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Method for determination of free amino acid in Coptis chinensis

A technology of free amino acid and Coptis chinensis, which is applied in measuring devices, material separation, instruments, etc., can solve problems such as the inability to determine the type of amino acid and the inability to meet work needs

Pending Publication Date: 2018-01-26
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, when using spectrophotometry to measure amino acids in Coptis Rhizoma, it can only solve the analysis of the total amount of amino acids, but cannot determine the types of amino acids, which cannot meet the needs of actual work.

Method used

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  • Method for determination of free amino acid in Coptis chinensis
  • Method for determination of free amino acid in Coptis chinensis
  • Method for determination of free amino acid in Coptis chinensis

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Embodiment 1

[0037] Embodiment 1 The inventive method measures free amino acid in Coptis Rhizoma

[0038] 1. Solution preparation and experimental equipment

[0039] 1. Standard solution configuration

[0040] 1.1 Mixed amino acid standard stock solution configuration

[0041] The amino acid mixed standard solution contains 17 commonly used amino acids and ammonium chloride formed by the hydrolysis of glutamic acid and glutamine; respectively: L-aspartic acid (L-Asp); L-threonine (L- Thr); L-serine (L-Ser); L-glutamic acid (L-Glu); L-proline (L-Pro); Glycine (Gly); L-alanine (L-Ala); L-cystine (L-Cys); L-valine (L-Val); L-methionine (L-Met); L-isoleucine (L-Ile); L-leucine (L-Ile) -Leu); L-tyrosine (L-Tyr); L-phenylalanine (L-Phe); L-lysine (L-Lys); L-histidine (L-His); - Arginine (L-Arg); Ammonium Chloride. The concentration of each amino acid is 2.50μmol / mL, dissolved in about 0.1mol / mL hydrochloric acid solution.

[0042] In addition, weigh an appropriate amount of glutamine (Gln)...

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Abstract

The invention provides a method for determination of free amino acid in Coptis chinensis. The method is ion exchange chromatography determination based on cation exchange column separation, post-column ninhydrin derivatization, and spectrophotometric determination. Specifically, to-be-determined Coptis chinensis is added into an amino acid analyzer, then the mobile phase B1-B6 is employed for gradient elution. The preparation method of the to-be-determined Coptis chinensis includes: taking Coptis chinensis powder, adding water for extraction, adding a sulfosalicylic acid aqueous solution witha mass fraction of 5%, and conducting centrifugation and filtration. The method provided by the invention is accurate and reliable, simple and fast, has high sensitivity and good stability, also is low in cost, and provides detection basis for development and utilization, and medicinal value study of Coptis chinensis.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine analysis, and relates to a method for measuring free amino acids in Coptidis rhizome. Background technique [0002] Amino acid is the basic unit of protein and the material basis of life metabolism. It is widely found in food, medicine and medicinal plants. Various amino acids in Chinese medicine are the main components of treating diseases or nourishing nutrition, and are also an important factor to measure the quality of Chinese medicinal materials. index. The analysis and detection of free amino acids in Chinese medicinal materials has very important guiding significance for their medicinal value research, quality identification and related product development. [0003] Coptis chinensis is the dry rhizome of Coptis chinensis Franch., Coptis deltoidea C.Y. Chenget Hsiao or Coptis deltoidea C.Y. Chenget Hsiao or Coptis chinensis wall. of the Ranunculaceae plant. Mainly produced in Si...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 何洋李东梅陈涵婷
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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