Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

52results about How to "Strong throughput" patented technology

Non-key signature infrastructure building method based on block chain

InactiveCN107171812AStrong throughput and scalabilitySmall storage and network load requirementsUser identity/authority verificationDigital data protectionChain networkBlockchain
The invention provides a non-key signature infrastructure building method based on a block chain. The method comprises the steps that a core layer node is used to embed a root hash value of an aggregation layer into an OP_RETURN transaction script through identity authentication; broadcasting is carried out in the block chain network; packaging into the block is carried out to form a KSI Calendar hash account book; KSI release layer architecture based on the block chain is provided; a miner plays the role of release node in KSI; transaction information in the block chain network is collected; a Merkle tree is built; the release node simply needs to extract contents in OP_RETURN in transaction for Merkle tree building, so as to finally form CRH; and finally CRH is written into the block head. According to the invention, without changing the current non-key signature infrastructure architecture, the block chain is used to complete identity authentication of the KSI core layer node; the public transparency of the Calendar hash account book is protected; and the problems of long CRH release cycle, poor release media credibility and difficult recognition and processing in the prior art are solved.
Owner:光载无限(北京)科技有限公司

CYP2C19 gene polymorphyism detection kit and detection method thereof

The invention discloses a CYP2C19 gene polymorphyism detection kit and a detection method thereof. The kit comprises ultrapure water, a solution X, a 10*PCR (polymerase chain reaction) buffer solution, PCR primers, a 25mM magnesium chloride solution, DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the PCR primers include a different genotypic forward and reverse amplification primer, an internal DNA reference forward and reverse amplification primer and an internal reaction reference forward and reverse amplification primer on three SNP (single nucleotide polymorphism) sites of a CYP2C19 gene; and gene sequences of the primers are shown as SEQ ID NO. 1-NO. 13 (sequence identifier number 1-number 13). The detection method comprises the steps of collecting a sample, extracting nucleic acid, taking nucleic acid of a patient as a template to conduct PCR reaction, and using a GeXP genetic analyzer to conduct capillary electrophoretic separation on the sample. The detection kit and the detection method have the advantages of high specificity, high sensitivity, high flux, high reliability, low cost and no false negative results.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Primer composition for guiding nitroglycerin medication and healthy drinking, multiple gene detection kit and use method of kit

The invention discloses a primer composition for guiding nitroglycerin medication and healthy drinking, a multiple gene detection kit and a use method of the kit, and the kit comprises the primer composition, a PCR buffer solution and a positive reference substance, and the PCR buffer solution comprises ultrapure water, an X solution, a 10*PCR (polymerase chain reaction) buffer solution, a PCR primer, a 25mM magnesium chloride solution and DNA polymerase, the primer composition comprise two forward and reverse amplification primers of different gene types on the 2 SNP sites of genes related to the nitroglycerin medication and healthy drinking and forward and reverse amplification primers capable of reflecting internal reference, the gene sequences of the primers are represented as SEQ ID NO.1-NO.8; the use method comprises the step of acquiring a sample and extracting nucleic acid, the step of performing the PCR reaction by using extracted nucleic acid as a template, and the final step of separating the sample through capillary electrophoresis. The primer composition has the advantages of being strong in specificity, high in accuracy, high in flux, strong in reliability, low in cost and free from false negative result.
Owner:NINGBO HEALTH GENE TECHNOLOGIES CO LTD

Gene probe for simultaneously detecting five food-borne pathogenic bacteria through liquid chip and detection method

The invention relates to the technical field of security detection of agricultural and sideline products, in particular to a primer for simultaneously detecting five food-borne pathogenic bacteria through a liquid chip and a detection method. The detection method comprises steps as follows: a specific primer and a gene probe for five foodborne pathogenic bacteria are designed; DNA templates of the detected five foodborne pathogenic bacteria are prepared; PCR (polymerase chain reaction) amplification is performed on the five foodborne pathogenic bacteria; coupling is performed on the gene probe for the five foodborne pathogenic bacteria and microspheres, and a microsphere probe is obtained and verified; an obtained PCR amplification product and the microsphere probe have a hybridization reaction; a fluorescence signal of a hybridized product is detected, and quantitative analysis is performed on the five foodborne pathogenic bacteria. A method capable of simultaneously detecting five common foodborne pathogenic bacteria is successfully established, the variable coefficient is within 2%, the specificity is 100%, the sensitivity can reach 100 CFU/mL, and the method is equivalent to a fluorescent PCR technology and can be applied to high-throughput rapid detection of clinic and environment specimens of foodborne diseases.
Owner:GUANGZHOU CENT FOR DISEASE CONTROL & PREVENTION (GUANGZHOU HYGIENE INSPECTION CENT GUANGZHOU CENT FOR FOOD SAFETY RISK SURVEILLANCE & ASSESSMENT INST OF PUBLIC HEALTH OF GUANGZHOU MEDICAL UNIV)

Method for detecting trace metal elements in coke and application thereof

The invention discloses an extraction method of trace metal elements in coke and an application. The extraction method comprises the following steps: taking coke to prepare a sample, uniformly stirring the sample, weighing the sample, putting the sample into a beaker, adding deionized water, performing uniform stirring, and standing for a plurality of hours; filtering the soaked coke soak solutionto ensure that no visible particulate matters exist in the filtrate; adding the filtrate into a volumetric flask, fixing the volume, adding a nitric acid solution, and performing uniform mixing to obtain a first to-be-detected solution; and drying the filter residues, performing ashing, carrying out mixed acid digestion treatment to obtain a second to-be-detected solution; and finally, respectively measuring the content of the target metal element by adopting an EDTA method and an inductively coupled plasma atomic emission spectrometry. The method can extract the metal elements in the coke, has the advantages of simple steps, short time consumption and high extraction efficiency, and can eliminate experiment interference by adopting deionized water.
Owner:YANGCHUN NEW STEEL CO LTD

Method for detecting residual iron content in degreasing agent

The invention relates to a method for detecting the residual iron content in a degreasing agent. In the prior art, the residual iron content in the used degreasing agent cannot be accurately detected.A purpose of the present invention is mainly to solve the technical problem in the prior art. The technical scheme is that the method comprises: preparing a to-be-detected solution, wherein a certainamount of a to-be-detected degreasing agent sample solution is sucked and placed into a conical flask, a certain proportion of a hydrochloric acid solution is injected into the conical flask, the to-be-detected degreasing agent sample solution is subjected to acidification treatment, the acidified to-be-detected degreasing agent sample solution is transferred into a 100 ml volumetric flask, volume metering is performed to achieve a certain scale, and uniform shaking is performed to obtain the to-be-detected solution; determining the spectral intensity of iron in the to-be-detected solution byusing an inductively coupled plasma emission spectrometer; and calculating the residual iron content in the to-be-detected degreasing agent sample solution. According to the present invention, the method has advantages of high detection data accuracy, simple operation and low labor intensity, and can perform the detection and judgment on the actual cleaning effect of the cold rolling degreasing agent used in the production line.
Owner:SHANGHAI MEISHAN IRON & STEEL CO LTD

Real-time vehicle speed monitoring method based on flink architecture

The invention discloses a vehicle speed monitoring method based on a flink architecture, and the method comprises the steps of collecting data through a radar tachymeter, carrying out the data cleaning through a Flink platform, analyzing the data that the vehicle speed is greater than a speed limiting threshold value and the distance between vehicles is less than 200 meters through the flink platform, and obtaining the vehicle driving information; performing message consumption by using the flink platform, feeding overspeed vehicle information in the driving safety range back to the vehicle-mounted system, and enabling the vehicle-mounted system of the vehicle to report the overspeed vehicle condition to a driver; and meanwhile, performing data storage, so that historical data can be read,an overspeed accident investigation basis can be provided for traffic polices, and overspeed accidents can be simulated.
Owner:ZHEJIANG UNIV OF TECH

Solid flux culture device and application thereof

The invention relates to a solid flux culture device and application thereof, and belongs to the technical field of solid culture fermentation. The solid flux culture device comprises a culture plate and a plate cover arranged on the culture plate, wherein the culture plate comprises a culture plate body; a plurality of culture holes are formed in the culture plate body; multilayer gauze matched with the dimension of the culture hole is arranged at the inner bottom of each culture hole; a first through hole is formed in the middle of the bottom of each culture hole; a Luer joint matched with the first through hole is inserted in the first through hole in a penetrating way; a connector of the Luer joint is positioned at the outer part of the culture plate; a plurality of second through holes are formed in the plate cover; the distribution position of the second through hole is consistent with that of the culture holes. The solid flux culture device uses a porous culture plate; the culture flux can be realized on the solid culture medium; the flux screening culture is performed on products with advantages; the obvious directivity is realized.
Owner:FOSHAN HAITIAN FLAVOURING & FOOD CO LTD +2

Ultrasonic extraction atomization auxiliary carbon fiber ionization device and method of using same to realize ionization

The invention discloses an ultrasonic extraction atomization auxiliary carbon fiber ionization device and a method of using the same to realize ionization. The device includes a carbon fiber ion source and a mass spectrometry sample input channel. A sampling and ionization end of the carbon fiber ion source is located in front of a port of the mass spectrometry sample input channel. An ultrasonicextraction atomization device is arranged near the sampling and ionization end of the carbon fiber ion source. The method of using the ultrasonic extraction atomization auxiliary carbon fiber ionization device of the invention to realize ionization includes the operations of: placing a sample, which contains an extraction solvent, on the ultrasonic extraction atomization device, then sequentiallycarrying out extraction and atomization on the sample by the ultrasonic extraction atomization device, and then ionizing the atomized sample by the carbon fiber ion source. The ultrasonic extraction atomization auxiliary carbon fiber ionization device provided by the invention has good an ionization effect on peptide compounds, and can qualitatively and quantitatively analyze complex samples without the need for preprocessing the to-be-tested samples.
Owner:SHANGHAI INST OF ORGANIC CHEM CHINESE ACAD OF SCI

Novel electro-catalytic composite membrane material and preparation method thereof

The invention relates to a electro-catalytic composite membrane material and a preparation method thereof. The novel electro-catalytic composite membrane material is characterized in that the material can couple electro-catalytic technology and membrane separation technology. The preparation method comprises the following steps: taking a microporous separation membrane with electric conductivity, namely a carbon membrane, as a substrate, adopting a sol-gel method, mixing a metallic compound, water, a solvent, a catalyst, other additives and the like to be configured into a precursor solution, and obtaining an electro-catalytic composite membrane by sol ageing, film coating, drying and heat treatment; and preparing the composite membrane with superior performances by pretreating a substrate and controlling the mixture ratio of raw materials, the soakage time, the pulling speed, the sintering temperature and the like. The novel electro-catalytic composite membrane material takes the electro-catalytic composite membrane as an anode and a metallic material as a cathode to treat wastewater and realizes the reinforcement of the membrane separation process and the self-cleaning function under the action of a low-voltage electric field. The electro-catalytic membrane material has the advantages of low energy consumption, high separation efficiency, strong antifouling property and the like, and can be widely used for treating and recycling heavily polluted wastewater such as oily wastewater, dye wastewater, paper-making wastewater and the like. The preparation method has the advantages of simple preparation process, cheap equipment, easily-obtained raw materials, convenient operation and suitability for industrialized implementation.
Owner:DALIAN HUAXINYUAN TECHNOLOGY DEVELOPMENT LIMITED COMPANY

Super all-weather automatic diagnosis and treatment system

InactiveCN108648820AEasy to manageRealize automated round-the-clock diagnosis and treatmentMedical automated diagnosisHealthcare resources and facilitiesDiseaseMedicine
The invention discloses a super all-weather automatic diagnosis and treatment system, and belongs to the field of high and new technology medical treatment. According to the system, the contemporary mature and under mature high and new computer technology and all the links including medical field reception, diagnosis, treatment and nursing are combined with the personal lifetime information of thecitizens to be implemented to design a large system so as to integrate into a truly ultra-modern hospital. All-weather, automatic and standardized diagnosis and treatment of all the diseases incorporated into the system can be realized. With application of the system, the old medical mode can be completely broken through and the medical field can enter a brand-new era. The throughput is high, thepracticability is high, the cure rate is high, the accident rate is low, the hospital management can be strengthened, the medical procedures can be standardized, the human error accident can be reduced, the medical quality can be enhanced, the corruption can be eliminated, the situation of rushing about see a doctor can be omitted for the patient, the working efficiency can be enhanced, the medical environment can be purified, the medical cost can be reduced, the burden of the country can be reduced, the major social problem of medical difficulties can be truly solved and thus the system hasgreat social and economic significance.
Owner:河南省金拐杖医疗科技有限公司

Oral liquid used for zinc supplementation, and preparation method thereof

The invention discloses an oral liquid used for zinc supplementation, and a preparation method thereof, and belongs to the technical field of traditional Chinese medicine health product. The oral liquid used for zinc supplementation comprises ingredients, which can be taken as both health care products and drugs, such as pseudostellaria root, prepared rehmannia root, Radix Polygoni Multiflori Preparata, and bighead atractylodes rhizome, and ingredients, which can be taken as both food and drugs, such as licorice, Poria cocos, and dried orange peel. Pseudostellaria root is taken as the sovereign drug, Radix Polygoni Multiflori Preparata is taken as the ministerial drugs, bighead atractylodes rhizome, Poria cocos, dried orange peel, and prepared rehmannia root are taken as the conductant drugs; licorice is adopted for harmonizing the above drugs. The preparation method comprises four steps including smashing, decocting, single syrup preparation, and oral liquid preparation. The preparation method is capable of extracting the effective components fully, avoiding destroy of the effective components caused by long term decoction. The oral liquid is composed of the traditional Chinese medicine components which can be taken as both health care products and drugs, or food and drugs, no antiseptic is added, traditional Chinese medicine concepts and syndrome differentiation and treatmentare taken as the principles; the oral liquid is capable of nourishing both spleen and kidney, nourishing both Qi and blood, strengthening the spleen in transportation, improving digestion functions,adjusting internal organs functions, realizing natural zinc supplementation without supplying zinc, and adjusting the integral internal organs functions.
Owner:福建天人药业股份有限公司

High-throughput quantitative detection kit for diarrhea pathogenic bacteria

The invention discloses a high-throughput quantitative detection kit for diarrhea pathogenic bacteria. The kit is characterized in that the kit includes a LAMP detection primer set, whose gene sequences are shown as SEQ ID NO.1-NO.36, of 9 kinds of diarrhea pathogenic bacteria; and the kit also includes a sample template, 10*reaction buffer, MgSO4, dNTP, Bst DNA polymerase and double distilled water. The kit has the advantages of being strong in specificity, high in sensitivity, high in throughput, strong in reliability and low in cost, and has no false negative results.
Owner:浙江正合谷生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products