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Method for detecting sulfur-containing amino acid

A detection method and amino acid technology, applied in the field of chemical analysis and detection, can solve the problems of low penetration rate, high cost and high cost of sulfur-containing amino acids, and achieve the effects of low detection cost, high sensitivity and high accuracy

Active Publication Date: 2013-08-28
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Problems solved by technology

Due to the strong specificity and high cost of amino acid analyzers, the cost of sulfur-containing amino acid detection is high, so the penetration rate of detection is low

Method used

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  • Method for detecting sulfur-containing amino acid
  • Method for detecting sulfur-containing amino acid
  • Method for detecting sulfur-containing amino acid

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Experimental program
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specific Embodiment approach

[0019] 1. Instruments and reagents

[0020] UV1201 ultraviolet-visible detector, P1201 high-pressure constant-flow pump, Elite AAK, 5μm, 4.6×250mm chromatographic column, ZW II chromatographic column thermostat, EC2006 chromatographic workstation (the above are all products of Dalian Elite Analytical Instrument Co., Ltd.), oven , Alcohol lamp, ampoules, electric heating constant temperature water bath, nitrogen blowing instrument.

[0021] Methionine standard product (Beijing Aoboxing Biotechnology Co., Ltd.), cystine standard product (Shanghai Zhengxiang Chemical Reagent Research Institute), feed sample, 30% hydrogen peroxide, 88% formic acid, hydrochloric acid (excellent grade), heavy weight Sodium sulfite, Elite-AAK Amino Acid Analysis System Kit, deionized water, etc. (all are of analytical grade unless otherwise noted).

[0022] 2. Detection method

[0023] 2.1 Method principle

[0024] The methionine in the feed is oxidized with formic acid, and then converted into me...

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Abstract

The invention discloses a method for detecting sulfur-containing amino acid. The method comprises the following steps of: oxidizing cystine and methionine and preparing cysteic acid and metson by acid hydrolysis; adding a derivatization reagent of 2,4-dinitrofluorobenzene to the cysteic acid and the metson for derivatization reaction to generate products having the function of ultraviolet absorption; and performing highly efficient liquid phase chromatographic analysis of the derivatives. The method has the advantages that: the amino acid analyzer with high price is unnecessary; the operationis simple; the detection cost is low; and the accuracy and the sensitivity are high.

Description

Technical field: [0001] The invention belongs to the field of chemical analysis and detection, in particular to a detection method for sulfur-containing amino acids with simple operation, low detection cost, high accuracy and high sensitivity. Background technique: [0002] Sulfur-containing amino acids usually refer to methionine, cystine and cysteine, which play a vital role in the metabolism of organisms. Methionine is a sulfur-containing essential amino acid, which is closely related to the metabolism of various sulfur-containing compounds in the organism. When the organism lacks methionine, it will cause loss of appetite, slow growth or no weight gain, enlarged kidneys, and iron accumulation in the liver. Lead to liver necrosis or fibrosis; methionine can also use its methyl group to methylate toxic substances or drugs to detoxify. Therefore, at present, methionine can be used to prevent and treat liver diseases such as chronic or acute hepatitis and cirrhosis, and can...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36G01N30/74
Inventor 于淑新冯思韩晶孙元社唐涛李彤
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