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Method for glucosamine determination by amino acid analyzer

A technology for glucosamine and amino acids, which is applied to analytical materials, instruments, and measuring devices, and can solve problems such as complex operations, low sensitivity, and low specificity

Active Publication Date: 2016-07-06
BEIJING INST FOR DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When there are many compound components in food, health food and medicine, the above method has various bottleneck problems, such as complicated operation, many influencing factors, weak specificity, low sensitivity, or difficult to promote, etc.

Method used

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  • Method for glucosamine determination by amino acid analyzer
  • Method for glucosamine determination by amino acid analyzer
  • Method for glucosamine determination by amino acid analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, use amino acid analyzer to measure the glucosamine in the product

[0035] Mobile phase is pH3.20 citric acid / sodium citrate buffer (citrate concentration 0.05M, all refers to 0.05M when not providing its concentration in the example below), amino acid analyzer pump flow rate is 35ml / h, chromatographic column temperature The reaction temperature is 45°C, the reaction temperature is 135°C, the analysis time is 50min, the detection wavelength is 570nm, and the injection volume is 20μl. The cation exchange resin column has a length of 20 cm and a diameter of 5 mm. The following examples are not otherwise specified, and the specifications of the chromatographic column are the same.

[0036] Preparation of the test solution: Take the test product equivalent to 20mg of glucosamine, accurately weigh it, place it in a 250ml volumetric flask, add 150ml of water to dilute it, extract it ultrasonically for 15min, set the volume to the mark after cooling, and pass t...

Embodiment 2

[0040] Embodiment 2, use amino acid analyzer to measure the glucosamine in the product

[0041] The mobile phase is pH 4.25 citric acid / sodium citrate buffer (citrate concentration 0.025M), the pump flow rate of the amino acid analyzer is 35ml / h, the column temperature is 60°C, the reaction temperature is 120°C, and the analysis time is 40min , the detection wavelength is 570nm, and the injection volume is 20μl.

[0042] Preparation of the test solution: take the test product equivalent to 50mg of glucosamine, accurately weigh it, place it in a 250ml volumetric flask, add 150ml of water to dilute, extract it by ultrasonic for 15min, set the volume to the mark after cooling, and pass through a micropore of 0.45μm Membrane filtration, continued filtrate as the test solution.

[0043] Preparation of reference substance solution: take 20 mg of glucosamine hydrochloride reference substance, accurately weigh it, place it in a 10ml volumetric flask, dilute with water and set the v...

Embodiment 3

[0046] Embodiment 3, use amino acid analyzer to measure the glucosamine in the product

[0047] The mobile phase is pH 6.45 citric acid / sodium citrate buffer (citrate concentration 0.03M), the pump flow rate of the amino acid analyzer is 35ml / h, the column temperature is 80°C, the reaction temperature is 130°C, and the analysis time is 30min , the detection wavelength is 570nm, and the injection volume is 20μl.

[0048] Preparation of the test solution: take the test product equivalent to 100mg of glucosamine, accurately weigh it, place it in a 250ml volumetric flask, add 150ml of water to dilute it, extract it ultrasonically for 15min, set the volume to the mark after cooling, and pass through a micropore of 0.45μm Membrane filtration, continued filtrate as the test solution.

[0049] Preparation of reference substance solution: take 20 mg of glucosamine hydrochloride reference substance, accurately weigh it, place it in a 10ml volumetric flask, dilute with water and set t...

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Abstract

The invention relates to a method for glucosamine determination by an amino acid analyzer.The method can be used for qualitative and quantitative determination of glucosamine and analogues thereof in foods, healthcare foods, medicines or raw materials thereof.The method includes steps: providing the amino acid analyzer, a cation exchange resin chromatographic column and a mobile phase for elution chromatography, preparing a test solution, a reference solution and the like according to specified chromatographic analysis conditions, and testing related test objects.By implementation of the method for glucosamine determination by the amino acid analyzer, a glucosamine qualitative and quantitative analysis method which is efficient, rapid, sensitive and accurate is established completely to lay a good foundation for detection and application of the glucosamine to foods, healthcare foods and medicines.

Description

technical field [0001] The invention relates to a qualitative and quantitative determination method for glucosamine and its analogs in food, health food, medicine or raw materials thereof. Background technique [0002] Glucosamine is a substance that can be synthesized in the human body. It is an important nutrient for the formation of chondrocytes, a natural tissue component of healthy articular cartilage, and an essential substrate for chondrocyte biosynthesis and metabolism. As we grow older, the lack of glucosamine in the human body becomes more and more serious, leading to bone and joint diseases and osteoarthritis. A large number of medical studies have shown that: glucosamine can help repair and maintain cartilage, and can stimulate the growth of chondrocytes, improve joint mobility, and relieve joint pain. It has a good curative effect on the treatment of rheumatoid arthritis, and is the main raw material for the synthesis of antibiotics and anticancer drugs, and ca...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 孙亮张华珺张蓉邬国庆邸铮刘齐贾伯阳韩萧茜
Owner BEIJING INST FOR DRUG CONTROL
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