Application of amentoflavone in preparation of medicine for promoting wound healing

A technology of biflavonoids and wound healing, which is applied in the field of natural compounds of traditional Chinese medicine, can solve the problems that traditional methods are difficult to meet clinical wound treatment, limited donor skin sources, carcinogenicity, etc., and achieve the effect of promoting wound healing activity

Pending Publication Date: 2022-01-07
CHINA PHARM UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0002] Skin wounds are a common clinical symptom, and conventional skin wound treatments include skin grafting and artificial substitute coverage, however, due to the limited source of donor skin, the risk of immune rejection or infectious diseases, and the high cost of artificial skin, traditional methods Difficult to meet the needs of clinical wound treatment
Although some growth factors have shown strong effects in promoting wound healing, their safety issues have been the focus of attention due to the complex compatibility and carcinogenic potential of multiple growth factors involved in wound healing
[0004] However, the discovery of GPR39 agonists with significant wound-healing activity is still lacking.

Method used

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  • Application of amentoflavone in preparation of medicine for promoting wound healing
  • Application of amentoflavone in preparation of medicine for promoting wound healing
  • Application of amentoflavone in preparation of medicine for promoting wound healing

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Embodiment 1

[0018] The structural formula of the biflavonoids figure 1 , the molecular formula is C 30 h 18 o 10 , is a flavonoid small molecule natural product compound derived from Selaginella tamariscina (Beauv.) Spring, a plant of the Selaginaceae family. Using a high-throughput real-time fluorescence detection system Evaluation of the activity of biflavones on heterologously expressed hGPR39 receptors, the results showed that biflavones had strong agonistic activity on GPR39, EC s0 154nM, such as figure 2 .

[0019] MTT assay and BrdU incorporation assay were used to verify the effect of biflavonoids on the proliferation of keratinocytes, and the expression of GPR39 was knocked down by transfection of siRNA to investigate whether these effects depend on GPR39. The results are as follows image 3 , Amiflavone promotes the proliferation of HaCaT cells through GPR39 (A: the solvent control group is 1, and the values ​​of each group are multiples of the solvent control group), *...

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Abstract

The invention discloses application of amentoflavone in preparation of a medicine for promoting wound healing. The amentoflavone is a flavonoid small molecule compound, and a brand new agonist is developed on the basis of a novel target point GPR39 for promoting wound healing. Experiments prove that the amentoflavone has obvious activity of promoting wound healing by exciting a GPR39 target spot, and can be used for research and development of medicines for promoting wound healing.

Description

technical field [0001] The invention relates to a new application of natural compounds of traditional Chinese medicines, in particular to the effect of biflavonoids of spicantha chinensis in promoting wound healing. Background technique [0002] Skin wounds are a common clinical symptom, and conventional skin wound treatments include skin grafting and artificial substitute coverage, however, due to the limited source of donor skin, the risk of immune rejection or infectious diseases, and the high cost of artificial skin, traditional methods It is difficult to meet the needs of clinical wound treatment. Although some growth factors have shown strong effects in promoting wound healing, their safety issues have been the focus of attention due to the complex compatibility and carcinogenic potential of multiple growth factors involved in wound healing. [0003] G protein-coupled receptor GPR39 is mainly expressed in skin keratinocytes in the skin system and can be activated by i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61P17/02A61K36/11
CPCA61K31/352A61P17/02A61K36/11
Inventor 曹征宇张凡张壮陈颐宸张嫔慧
Owner CHINA PHARM UNIV
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