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Method for quantitatively detecting sulfur-containing amino acid in sludge

A quantitative detection, amino acid technology, applied in the field of chemical analysis and detection, can solve the problems of low penetration rate, no report on detection, high detection cost, and achieve the effects of low detection cost, low toxicity and high sensitivity

Active Publication Date: 2017-03-08
TONGJI UNIV
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Problems solved by technology

However, due to the strong specificity and high detection cost of amino acid analyzers, the popularity of amino acid analyzers is low.
At present, the detection of sulfur-containing amino acids is mainly concentrated in the fields of food and medicine, and the detection of sulfur-containing amino acids in complex environmental samples, such as sewage and sludge, has not been reported yet.

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  • Method for quantitatively detecting sulfur-containing amino acid in sludge
  • Method for quantitatively detecting sulfur-containing amino acid in sludge
  • Method for quantitatively detecting sulfur-containing amino acid in sludge

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Embodiment １

[0023] 1. Instruments and reagents

[0024] Agilent 1260 G1321B FLD fluorescence detector, Agilent 1260 G1311C quaternary pump, Agilent 1260 G1329B standard autosampler, Agilent 1260 G1316A column thermostat, Agilent ZORBAX Eclipse AAA 3.5μm×4.6mm×150mm, oven, hydrolysis tube, electric thermostat Water bath, 3K Millipore ultrafiltration centrifuge tube, high-speed centrifuge, rotary evaporator, sample bottle, inner tube.

[0025] Cysteine ​​standard, methionine standard, sulfoalanine standard and methionine sulfone standard (Shanghai Anpu Experimental Technology Co., Ltd.), dewatered sludge (Shanghai Jiading Wastewater Treatment Plant), 30% hydrogen peroxide , 88% performic acid, hydrochloric acid (excellent grade), sodium metabisulfite, boric acid, phosphoric acid (HPLC grade), o-phthalaldehyde (HPLC grade), mercaptopropionic acid (HPLC grade), sodium dihydrogen phosphate dihydrate (HPLC grade ), etc. (unless otherwise specified, all are analytically pure).

[0026] 2. Dete...

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Abstract

The invention discloses a method for quantitatively detecting sulfur-containing amino acid in sludge. The method includes: performing freezing drying, grinding and sieving on the sludge to obtain a sample; using formic acid to convert the sulfur-containing amino acid in the sludge into cysteic acid and methionine sulfone; using concentrated hydrochloric acid to hydrolyze protein, and using a 3kD filter membrane to perform ultrafiltration, immunity removing and purification on hydrolysate; using phthalic dicarboxaldehyde as the derivative agent to allow the sulfur-containing amino acid derivatives to have fluorescent characteristics; using high-performance liquid chromatography to perform quantitative analysis on the derivatives. Compared with a conventional method, the method has the advantages that the method is simple and convenient to operation, low in toxicity, high in detection accuracy, high in sensitivity and good in repeatability, an expansive amino acid analyzer is not needed, and the like.

Description

technical field [0001] The invention belongs to the field of chemical analysis and detection, and is a method for quantitatively detecting sulfur-containing amino acids in sludge with relatively simple operation, low toxicity, low cost, high accuracy and high sensitivity. Background technique [0002] In recent years, the odor problem caused by sewage treatment plants has attracted more and more attention. Among them, sulfur-containing substances play an important role in causing the generation of bad odor. In sewage treatment plants, volatile organic sulfur (VOSCs), such as methyl mercaptan (MM), dimethyl sulfide (DMS), etc., and inorganic sulfur, such as hydrogen sulfide (H 2 S) etc. have been identified as the most important substances causing bad odor. The odor threshold of this kind of substance is extremely low, and the stench it produces is very serious, even trace amounts of VOCS and H 2 S can also make a great contribution to odor pollution. Studies have shown t...

Claims

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Application Information

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IPC IPC(8): G01N30/06
CPCG01N30/06G01N2030/067
Inventor 董滨熊南安卢怡清王有晴于晓庆夏兆辉孟金凤戴晓虎
Owner TONGJI UNIV
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