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Method for extracting cleupin and amentoflavone from olive leaf

A kind of technology of biflavone and oleuropein of Suicha spica, applied in the field of natural product chemistry, can solve the problems of long separation process, reduced content of active ingredients, low yield of active ingredients, etc., and achieve the effect of reducing production costs and saving resources

Active Publication Date: 2012-10-17
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The separation process of this method is too long, resulting in a low yield of active ingredients. In addition, the desorption solution is directly spray-dried, and the metal ions in the desorption solution exist in the product in the form of salt, and other impurities will also reduce the content of its active ingredients.

Method used

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  • Method for extracting cleupin and amentoflavone from olive leaf
  • Method for extracting cleupin and amentoflavone from olive leaf
  • Method for extracting cleupin and amentoflavone from olive leaf

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Weigh 100kg of olive leaves, pulverize them with an ordinary pulverizer, add 400L of 60% ethanol to mix, heat to about 50°C for reflux extraction for 10 hours, filter twice with three layers of gauze and once with a ceramic membrane; After the residue, concentrate under reduced pressure at 50°C until there is no ethanol smell, and obtain 80L of concentrated solution; extract the concentrated solution with 200L of ethyl acetate three times, separate and obtain three ethyl acetate layers, combine 220L of ethyl acetate extract, Concentrate the extract under reduced pressure at 50°C until 2.65kg of dry matter is obtained; dissolve the dry matter with 2.65kg of water, adsorb on H-103 macroporous resin, elute with 40% ethanol, and collect the eluate 40L of Ⅰ, then eluted with 70% ethanol, collected 30L of eluate II; concentrated the eluent Ⅰ to 8L under reduced pressure, added 24L of absolute ethanol at 4°C for crystallization and recrystallization, and obtained oleuropein 1.1...

Embodiment 2

[0030] Weigh 200kg of olive leaves, pulverize them with an ordinary pulverizer, add 600L of 60% ethanol to mix, heat to about 50°C for reflux extraction for 10 hours, filter twice with three layers of gauze and once with a ceramic membrane; After the residue, concentrate under reduced pressure at 50°C until there is no ethanol smell, and obtain 150L of concentrated solution; extract the concentrated solution with 350L of ethyl acetate three times, separate and obtain three ethyl acetate layers, and combine 380L of ethyl acetate extracts, Concentrate the extract under reduced pressure at 50°C until 5.48kg of dry matter is obtained; dissolve the dry matter with 5.48kg of water, adsorb on H-103 macroporous resin, elute with 40% ethanol, and collect the eluate Ⅰ60L, and then eluted with 70% ethanol, collected 45L of eluate II; concentrated the eluent Ⅰ to 12L under reduced pressure, added 36L of absolute ethanol to crystallize and recrystallize at 4°C, and obtained 2.35kg of oleuro...

Embodiment 3

[0032] Weigh 500kg of olive leaves, pulverize them with an ordinary pulverizer, add 2000L of 60% ethanol to mix, heat to about 50°C for reflux extraction for 10 hours, filter twice with three layers of gauze and once with a ceramic membrane; After the residue, concentrate under reduced pressure at 50°C until there is no ethanol smell, and obtain 300L of concentrated solution; extract the concentrated solution with 800L of ethyl acetate three times, separate and obtain three ethyl acetate layers, and combine 850L of ethyl acetate extracts, Concentrate the extract under reduced pressure at 50°C until 11.28 kg of dry matter is obtained; dissolve the dry matter with 11.28 kg of water, adsorb on H-103 macroporous resin, elute with 40% ethanol, and collect the eluate 150L of Ⅰ, then eluted with 70% ethanol, collected 120L of eluate II; concentrated the eluate Ⅰ to 30L under reduced pressure, added 90L of absolute ethanol at 4°C for crystallization and recrystallization, and obtained ...

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Abstract

A method for extracting cleupin and amentoflavone from olive leaf comprises the following steps of: drying olive leaf, extracting the dried olive leaf by the use of an ethanol solution, filtering, carrying out vacuum concentration, extracting, absorbing by the use of a resin, eluting with ethanol, condensing the eluate, followed by crystallization and recrystallization to prepare pure cleupin and amentoflavone. Reagents used in the invention are all nontoxic cheap chemical reagents produced in quantity. During the whole process, a mature reagent recovery routine technology can be utilized, thus greatly reducing wastes discharged into the environment. As cleupin and amentoflavone can be simultaneously extracted, production cost is greatly reduced, and the olive leaf resource is also saved.

Description

technical field [0001] The invention belongs to the technical field of natural product chemistry, and in particular relates to a method for separating and extracting high-purity oleuropein and diflavonoids from olive leaves. Background technique [0002] Olive (Olea europaea L.), also known as foreign olive and olean fruit, is a plant of the genus Olea L in the family Oledceae. Olives are native to Asia Minor and have been introduced into my country. The existing olive area is about 500,000 mu. [0003] Olive leaves and fruits are used as medicine to treat various diseases. The decoction or infusion of olive leaves is often used as folk medicine for diabetes in Mediterranean countries, which has certain curative effect. Olive leaf extract can be used as a dietary supplement to enhance immune function. Existing data show that olive has anti-arrhythmia, anti-fungal, anti-high cholesterol, lowering blood sugar, lowering blood pressure, anti-inflammation, anti-oxidation, anti-...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08C07D311/30C07D311/40
Inventor 李兰岚王斯成代洪波
Owner HUNAN AGRICULTURAL UNIV
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