogues thereof in foods, healthcare foods, medicines or raw materials thereof.The method includes steps: providing the amino acid analyzer, a cation exchange resin chromatographic column and a mobile phase for elution chromatography, preparing a test solution, a reference solution and the like according to specified chromatographic analysis conditions, and testing related test objects.By implementation of the method for glucosamine determination by the amino acid analyzer, a glucosamine qualitative and quantitative analysis method which is efficient, rapid, sensitive and accurate is established completely to lay a good foundation for detection and application of the glucosamine to foods, healthcare foods and medicines.
Owner:BEIJING INST FOR DRUG CONTROL
Method for quantitatively detecting sulfur-containing amino acid in sludge
The invention discloses a method for quantitatively detecting sulfur-containing amino acid in sludge. The method comprises the following steps: cold-drying, grinding and sieving the sludge, so as to prepare a sample; oxidizing sulfur-containing amino acid in the sludge into cysteic acid and methionine sulfone; hydrolyzing protein by virtue of concentrated hydrochloric acid, and carrying out ultrafiltration, edulcoration and purification on hydrolysate by virtue of 3kD filter membrane; processing by virtue of o-phthalaldehyde as a derivating agent, so as to enable a sulfur-containing amino acid derivative to have fluorescence characteristics; and finally, carrying out quantitative analysis on the derivative by virtue of a high performance liquid chromatography. Compared with a conventional method, the method has the advantages that the operation is simple and convenient, the toxicity is low, an expensive amino acid analysis meter does not need to be used, the detection accuracy and the sensitivity are high, the repeatability is good, and the like.
Owner:SUZHOU RES INST OF TONGJI UNIV +2
Method for characterizing characteristic taste of steamed fish sample and application
InactiveCN111289707AThe method is objective and accurateSensory evaluation is objective and accurateComponent separationPreparing sample for investigationBiotechnologyAnimal science
The invention belongs to the technical field of analysis of non-volatile flavor substances of steamed fish, and discloses a method for characterizing the characteristic taste of a steamed fish sampleand application. The method comprises the following steps of: selecting one piece of sturgeon back meat as a fresh sample, steaming the rest five pieces of sturgeon back meat by a common induction cooker, boiling water, then putting the sturgeon back meat into a steamer, starting timing, and respectively conducting steaming for 4-26 minutes; analyzing the non-volatile components of the steamed fish at different time by an electronic tongue technology, and conducting quantifying by a high performance liquid chromatograph, an atomic absorption spectrophotometer and an amino acid analyzer, and evaluating the change rule of the non-volatile flavor components of the fish at different steaming time. Compared with single sensory evaluation, the method is more objective and accurate, the change rule of the non-volatile flavor components of the fish at different steaming time can be evaluated through quantification of the non-volatile substances, and actual theoretical reference is provided forclarification of the non-volatile components of the steamed fish and reasonable selection of cooking time.
Owner:OCEAN UNIV OF CHINA
Method for determination of free amino acid in Coptis chinensis
The invention provides a method for determination of free amino acid in Coptis chinensis. The method is ion exchange chromatography determination based on cation exchange column separation, post-column ninhydrin derivatization, and spectrophotometric determination. Specifically, to-be-determined Coptis chinensis is added into an amino acid analyzer, then the mobile phase B1-B6 is employed for gradient elution. The preparation method of the to-be-determined Coptis chinensis includes: taking Coptis chinensis powder, adding water for extraction, adding a sulfosalicylic acid aqueous solution witha mass fraction of 5%, and conducting centrifugation and filtration. The method provided by the invention is accurate and reliable, simple and fast, has high sensitivity and good stability, also is low in cost, and provides detection basis for development and utilization, and medicinal value study of Coptis chinensis.
Owner:CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
A kind of culture method that can improve mycosporine-like amino acid content in umbilical laver
ActiveCN105684880BIncrease productionThe production of MAAs did not change significantly with the increase of photoperiodSaving energy measuresCultivating equipmentsBiotechnologyUltraviolet lights
The invention relates to a culture method for improving the mycosporine-like amino acid content in porphyra umbilicalis. The method is characterized by including the following steps that 1, the vigorously growing porphyra umbilicalis is collected, screened and cleaned, is subjected to irradiation with ultraviolet rays with the wavelength of 254 nm for 3-10 minutes for sterilization and serves as a culture object; 2, the porphyra umbilicalis obtained in the step 1 is put in a piping type algae cultivation pool for seawater culture, the temperature is controlled to 20-35 DEG C, the pH is controlled to 5.6-6.4, multiple light sources consisting of an ultraviolet light lamp, a purple light lamp, a green light lamp and a yellow light lamp is adopted to perform continuous irradiation culture for 3-5 cycles, air is pumped in the culture process to keep the porphyra umbilicalis in a suspended state, possibly existing hybrid algae is periodically observed and removed to make the porphyra umbilicalis grow continuously, collection and air-drying are performed after the length of upright branches reaches 4-5 cm, and an amino acid analyzer and UHPLC are adopted to detect the MAAs content of the dried porphyra umbilicalis. The culture method is low in production cost, and the MAAs content of the cultured porphyra umbilicalis is increased remarkably.
Owner:PROYA COSMETICS
Rapid detection method for taurine content in marine product processing process
InactiveCN105044019AHigh precisionShort analysis timeComponent separationMaterial analysis by optical meansProduct processingReagent
The invention relates to a rapid detection method for taruine content in a marine product processing process. The rapid detection method is characterized by comprising the following steps: (1) collecting extracted liquid samples in the various stages in the taurine extracting process, wherein each sample is divided into two parts, the near infrared spectrum of one sample is measured, and the taurine content of the other sample is measured by an amino acid analyzer; (2) detecting the near infrared spectrums of the samples; (3) measuring the taurine contents of the samples; (4) establishing a regression prediction model; and (5) predicting the taurine contents of the samples by using the regression prediction model. According to the rapid detection method, the near infrared spectrum of the extracted liquid is measured, the taruine content of the extracted liquid can be rapidly obtained by utilizing an established calibration model; compared with the conventional amino acid analysis method, the rapid detection method has the advantages that the analysis time is greatly shortened, the amount of the reagent is saved, and the precision of the predication result is high.
Owner:ZHEJIANG WANLI UNIV
Free amino acid analysis method for identifying fermented type and formulated type yogurt beverages
ActiveCN104950046AResolve indistinguishablePhysical avoidanceComponent separationSulfosalicylic acidAmino acid content
The invention discloses a method for identifying fermented type and formulated type yogurt beverages. Yogurt beverage is precipitated by sulfosalicylic acid, the content of free amino acids in liquid supernatant is measured by an amino acid analyzer, and the content ratios of four characteristic amino acids-alanine, valine, lysine and proline of total free amino acids are judged to establish the amino acid analysis method for identifying fermented type and formulated type yogurt beverages. According to the method, the similarity of the fermented type and formulated type yogurt beverages in the aspects of mouthfeel and physical behavior is avoided, the difference of the contents of the free amino acids of the fermented type and formulated type yogurt beverages is fully utilized, and a problem that the fermented type and formulated type yogurt beverages cannot be rapidly identified is solved; and the identification method can be operated simply and conveniently and has high accuracy and good application prospect.
Owner:ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Pretreatment method for fresh food materials and method for determining amino acid content
The invention discloses a pretreatment method for fresh food materials and a method for determining amino acid content. The pretreatment method for the fresh food materials uses microwave digestion for acid hydrolysis of the fresh food materials and comprises the following steps that (a) a sample is extracted; (b) microwave-hydrolysis is conducted; (c) hydrolysate concentration is roughly measured; and (d) adetermining solution is prepared. The invention further discloses the method for determining the amino acid content of the fresh food materials.The method for determining the amino acid content of the fresh food materialsis derived from the determining solution prepared in the pretreatment methodfor the fresh food materials, usesan full-automatic amino acid analyzer for measuring the amino acid content, and comprises the following steps that (1) standard machinefluidis configured; (2) standard machine fluid is determined; (3) the amino acid content in fresh food materials is determined. According to the pretreatment method for the fresh food materials and the method for determining the amino acid content, the pretreatment process is simple, the hydrolysis speed of the sample ishigh, and the advantages of safe operation and pollution-free are obtained.
Owner:广东省中鼎检测技术有限公司
Method for Determination of Tranexamic Acid Content Using Amino Acid Analyzer
ActiveCN109959736BHigh measurement sensitivityHigh degree of automationComponent separationPhysical chemistryIon exchange
Disclosed is a method for determining the content of tranexamic acid using an amino acid analyzer, and a method for determining the content of tranexamic acid by using ion-exchange chromatographic separation and a standard curve method. The chromatographic conditions are 4μm prewash column and 8μm analysis column, and the ion exchange resin is Biochrom30+ special resin for high-efficiency amino acid automatic analyzer; detection wavelength is 570nm; pump flow rate is 35ml / h; gradient elution program: 0~1min buffer 1, 1~ 2min buffer 2, 2-20min buffer 5, 20-21.5min buffer 6, 21.5-23.5min buffer 1; column temperature 44°C; reaction temperature 135°C; injection volume 20μl. The amino acid analyzer used in the determination of tranexamic acid content has the characteristics of commercialization and high degree of automation. The method for determining the content of tranexamic acid with an amino acid analyzer has the advantages of simple operation, strong standardization, high sensitivity, rapidity and high efficiency.
Owner:BEIJING INST FOR DRUG CONTROL
Method for Determination of Free Ammonia in Compound Amino Acid Injection
The invention discloses a method for measuring free ammonia in compound amino acid injection, the method comprising the following steps: providing an amino acid analyzer and a chromatographic column; preparing a buffer solution for separation and analysis; preparing a derivatization reaction reagent; the flow rate of the mobile phase is 0.40 ml / min, the flow rate of the derivatizing reagent is 0.35ml / min, the column temperature is 57°C, the reactor temperature is 135°C, the detection wavelength is 570nm, the injection volume is 5μl, and the gradient is eluted; the preparation of the test solution; the preparation of the reference solution Formulation and chromatographic determination. Wherein, during chromatographic determination, calculate the concentration of free ammonia in the test solution with the peak area according to the external standard method, and calculate the content of free ammonia in the compound amino acid injection according to the preparation method of the test solution. It also relates to the compound amino acid injection determined by the method of the invention. The method of the present invention exhibits excellent technical effects as described in the description, such as rapidity, simplicity, sensitivity and stability.
Owner:BEIJING INST FOR DRUG CONTROL
Method for rapidly and qualitatively detecting chemical components in marinated meat soup
PendingCN112362611AEasy to operateRapid Qualitative DetectionMaterial analysis by optical meansBiotechnologyGas liquid chromatographic
The invention provides a method for rapidly and qualitatively detecting chemical components in marinated meat soup, which belongs to the field of food detection. According to the method, after the to-be-detected marinated meat soup sample containing a large amount of water is subjected to freeze drying for the first time, Fourier mid-infrared detection is performed on the premise of not damaging the original chemical composition and component content of the to-be-detected marinated meat soup sample, so that the method is simple and easy to operate, is more suitable for large-scale detection, and can realize rapid qualitative detection of chemical components in the marinated meat soup. The method does not need to use chemical reagents, avoids the problems of consumption of a large amount ofchemical reagents, increase of the detection cost and environmental pollution in the existing detection technology, and has the advantages of simple operation of the detection process, very small amount of required samples, simple operation of the Fourier transform mid-infrared spectrometer, and easy operation of operators, and the problems of high requirements of the existing detection technology on operators, such as determination of fatty acid by gas chromatography, determination of nucleotide by high performance liquid chromatography and determination of amino acid by an amino acid analyzer, are avoided.
Owner:SHANGHAI OCEAN UNIV
Method for establishing konjak fingerprint pattern
Owner:ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
Method for authenticity identification of Maca powder and determination of Maca content
InactiveCN106324127BEasy to handleMeet testing needsComponent separationContent determinationAminoacid analyzer
The invention relates to a method for utilizing free amino acid spectroscopic data for identifying the authenticity of a maca product and determining the content of raw maca in the maca product. The method comprises the main steps of 1, manufacturing a mixed maca product; 2, manufacturing a detection sample; 3, adopting an amino acid analyzer for determining free amino acid spectroscopic data in pure maca, fake maca and mixed maca; 4, analyzing and processing the free amino acid data. According to the method, preprocessing is easy and convenient, maca authenticity identification and product maca content determination can be achieved rapidly, and the method is convenient to implement and practical. The prediction error of the method is smaller than 10%, and the detection requirement for the maca product can be better met. At present, no determination method for the content of maca in the maca product exists in China, and the method can be used for quality control in the industrial field of maca and products thereof.
Owner:INST OF QUALITY STANDARD & DETECTION TECH YUNNAN ACAD OF AGRI SCI
A method for simultaneous determination of active peptides and free amino acids
ActiveCN106468695BRapid determinationEfficient determinationComponent separationElutionTime-Consuming
The invention belongs to the field of analytical chemistry, which relates to a method for determining nutrients, and discloses a method for simultaneously determining active peptide and free amino acid. By adjusting the composition of a buffer, elution process and elution temperature during an amino acid analysis determinator standard determination process, a plurality of amino acid peaks and active peptide peaks are separated, and an amino acid automatic analyzer can perform one-step determination on the free amino acid and the active peptide, a method for preparing the amino acid and peptide simultaneously is established, and the disadvantages that the active peptide and a plurality of free amino acids cannot be separated, and the time consuming for testing is long by a traditional amino acid analyzer method are overcome.
Owner:SHANGHAI OCEAN UNIV
Fast plate method for screening of methionine producing bacteria
InactiveCN110241169ALow detection limitReduce absorptionMicrobiological testing/measurementBiotechnologyMicrobiology
The invention relates to a method for screening of methionine producing bacteria. A method in the prior art usually comprises the steps of transferring single colonies appearing on a flat into a shake flask, performing culturing for some time, and analyzing whether methionine is contained or not in fermentation liquid by methods of a spectroscopical photometric method, a chromatography or amino acid analyzer and the like. The method in the prior art is time-consuming. According to the method provided by the invention, the technological processes are simplified; after the colonies grow in plates and are cultured for some time, a series of sodium nitroprusside reagents are directly added to the surfaces of the colonies according to quantity, and after a reaction, whether the color is red is observed, so as to determine whether the colonies can produce methionine. According to the method disclosed by the invention, the screening time for methionine producing bacteria is shortened, and the screening efficiency is improved.
Owner:南京先华生物科技有限公司
Method for detecting effective ingredients in deer antler wine
InactiveCN105372189AStrong reductionClear qualityMaterial analysis by observing effect on chemical indicatorComponent separationNutritive valuesDichlorophenolindophenol
The invention relates to a method for detecting effective ingredients in deer antler wine. According to the method, with the deer antler wine as an experimental material, protein, saccharides, lipids, Vc content, diethylstilbestrol and amino acid in the deer antler wine are sequentially analyzed and determined through a full-automatic amino acid analyzer by using a Coomassie brilliant blue colorimetric method, a potassium ferricyanide titration method, a diethyl ether extraction method, a 2,6-dichlorindophenol titration method and a high performance liquid chromatography method. Nutritional ingredients contained in the deer antler wine are clearly learned about through final experimental data analysis so that the nutritive value of the deer antler wine can be better interpreted.
Owner:LIAONING UNIVERSITY
A free amino acid analysis method for the identification of fermented and blended yogurt drinks
ActiveCN104950046BResolve indistinguishableEasy to operateComponent separationSulfosalicylic acidAmino acid content
The invention discloses a method for identifying fermented type and formulated type yogurt beverages. Yogurt beverage is precipitated by sulfosalicylic acid, the content of free amino acids in liquid supernatant is measured by an amino acid analyzer, and the content ratios of four characteristic amino acids-alanine, valine, lysine and proline of total free amino acids are judged to establish the amino acid analysis method for identifying fermented type and formulated type yogurt beverages. According to the method, the similarity of the fermented type and formulated type yogurt beverages in the aspects of mouthfeel and physical behavior is avoided, the difference of the contents of the free amino acids of the fermented type and formulated type yogurt beverages is fully utilized, and a problem that the fermented type and formulated type yogurt beverages cannot be rapidly identified is solved; and the identification method can be operated simply and conveniently and has high accuracy and good application prospect.
Owner:ZHEJIANG UNIVERSITY OF SCIENCE AND TECHNOLOGY
Method for measuring main stream smoke of cigarettes by using amino acid analyzer
The invention discloses a method for measuring main stream smoke of cigarettes by using an amino acid analyzer. The analytical method which is used for measuring ammonia in the main stream smoke of cigarettes by using the amino acid analyzer and a post column derivation device thereof is established according to the selection specificity of color development reaction of ammonia and ninhydrin and by using the design principle of the amino acid analyzer and improving an amino acid analysis method, and the problem of sodion interference puzzled by ion chromatography is successfully avoided; and the method disclosed by the invention is simple, convenient, rapid and accurate and is suitable for actual analysis and detection of a cigarette smoke sample.
Owner:GUIZHOU TOBACCO SCI INST
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