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Purifying method for amentoflavone

A kind of technology of abiflavonoid and purification method, which is applied in the field of purification of abiflavonoids of Suicha, can solve the problems of large initial investment, difficult process control, high energy consumption, etc., and achieve a good effect of impurity removal

Inactive Publication Date: 2014-04-02
GUILIN SANBAO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of this method is relatively simple, and can obtain the biflavonoid product of A. spicanus with higher content (more than 65%), but due to the use of high molecular weight polyethylene glycol, the process is difficult to control; in addition, in the extraction step, the extraction The oil in the liquid will dissolve in the extraction reagent, resulting in more impurities in the product, and it is not easy to dry; moreover, the cost of the ultrafiltration and nanofiltration equipment used in this method is high, the energy consumption is also high during operation, and the initial investment is large.

Method used

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  • Purifying method for amentoflavone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Crush the raw material of Selaginella to 20-40 mesh, take 100kg, add water 8 times the weight of the raw material, heat to 80°C for extraction for 1.5h, filter, discard the filtrate, and leave the medicinal material of Selaginella;

[0024] 2) After water extraction, add 80% (v / v) ethanol of 10 times the weight of raw materials to Selaginella medicinal materials, heat and reflux for extraction for 1.5 hours, filter, and collect the filtrate; add 80% (v / v) of 7 times the weight of raw materials to the filter residue Ethanol, heated and refluxed for 1.5h, filtered, and the filtrate was collected; the filtrate was combined to obtain an extract, the extract was concentrated to recover ethanol, and then concentrated to an extract that was 1.5 times the weight of the raw material;

[0025] 3) The extract was cooled and left standing for 12 hours, centrifuged, and the filter cake was collected;

[0026] 4) crush the filter cake, add No. 6 solvent oil of 7 times the weight o...

Embodiment 2

[0029] 1) Crush the raw material of Selaginella to 20 mesh, take 200kg, add water 5 times the weight of the raw material and heat to 100°C to extract for 0.5h, filter, discard the filtrate, and leave the medicinal material of Selaginella;

[0030]2) Add 95% (v / v) ethanol 10 times the weight of the raw material to the Selaginella medicinal material after water extraction, heat and reflux for extraction for 1 hour, filter, and collect the filtrate; add 95% (v / v) ethanol 6 times the weight of the raw material to the filter residue , heating to reflux for extraction for 1 h, filtering, and collecting the filtrate; combining the filtrates to obtain an extract, concentrating the extract to recover ethanol, and then concentrating to an extract that is twice the weight of the raw material;

[0031] 3) The extract was cooled and left standing for 4 hours, centrifuged, and the filter cake was collected;

[0032] 4) Crush the filter cake, add petroleum ether degreasing in an amount 5 tim...

Embodiment 3

[0035] 1) Crush the raw material of Selaginella to 10-20 mesh, take 150kg, add water 10 times the weight of the raw material, heat to 60°C for extraction for 2 hours, filter, discard the filtrate, and leave the medicinal material of Selaginella;

[0036] 2) After water extraction, add 70% (v / v) ethanol 12 times the weight of the raw material to the Selaginella medicinal material, heat and reflux for extraction for 2 hours, filter, and collect the filtrate; add 75% (v / v) ethanol 9 times the weight of the raw material to the filter residue , heating to reflux for extraction for 2 hours, filtering, and collecting the filtrate; combining the filtrates to obtain an extract, concentrating the extract to recover ethanol, and then concentrating to an extract of 1 times the amount of raw materials;

[0037] 3) The extract was cooled and left standing for 4 hours, centrifuged, and the filter cake was collected;

[0038] 4) Crush the filter cake, add No. 6 solvent oil 10 times the weight...

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Abstract

The invention discloses a purifying method for amentoflavone. The method comprises the following steps: 1) acquiring a selaginella total flavone extract, wherein the content of amentoflavone in the selaginella total flavone extract is not less than 30%; 2) dissolving the selaginella total flavone extract by methanol or ethanol, feeding in an ADS-7 macroporous resin column, carrying out water washing of the resin column until the effluent is clarified, eluting with a 0.5-2 v / v% hydrochloric acid, and collecting an eluate; and 3) adjusting the pH of the eluate to 4-6, concentrating, standing for crystallization, collecting crystals, carrying out recrystallization of the crystals with methanol or ethanol, filtering, collecting crystals, drying, and thus obtaining the product. ADS-7 macroporous resin not only having an adsorption function but also having an ionic exchange function is adopted for chromatography of the selaginella total flavone extract, while amentoflavone is adsorbed on the macroporous resin, a quite good impurity removal effect is played, the product with the purity of up to 95% or more is obtained, and the product yield is 71% or more.

Description

technical field [0001] The invention relates to a method for extracting active components in plants, in particular to a method for purifying the amiflavone from A. spicosa. Background technique [0002] The 2005 edition of the Chinese Pharmacopoeia stipulates that Selaginella is the dried whole herb of Selaginella tamariscina (Beauv.) Spring or Selaginella pulvinata (Hook. et Grev.) Maxim. Selaginella is not only ornamental, but also medicinal, and the whole plant has the effects of stopping bleeding and astringing. Folks burn the whole plant to ashes, take it orally to treat various hemorrhages, and mix it with vegetable oil and use it externally to treat various cuts. Modern medicine has proved that it has the functions of clearing heat and detoxifying, promoting blood circulation and removing blood stasis, hemostasis and anti-cancer, anti-cancer, anti-cancer, anti-infection, anti-allergic, analgesic, hypnotic, lowering blood pressure, lowering blood sugar, enhancing huma...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 卢照凯钟德品谢冬养唐利萍卓振松周晓青卢桦香应启实
Owner GUILIN SANBAO PHARMA
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