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Method for detecting amentoflavone in Herba Selaginellae extract

The invention relates to a technology of biflavonoids and a detection method, which are applied in the field of traditional Chinese medicine detection and can solve the problems of long analysis time, late retention time of biflavonoids, and unsatisfactory peak symmetry.

Active Publication Date: 2014-12-31
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the actual operation process, the retention time of the biflavonoids of Suicarina spiconiae is late, and the analysis time is long, which leads to unsatisfactory peak symmetry
At the same time, in the published patents, they are all about the preparation and application, research progress and pharmacological activity of the biflavones of Selaginella chinensis, and there are no reports related to the present invention or similar

Method used

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  • Method for detecting amentoflavone in Herba Selaginellae extract
  • Method for detecting amentoflavone in Herba Selaginellae extract
  • Method for detecting amentoflavone in Herba Selaginellae extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1 Chromatographic conditions and system adaptability

[0021] Chromatographic column: C18 chromatographic column (Agilent TC-C18(2) 4.6×250mm, 5μm); using methanol solution as mobile phase A and 0.1% TFA aqueous solution as mobile phase B, carry out gradient elution as specified in the table below, Flow rate: 1ml / min, column temperature 30°C, detection wavelength 335nm.

[0022]

[0023] 2 Experimental methods and results

[0024] 2.1 Preparation of reference solution

[0025] Precisely weigh 5.26 mg of the reference substance of Suicha chinensis biflavone, put it in a 10ml measuring bottle, add an appropriate amount of methanol to dissolve and dilute to volume, and use it as the reference solution.

[0026] 2.2 Preparation of the test solution

[0027] Accurately weigh 50.14mg of Selaginella extract, put it in a 50ml measuring bottle, add a certain amount of methanol to dissolve, ultrasonicate for 10 minutes, let it cool, and then dilute to the mark with methanol...

Embodiment 2

[0032] 1 Chromatographic conditions and system adaptability

[0033] Chromatographic column: C18 chromatographic column (Agilent TC-C18(2) 4.6×250mm, 5μm); using methanol solution as mobile phase A and 0.1% TFA aqueous solution as mobile phase B, carry out gradient elution as specified in the table below, Flow rate: 1.2ml / min, column temperature 35°C, detection wavelength 330nm.

[0034]

[0035] 2 Experimental methods and results

[0036] 2.1 Preparation of reference solution

[0037] Accurately weigh 10.08mg of the reference substance of Suicha chinensis biflavone, put it in a 10ml measuring bottle, add an appropriate amount of methanol to dissolve it, and then set the volume to the mark to obtain it.

[0038] 2.2 Preparation of the test solution

[0039] Accurately weigh 25.78mg of Selaginella extract sample, put it in a 50ml measuring bottle, add a certain amount of methanol to dissolve, ultrasonicate for 20 minutes, let it cool, and then dilute to the mark with meth...

Embodiment 3

[0044] 1 Chromatographic conditions and system adaptability

[0045] Chromatographic column: C18 chromatographic column (Agilent TC-C18(2) 4.6×250mm, 5μm); using methanol solution as mobile phase A and 0.1% TFA aqueous solution as mobile phase B, carry out gradient elution as specified in the table below, Flow rate: 0.8ml / min, column temperature 25°C, detection wavelength 337nm.

[0046]

[0047] 2 Experimental methods and results

[0048] 2.1 Preparation of reference solution

[0049] Accurately weigh 7.25 mg of Amiflavone of Suicha chinensis, put it in a 10ml measuring bottle, add an appropriate amount of methanol to dissolve and make it to volume, and use it as the reference substance solution.

[0050] 2.2 Preparation of the test solution

[0051] Accurately weigh 30.21mg of Selaginella extract, put it in a 50ml measuring bottle, add a certain amount of methanol to dissolve, ultrasonicate for 15 minutes, let it cool, and then dilute to the mark with methanol to obtai...

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Abstract

The invention provides a method for detecting amentoflavone in Herba Selaginellae extract. The content of amentoflavone is rapidly and accurately detected through a reversed-phase high-performance liquid chromatography gradient elution method. The method has good precision, reappearance and reliability, is advanced and simple, and can safely, effectively and comprehensively control the quality of the Herba Selaginellae extract.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicines, and relates to a detection method of amiflavonoids in Selaginella spica extract. Background technique [0002] Selaginella is the dry whole herb of Selaginella tamariscina (Beauv) Spring or Selaginella pulvinaat (Hook.et Grev.) Maxim. Selaginella contains a variety of flavonoids, including sotetsuflavone, amentoflavone, hinokiflavone, isocryptomerin, cryptomerin B , apigenin (apigenin), etc. Among them, the apigenin, also known as Amantuo biflavone, exists in many kinds of traditional Chinese medicines such as licorice, ginkgo leaf, and Hypericum perforatum, and the content of apigenin in Selaginella is much higher in other Chinese herbal medicines. Biflavonoids have antibacterial properties, can improve blood circulation, control dermatitis, control hair growth and regulate blood circulation. [0003] The amiflavones of Suicha spica were detected according t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 易铭杨永安章海燕唐希灿季浩
Owner JIANGSU TIANSHENG PHARMA
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