The invention discloses a new chiral separation method of a 2,3-diphenylpropionic acid single enantiomer, i.e., a method for synthesizing the 2,3-diphenylpropionic acid single enantiomer by performing enzyme-catalyzed enantioselective hydrolysis on a 2,3-diphenylpropionic acid enantiomer. 2,3-diphenylpropionate enantiomer is hydrolyzed by utilizing high selectivity and high catalysis efficiency of Candida antarctica lipase A and a solubilization effect of cyclodextrin on a 2,3-diphenylpropionate enantiomer is utilized, so that hydrolysis reaction of the 2,3-diphenylpropionate enantiomer in a phosphate buffering solution is enhanced; the conversion rate of a substrate and the optical purity of a product are respectively up to 44.79 percent and 98.24 percent, and the stereoselectivity E is greater than 276. According to the method disclosed by the invention, the problems of low optical purity, low yield, environment pollution and the like in a general separation technique are overcome; by adopting the method, the high conversion rate and the high selectivity required by hydrolysis of the 2,3-diphenylpropionate enantiomer can be realized, so that the aims of no-toxicity and no-harmlessness of a separation chiral compound, mild reaction conditions, simpleness of equipment, convenience in operation, low cost and the like are fulfilled.