Method for authenticity identification of Maca powder and determination of Maca content
A technology of maca powder and pseudo-maca powder, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple sample pretreatment
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[0028] 1) Making samples of adulterated maca. Mix the dry pure yellow maca powder and turmeric powder in different proportions to make 16 kinds of maca powder samples, wherein the contents of maca powder are 95%, 90%, 85%, 80%, and 75% by mass respectively , 70%, 65%, 60%, 55%, 50%, 40%, 30%, 20%, 10%, 3 mixed samples for each mass percentage, 1 mixed blind sample, a total of 43 mixed samples .
[0029] 2) Make test samples. The sample to be tested was placed in a 50mL volumetric flask, distilled water was adjusted to the scale line, and the free amino acid was extracted with a 500W ultrasonic instrument for 10min, and 10mL of the extract was placed in a centrifuge tube, centrifuged at 12000rpm in a high-speed centrifuge for 5min, and passed through a 0.45μm filter membrane.
[0030] 3) An amino acid analyzer was used to measure the free amino acid spectral data of pure maca, mixed maca and manjing, and an ion exchange chromatography-ninhydrin post-column derivatization meth...
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