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Method for infrared extraction of amentoflavone from herba selaginellae

A technology of amiflavonoids and selaginella, which is applied in the field of analytical chemistry, can solve the problems of infrared extraction of amiflavones from selaginella, and achieve the effects of industrial application, environmental protection and low cost

Inactive Publication Date: 2019-02-12
FUDAN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

But so far, there is no method for infrared extraction of biflavonoids from Selaginella chinensis

Method used

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  • Method for infrared extraction of amentoflavone from herba selaginellae
  • Method for infrared extraction of amentoflavone from herba selaginellae

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Embodiment

[0038] 1. Instruments and reagents

[0039] 1) Analytical instrument

[0040] The infrared lamp was purchased from Haining Andison Lighting Co., Ltd., the HH-2 digital display constant temperature water bath was purchased from Jintan Jerry Electric Co., Ltd., the GB204 electronic balance was purchased from METTLER TOLEDO, and the EX125DZH electronic balance was purchased from Ohhao Sri Lankan Instrument Co., Ltd., and TDL80-2B centrifuge was purchased from Shanghai Anting Scientific Instrument Factory.

[0041] Shimadzu LC-15C high-performance liquid chromatograph was purchased from Japan Shimadzu Corporation, the analysis software was LCsolution 15C data processing system, and the chromatographic column was Xtimate TM C18 (4.6*250mm, 5μm), purchased from Shanghai Welch Company.

[0042] 2) Test drugs

[0043]The standard product of amiflavones from Suicha chinensis (CAS: 1617-53-4, content >98%) was purchased from Chengdu Zhibiao Pure Biotechnology Co., Ltd. Methanol (ch...

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Abstract

The invention belongs to the field of analytical chemistry, and relates to a method for extracting an effective component from a traditional Chinese medicine, in particular to a method for infrared extraction of amentoflavone from herba selaginellae. The method provided by the invention adopts a solution of a herba selaginellae sample irradiated by an infrared lamp; after a certain period of extraction, the extract is treated, and a high performance liquid chromatograph is used for carrying out separation and analysis. Qualitative and quantitative analysis is performed based on chromatographicinformation of the sample and a reference substance. The method is fast, environment-friendly and high in recovery rate.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and relates to a method for extracting active ingredients in traditional Chinese medicines, in particular to a method for infrared extraction of amiflavonoids from Selaginella chinensis. Background technique [0002] Traditional Chinese medicine extraction methods, such as dipping, soak Chinese medicine to extract the active ingredients, such as decoction, similar to our traditional Chinese medicine, etc. Practice shows that traditional Chinese medicine extraction methods are difficult to meet the current scientific needs. Therefore, some new extraction methods of traditional Chinese medicine have begun to attract the attention of those skilled in the art, such as microwave extraction technology, which uses microwaves to make the active ingredients in the drug enter the solution; such as enzymatic extraction technology by adding specific Enzymes, so as to improve the extraction efficiency; liq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40G01N30/02
CPCG01N30/02C07D311/30C07D311/40
Inventor 段更利郁颖佳徐琛汪洋江洁冰龙佳坤
Owner FUDAN UNIV
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