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Method for establishing konjak fingerprint pattern

A technology of fingerprint spectrum and establishment method, which is applied in the field of establishment of konjac fingerprint spectrum, and can solve the problem that chemical fingerprint spectrum has not yet been reported.

Inactive Publication Date: 2008-08-27
ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, fingerprints have become internationally recognized as the most effective means to control the quality of traditional Chinese medicines or natural medicines, but the research on chemical fingerprints of konjac has not yet been reported.

Method used

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  • Method for establishing konjak fingerprint pattern

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (a) Preparation of samples: take normal A. konjac (Normal A. Konjac), soft rot diseased A. konjac (Erwinia carotvora A. Konjac), white A. albus (Normal A. bulbifer) leaves Finish at 115°C for 10 minutes, dry at 80°C, grind, weigh 0.5000g into a test tube, add 10mL of pure water, keep it in a drying oven at 80°C for 12 hours, take it out, dilute to 10mL with pure water, filter with filter paper , the solution was filtered with a 0.45 μM filter membrane, and the water-soluble amino acid content was determined by the AccQ.Tag method;

[0026] (b) Chromatographic conditions: use American Waters 2695 separation unit + 2996 diode matrix detector; Milli-Q ultrapure water device (Milli-Q Company in the United States); KQ-50B ultrasonic cleaner; vacuum drying oven; 17 kinds of amino acid standard samples (provided by Waters Company); acetonitrile (chromatographically pure, Tianjin Fuchen), methanol (chromatographically pure, Tianjin Fuchen), sodium acetate-triethylamine buffer s...

Embodiment 2

[0029] Its production steps are:

[0030] (a) Preparation of sample: take konjac leaves and fix them at 115°C for 10 minutes, dry them at 80°C, grind them, weigh 0.5000g into a test tube, add 10mL of pure water, keep them overnight in a drying oven at 80°C, take them out, and use Dilute the purified water to 10 mL, filter the solution with a 0.45 μm filter membrane, and use the AQC method introduced by Waters in 1994, that is, accurately draw 10 μl of the test sample into the derivation tube, add 70 μl of buffer solution, and then vortex Add 10 μl derivative agent while stirring on the stirrer, mix well, keep it in a 60°C oven for 10 minutes, take it out, transfer it to a sample injection vial, and wait for the test;

[0031] (b) Chromatographic conditions: the instrument is American Waters 2695 separation unit + 2996 diode matrix detector, mobile phase A is 140mM sodium acetate-triethylamine buffer solution (pH4.95), mobile phase B: 100% acetonitrile, mobile phase C : 100% u...

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Abstract

The invention relates to a method for building a konjak finger print; the method adopting a high efficiency liquid phase chromatography includes the following steps: (a) the preparation of a sample: konjak tissues are taken for deactivation of enzymes, drying and trituration and then weighed in a test tube, and the constant volume and filter of the konjak tissues are made by purified water, and the sample undergoes the pre column derivation according to the analysis method for plant amide acids; (b) chromatogram conditions: the high efficiency liquid phase chromatograph or the amino acid analyzer is used, and the chromatographic column uses the octadecylsilane chemically bonded silica as fillers; (c) the detection: the sampling solution is precisely sucked and injected in the high efficiency liquid phase chromatograph, and the finger print is detected and obtained according to the high efficiency liquid phase chromatography. By analyzing the relation between characteristic peaks in the finger print and the breed, the quality, the resistance, the output and the growth state, etc. of the konjak, the invention can provide technical directions for scientific efforts such as the collection and classification of the konjak resource, the breeding selection of new breeds and the research of novel amino acid pesticides for controlling konjak diseases, etc.

Description

technical field [0001] The invention relates to a method for establishing konjac fingerprint. technical background [0002] The research, development and application of fingerprints began with the monitoring of the quality of Chinese herbal medicines. Traditional Chinese medicine fingerprints are based on the concept of forensic "fingerprints", including traditional Chinese medicine DNA fingerprints, protein fingerprints and chemical fingerprints. By fingerprints we mean chemical fingerprints. [0003] Fingerprints have two characteristics: one is that the authenticity or origin of samples can be effectively identified through the characteristics of fingerprints; the other is that the quality of products can be effectively controlled through the formulation of the area or proportion of the main characteristic peaks of fingerprints to ensure product quality. Relatively consistent quality. At present, fingerprints have become the most effective means to control the quality ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 陈永波滕建勋降巧龙牟方贵杨朝柱
Owner ACAD OF AGRI SCI ENSHI TUJIA MIAOAUTONOMOUS PREFECTURE
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