58 results about "Amentoflavone" patented technology

The present invention relates to compositions and methods for modulating hair growth or regrowth. The compositions of the present invention comprise extracts of one or more of the following: Boswellia serrata, Undaria pinnatifida, green tea (e.g., Camellia sinensis), shiso, Pureraria mirifica, luteolin (e.g. Perilla ocymoides leaf extract), astilbin, vitamin E, amentoflavone, tetrahydropiperine, licochalcone, astaxanthin, red clover, Brassica juncea, unfermented green rooibos, enzyme CoQ10, salvia, ximenynic acid, hops oleoresin, apple, soy, saw palmetto, or ellagic extract, or any derivative thereof. In particular, the compositions and methods of the present invention can be used to stimulate or increase hair growth and / or prevent or slow the loss of hair by having one or more of the following functions: (a) inhibiting synthesis of DHT; (b) inhibiting proteasomal activity; (c) inhibiting IL-1 activity; (d) increasing vascularization; (e) increasing expression of vascular endothelial growth factor; (f) increasing expression of keratinocyte growth factor; (g) inhibiting inflammation; or (h) acting as an antibacterial.

The invention discloses new application of a natural product amentoflavone in treating cancers. The natural product amentoflavone is extracted from plants, Chinese medicinal materials or Chinese medicaments prepared in ready-to-use forms containing the amentoflavone. The compound serves as a pharmacodynamic component to prepare medicament preparations for treating the cancers, and can be independently or combined with an excipient acceptable in the pharmaceutical technology to be prepared into the medicament preparations such as tablets, capsules, granules, sustained release preparations and pills for oral administration, gels and suppositories administrating through orifices such as vagina, anus and nasal cavity, and intramuscular or intravenous injections. The content of the amentoflavone in the medicament preparations is over 90 percent. The invention discloses the pharmacodynamic component of a Chinese medicament tamariskoid spikemoss herb for treating the cancers, and provides a scientific basis for clinical application, so the amentoflavone can serve as a natural anticancer agent and has the characteristics of no addiction and low dosage.

S. pvlvinata (Hook.et Gev.) Maxim of Selaginella of Selaginellaceae, S. tamariscina (beav.) spring, S. moellendorfii Hieron and so on are taken as raw materials for extraction and separation of total flavonoids of the Selaginella and the single component amentoflavone thereof, thus preparing a pharmaceutical preparation for prevention and treatment for Alzheimer disease.

The invention provides a method for detecting amentoflavone in Herba Selaginellae extract. The content of amentoflavone is rapidly and accurately detected through a reversed-phase high-performance liquid chromatography gradient elution method. The method has good precision, reappearance and reliability, is advanced and simple, and can safely, effectively and comprehensively control the quality of the Herba Selaginellae extract.

The invention discloses a novel use of natural amentoflavone compound in curing viral diseases. The natural amentoflavone compound is extracted from plants, Chinese medicinal materials or prepared herbal medicine in small pieces ready for decoction, which contain the components of amentoflavone. Through conventional methods, the compound is used as medicinal ingredients to prepare pharmaceutical preparations for curing viral diseases, and can be used separately or be combined with acceptable excipient in the pharmaceutical process to prepare oral preparations of troches, capsules, granules, sustained-release preparations, dropping pills and the like, to prepare gels, suppository and the like to be used in cavities such as the vagina, the anus, the nasal cavity and the like, and to prepareinjections for use in muscles or veins. The content of amentoflavone is above 90 percent. The invention discloses the effective ingredient of the Chinese herbal Selaginella in the curing viral diseases, provides a scientific basis for clinical application of Selaginella and enables amentoflavone to be used as a natural antiviral agent, and the antiviral agent causes no addiction and is small in dosage.

The invention discloses an extraction method for amentoflavone in Selaginella tamariscina. The method comprises the following steps: step 1, crushing Selaginella tamariscina and then sieving the crushed Selaginella tamariscina; step 2, weighing 0.2 part by weight of Selaginella tamariscina powder, uniformly mixing the Selaginella tamariscina powder with 1 part by weight of diatomite and standing the obtained mixture for subsequent usage; and step 3, adding a proper amount of the mixture obtained in the step 2 into an extraction pool equipped with a filtering membrane in advance until the diatomite reaches the mouth of the extraction pool, covering a cover of the extraction pool and carrying out static extraction, wherein extract is obtained after completion of extraction, and extraction conditions are that methanol is used as an extraction solvent, extraction temperature is 110 DEG C, static extraction time is 5 min, a flushing volume is 80%, and three static cycles are carried out. The extraction method for amentoflavone in Selaginella tamariscina is simple to operate and short in consumed time and lowers requirements on operation skills of the skilled.

A method for extracting cleupin and amentoflavone from olive leaf comprises the following steps of: drying olive leaf, extracting the dried olive leaf by the use of an ethanol solution, filtering, carrying out vacuum concentration, extracting, absorbing by the use of a resin, eluting with ethanol, condensing the eluate, followed by crystallization and recrystallization to prepare pure cleupin and amentoflavone. Reagents used in the invention are all nontoxic cheap chemical reagents produced in quantity. During the whole process, a mature reagent recovery routine technology can be utilized, thus greatly reducing wastes discharged into the environment. As cleupin and amentoflavone can be simultaneously extracted, production cost is greatly reduced, and the olive leaf resource is also saved.

The present invention relates to compositions and methods for modulating hair growth or regrowth. The compositions of the present invention include extracts of one or more of the following: Boswellia serrata, Undaria pinnatifida, green tea (e.g., Camellia sinensis), shiso, Pureraria mirifica, luteolin (e.g. Perilla ocymoides leaf extract), astilbin, vitamin E, amentoflavone, tetrahydropiperine, lichochalcone, astaxanthin, red clover, Brassica juncea, unfermented green rooibos, enzyme CoQ10, salvia, ximenynic acid, hops oleoresin, apple, soy, saw palmetto, or ellagic extract, or any derivative thereof.

The invention relates to an anti-cancer drug composition containing amentoflavone. The drug composition adopts the amentoflavone, swertiamarin, syringopicroside and false Chinese swertia herb as bulk drugs which are matched to prepare various dosage forms according to the conventional preparation technology. The drug composition provided by the invention has significance on preparing anti-cancer drug, especially anti-hepatoma drug, anti-lung cancer drug and anti-gastric caner drug.

The invention discloses biflavone-zinc complexes as well as a preparation method and an application thereof. Amentoflavone is taken as a ligand, zinc ions are taken as central ions, amentoflavone-zinc complexes are obtained through a reaction, and structures of the complexes are characterized with infrared spectra, ultraviolet-visible absorption spectra and high-resolution mass spectra. Meanwhile, the anti-tumor activity and the anti-oxidation activity of the amentoflavone-zinc complexes are studied. An MTT method proves that the amentoflavone-zinc complexes have better anti-tumor activity which is higher than that of amentoflavone; a pyrogallol auto-oxidation method and an ABTS method prove that the anti-oxidation activity of the amentoflavone-zinc complexes is higher than that of amentoflavone. By means of forming of the amentoflavone-zinc complexes, the anti-tumor activity and the anti-oxidation activity of amentoflavone are improved, and the complexes are expected to be used for developing drugs.

The invention discloses a biflavone-manganese complex, as well as a preparation method and an application of the biflavone-manganese complex. The amentoflavone-manganese complex is prepared by taking amentoflavone as a ligand and a manganese ion as a central ion for reaction, and a structure of the complex is characterized by an infrared spectrum, an ultraviolet-visible absorption spectrum and a high resolution mass spectrum. At the same time, the antitumor activity and the oxidation resistance activity of the amentoflavone-manganese complex are researched. An MTT (methyl thiazolyl tetrazolium) method shows that the amentoflavone-manganese complex has the better antitumor activity than amentoflavone; and both a pyrogallol autoxidation method and an ABTS (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) method show that the amentoflavone-manganese complex has the better oxidation resistance activity than amentoflavone. Due to the formation of the biflavone-manganese complex, the antitumor activity and the oxidation resistance activity of amentoflavone are improved, and the biflavone-manganese complex is expected to be used for drug development.

The invention discloses a purifying method for amentoflavone. The method comprises the following steps: 1) acquiring a selaginella total flavone extract, wherein the content of amentoflavone in the selaginella total flavone extract is not less than 30%; 2) dissolving the selaginella total flavone extract by methanol or ethanol, feeding in an ADS-7 macroporous resin column, carrying out water washing of the resin column until the effluent is clarified, eluting with a 0.5-2 v / v% hydrochloric acid, and collecting an eluate; and 3) adjusting the pH of the eluate to 4-6, concentrating, standing for crystallization, collecting crystals, carrying out recrystallization of the crystals with methanol or ethanol, filtering, collecting crystals, drying, and thus obtaining the product. ADS-7 macroporous resin not only having an adsorption function but also having an ionic exchange function is adopted for chromatography of the selaginella total flavone extract, while amentoflavone is adsorbed on the macroporous resin, a quite good impurity removal effect is played, the product with the purity of up to 95% or more is obtained, and the product yield is 71% or more.

The present invention relates to method of identifying Rock lily plant and its preparation. The method includes alcohol extraction of Jiangnan Rock lily and positive contrast with amentoflavone. The present invention has the identification of Jiangnan Rock lily medicine material and preparation from other 10 kinds of confused Rock lily, including Yanzhou Rock lily, dark green Rock lily, etc. The quality control method has high specialization and high stability, and may be used in controlling the quality of Jiangnan Rock lily medicine material and its products, such as cosmetics, health product and medicine.

The invention belongs to the field of analytical chemistry, and relates to a method for extracting an effective component from a traditional Chinese medicine, in particular to a method for infrared extraction of amentoflavone from herba selaginellae. The method provided by the invention adopts a solution of a herba selaginellae sample irradiated by an infrared lamp; after a certain period of extraction, the extract is treated, and a high performance liquid chromatograph is used for carrying out separation and analysis. Qualitative and quantitative analysis is performed based on chromatographicinformation of the sample and a reference substance. The method is fast, environment-friendly and high in recovery rate.

The invention discloses a method for analyzing amentoflavone in selaginella tamariscina. The method is characterized by comprising the following steps: 1, pulverizing the selaginella tamariscina, and then obtaining extract liquor through an ASE extraction method, wherein equipment adopted according to the ASE extraction method is an ASE rapid extraction instrument, the extraction condition is that extracting solvent is methyl alcohol, the extraction temperature is 110 DEG C, static extraction time is 5 minutes, the flushing volume is 80%, and the number of static circulation times is 3; 2, conducting dilution and centrifugation on the extract liquor obtained in step 1, so that supernatant is obtained; 3, testing the amentoflavone content in the supernatant obtained in step 2 through a liquid chromatography. The method for analyzing the amentoflavone in the selaginella tamariscina is high in detection speed and high in detection accuracy.

The invention relates to the field of food detection, in particular to a method for evaluating authenticity of lotus bee pollen. According to the invention, firstly, the application of high-content amentoflavone (AF) as a lotus bee pollen characteristic marker is discovered. Accordingly, the invention provides the method for evaluating the authenticity of the lotus bee pollen, the AF is used as anindicative substance, and when the content of the AF in the lotus bee pollen is 600-850 mg / kg, the AF can be judged as real lotus bee pollen; otherwise, the bee pollen is judged as other types of beepollen or adulterated lotus bee pollen. The method provided by the invention also has the advantages of simplicity, accuracy and the like, is convenient to operate and popularize, and has important practical significance for protecting the legitimate rights and interests of lotus bee pollen consumers and maintaining the healthy development of the bee product consumption industry.

The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a method for separating and purifying amentoflavone, in particular, a method for enriching and purifying the amentoflavone through a high speed countercurrent chromatography method. The method is characterized in that: two mutually-immiscible solvent systems are utilized to perform the separation and purification in a mode of high-speed planetary motion in a support pipe; the method is applicable for the countercurrent chromatographies with various types to separate the amentoflavone, and has advantages of no irreversible adsorption, no loss of sample, no pollution, high efficiency and speed, high recovery rate, mild separating environment, high product purity and large quantity separation, and is applicable for industrial production.

The invention relates to a method for supercritical fluid extraction of amentoflavone. In the method, a supercritical CO2 extraction-column chromatography-recrystallization combination technology is adopted to prepare high-purity amentoflavone; the conditions of the supercritical CO2 extraction are as follows: ethanol or acetone is used as an entrainer, the flow of CO2 is 22-35L / h, the pressure of an extraction kettle is 23-38MPa, the temperature of the extraction kettle is 35-50 DEG C, and extraction time is 3-6 hours. The method specifically comprises the following steps: carrying out supercritical extraction with the parameters; analyzing so as to obtain extractive; separating and purifying the extractive through polyamide column chromatography; eluting with chloroform-acetone-methanol; and recrystallizing with an ethanol aqueous solution so as to obtain the finished product. The separation method is simple and is easy to apply in industrial production, and the product is pure.

The invention discloses a method for extracting and separating high-purity amentoflavone from a selaginella plant. The method comprises the following steps: separating an amentoflavone crude product from a selaginella plant alcohol extract by taking non-polar macro-porous resin as a separation material with a hydrous ethanol-pH gradient elution process; and separating to obtain the high-purity amentoflavone through the processes of alkaline methanol dissolving acidic precipitation and ethanol recrystallization. The amentoflavone is 98 percent or above in content, and is 50 percent or above inprocess transfer rate.

The invention relates to a construction method of an HPLC fingerprint of Qian Bai Bi Yan Pian. The method comprises the steps of: carrying out preparation of a mixed reference substance solution, wherein hyperoside, p-hydroxycinnamic acid, notopterol, chrysophanol, isoimperatorin, chlorogenic acid, imperatorin, amentoflavone, physcion, emodin, osthole, caffeic acid and kaempferol reference substances are added into a solvent to obtain the mixed reference substance solution; carrying out preparation of a test substance solution, wherein a sample of Qian Bai Bi Yan Pian is added into methanol with a volume fraction of 25%-100% for extraction, and subsequent filtrate is obtained after filtration of extract to obtain the test substance solution; and respectively injecting the mixed reference substance solution and the test substance solution into a high performance liquid chromatography, and carrying out measurement and determination to obtain the fingerprint. The constructed fingerprint can comprehensively reflect quality information of Qian Bai Bi Yan Pian, has the advantages of fastness, stability, high precision, high reproducibility and the like, and can be used for quality control of Qian Bai Bi Yan Pian.

The invention relates to a pharmaceutical composition for treating AIDS. The pharmaceutical composition is prepared from raw materials of amentoflavone, cucurbitacin, peimine and protostemonine in certain proportion; and the pharmaceutical composition can be prepared into various dosage forms according to the conventional preparation process. The pharmaceutical composition provided by the invention has great significance to the preparation of medicaments for the treatment of AIDS.

The invention discloses application of amentoflavone in preparation of a medicine for promoting wound healing. The amentoflavone is a flavonoid small molecule compound, and a brand new agonist is developed on the basis of a novel target point GPR39 for promoting wound healing. Experiments prove that the amentoflavone has obvious activity of promoting wound healing by exciting a GPR39 target spot, and can be used for research and development of medicines for promoting wound healing.

The invention relates to water treatment chemical for removing para-nitroaniline in water. The water treatment chemical is made from 3, 7-dimethyl-6-octenal, 4-formylphenylBeta-D-allopyranoside, aluminum sulfate, trans, trans-2, 4-nonadienal, 2-hydroxyl-4-isopropyl-2, 4, 6-cycloheptatriene-1-one, apha-aminoisocaproic acid, potassium alginate, picrasinol B, (S)-2-(fmoc-amino)caproic acid, acutumidine, 1-pentadecanol, 3, 7, 11-trimethyl-1, 6, 10-dodecatriene-3-ol, amentoflavone, and 2-ethyl-2, 5-dimethylhexanoic acid. The water treatment chemical has the advantages that the chemical has high ability to complex with the para-nitroaniline, complex precipitant forms fast, the removal rate of the para-nitroaniline is up to 99%, and the chemical is low in toxicity, low in use amount, free of harm to water and low in treatment cost.

The invention relates to a pharmaceutical composition for treating AIDS. The pharmaceutical composition is prepared from raw materials of amentoflavone, cucurbitacin, peimine and gomisin G in certain proportion; and the pharmaceutical composition can be prepared into various dosage forms according to the conventional preparation process. The pharmaceutical composition provided by the invention overcomes the defects of the prior art, and has great significance to the preparation of medicaments for the treatment of AIDS.

The invention relates to the medical use of amentoflavone in treatment of chicken necrotic enteritis. The clostridium perfringen sliding motion inhibition test, the biofilm formation inhibition test,the chicken necrotic enteritis model treatment test, and the histopathological section observation and intestinal flora analysis test confirm that the amentoflavone has protective effects against chicken necrotic enteritis caused by clostridium perfringen infection. Due to the abuse of traditional antibiotics and the continuous enhancement of bacterial drug resistance, the amentoflavone has the characteristics of high cure rate, no drug resistance and drug residues. Therefore, the amentoflavone is used for treating chicken necrotic enteritis, which can increase the selectivity of use of drugs,and has an important significance for the development of new drugs.

The invention discloses a method for detecting the content of various biflavones in a ginkgo leaf preparation. The method can detect amentoflavone, ginkgetin, isoginkgetin, bilobetin and sciadopitysinin the ginkgo leaf preparation simultaneously, an HPLC(Waters e2695) and Welch Bolimate C18 column is adopted for determination; a mobile phase is acetonitrile-water, and gradient elution is conducted, wherein the volume fraction of acetonitrile is 40%-80%, the flow velocity is 0.5 ml / min, the detection wavelength is 330 nm, and the column temperature is 30 DEG C; automatic sample introduction isconducted on standard substances and samples for test separately, and the content of five biflavones is calculated with an external standard method. Accordingly, by means of detection of the biflavone content, the quality control standard of the ginkgo leaf preparation is greatly improved; complex pretreatment is not needed, rapid detection can be achieved, the detection time is saved, and the detection cost is reduced.

The invention discloses a special settling agent for tail gas of a rural-domestic-garbage incinerator. The special settling agent is prepared by the following components in parts by weight: 100 parts of tertiary butanol, 10 parts of magnetized water, 2 parts of chitosan, 1 part of saponin, 0.5 part of watermelon polysaccharide, 3 parts of low-sodium salt, 0.5 part of wheat oligopeptides, 0.5 part of lutein ester, 0.04 part of amentoflavone, 0.2 part of baicalin, 1.5 parts of softening agent, 2 parts of water-based resin, 2.5 parts of cationic starch, 2 parts of urea, 0.06 part of sodium sulfide and 0.06 part of rare-soil oxide. The special settling agent disclosed by the invention has the advantages that the traditional pure water filtering is upgraded, the gas-filtering settling agent is innovated by experiments, and the tail gas generated by garbage incineration is treated, so that the exhausted tail gas does not contain any pollutant, and the special settling agent is environmental-friendly to atmosphere and has no harm to a human body.

The invention discloses a purifying method for amentoflavone. The method comprises the following steps: 1) acquiring a selaginella total flavone extract, wherein the content of amentoflavone in the selaginella total flavone extract is not less than 30%; 2) dissolving the selaginella total flavone extract by methanol or ethanol, feeding in an ADS-7 macroporous resin column, carrying out water washing of the resin column until the effluent is clarified, eluting with a 0.5-2 v / v% hydrochloric acid, and collecting an eluate; and 3) adjusting the pH of the eluate to 4-6, concentrating, standing for crystallization, collecting crystals, carrying out recrystallization of the crystals with methanol or ethanol, filtering, collecting crystals, drying, and thus obtaining the product. ADS-7 macroporous resin not only having an adsorption function but also having an ionic exchange function is adopted for chromatography of the selaginella total flavone extract, while amentoflavone is adsorbed on the macroporous resin, a quite good impurity removal effect is played, the product with the purity of up to 95% or more is obtained, and the product yield is 71% or more.

The invention relates to application of amentoflavone in research and development of medicines for treating pneumonia. Rabbit erythrocyte haemolysis tests, human pulmonary epithelial cell (A549) injury protection tests and a mouse streptococcus pneumonia model prove that the amentoflavone has a treatment effect on infection of streptococcus pneumonia. Compared with treatment with antibiotics, the amentoflavone for treatment has the characteristics of no drug resistance and high protection performance.

The invention discloses a method for determining amentoflavone content in selaginella tamariscina by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography), and belongs to the field of chemical detection. The method has the advantages of short extraction time and high detection precision. In the method, selaginella tamariscina powder is extracted by an ASE method, a methanol extract is collected; and the amentoflavone content in the methanol extract is determined by a HPLC method. The method can replace a method in the pharmacopoeia to extract amentoflavone in the selaginella tamariscina and determine the amentoflavone content in the selaginella tamariscina.