Method for establishing konjak fingerprint pattern
A technology of fingerprints and establishment methods, which is applied in the field of establishment of konjac fingerprints, and can solve the problems that chemical fingerprints have not yet been reported.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] (a) Preparation of samples: take normal A. konjac (Normal A. Konjac), soft rot diseased A. konjac (Erwinia carotvora A. Konjac), white A. albus (Normal A. bulbifer) leaves Finish at 115°C for 10 minutes, dry at 80°C, grind, weigh 0.5000g into a test tube, add 10mL of pure water, keep it in a drying oven at 80°C for 12 hours, take it out, dilute to 10mL with pure water, filter with filter paper , the solution was filtered with a 0.45 μM filter membrane, and the water-soluble amino acid content was determined by the AccQ.Tag method;
[0026] (b) Chromatographic conditions: use American Waters 2695 separation unit + 2996 diode matrix detector; Milli-Q ultrapure water device (Milli-Q Company in the United States); KQ-50B ultrasonic cleaner; vacuum drying oven; 17 kinds of amino acid standard samples (provided by Waters Company); acetonitrile (chromatographically pure, Tianjin Fuchen), methanol (chromatographically pure, Tianjin Fuchen), sodium acetate-triethylamine buffer s...
Embodiment 2
[0029] Its production steps are:
[0030] (a) Preparation of sample: take konjac leaves and fix them at 115°C for 10 minutes, dry them at 80°C, grind them, weigh 0.5000g into a test tube, add 10mL of pure water, keep them overnight in a drying oven at 80°C, take them out, and use Dilute the purified water to 10 mL, filter the solution with a 0.45 μm filter membrane, and use the AQC method introduced by Waters in 1994, that is, accurately draw 10 μl of the test sample into the derivation tube, add 70 μl of buffer solution, and then vortex Add 10 μl derivative agent while stirring on the stirrer, mix well, keep it in a 60°C oven for 10 minutes, take it out, transfer it to a sample injection vial, and wait for the test;
[0031] (b) Chromatographic conditions: the instrument is American Waters 2695 separation unit + 2996 diode matrix detector, mobile phase A is 140mM sodium acetate-triethylamine buffer solution (pH4.95), mobile phase B: 100% acetonitrile, mobile phase C : 100% u...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com