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Method for determining amentoflavone content in selaginella tamariscina by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)

A technology of double flavonoids and Selaginella paniculata, applied in the field of chemical detection, can solve the problems of high professional level of technicians and long reflux time, etc.

Inactive Publication Date: 2018-06-15
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The method has a longer reflow time and requires a higher level of professionalism for technicians

Method used

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  • Method for determining amentoflavone content in selaginella tamariscina by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)
  • Method for determining amentoflavone content in selaginella tamariscina by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)
  • Method for determining amentoflavone content in selaginella tamariscina by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography)

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Instruments and reagents

[0025] 1.1 Instruments:

[0026] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), ThermoU3000 UHPLC liquid chromatograph

[0027] 1.2 Reagents:

[0028] Water: Class I water in accordance with GB / T 6682;

[0029] Methanol: chromatographically pure;

[0030] Diatomaceous earth: 2mm;

[0031] 2. Method

[0032] 2.1 Preparation of reference solution

[0033] Take an appropriate amount of Biflavone Reference Substance, accurately weighed, add methanol to make a solution containing 25μg per 1ml, and get it.

[0034] 2.2 Preparation of the test solution

[0035] The 2015 edition pharmacopoeia method: take about 0.2g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol accurately, weigh it, heat and reflux for 5 hours, let it cool, and weigh again Determine the weight, use methanol to make up the lost weight, shake w...

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Abstract

The invention discloses a method for determining amentoflavone content in selaginella tamariscina by ASE(accelerated solvent extraction)-HPLC (high-performance liquid chromatography), and belongs to the field of chemical detection. The method has the advantages of short extraction time and high detection precision. In the method, selaginella tamariscina powder is extracted by an ASE method, a methanol extract is collected; and the amentoflavone content in the methanol extract is determined by a HPLC method. The method can replace a method in the pharmacopoeia to extract amentoflavone in the selaginella tamariscina and determine the amentoflavone content in the selaginella tamariscina.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to an ASE-HPLC method for measuring the content of biflavonoids in Selaginella spica. Background technique [0002] The 2015 edition pharmacopoeia method: take about 0.2g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a stoppered conical flask, add 50ml of methanol accurately, weigh it, heat and reflux for 5 hours, let it cool, and weigh again Determine the weight, use methanol to make up the lost weight, shake well, filter, and take the filtrate to obtain the final product. [0003] The reflux time of this method is longer, and the professional level of technicians is higher. Contents of the invention [0004] In view of the above deficiencies, the present invention aims to provide a method for determining the content of biflavonoids in Selaginella chinensis by ASE-HPLC method, the method has short extraction time and high detection...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 张云平黄林杰赖秀梅
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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