The invention discloses fluorescent materials with an aggregation-induced emission effect and circular polarization and a preparation method of the fluorescent materials. The general structural formula of the fluorescent materials is shown in the description, wherein R represents any one of H, phenyl, C1-C2 alkoxy substituted phenyl, C1-C3 alkyl substituted phenyl, trifluoromethyl substituted phenyl, thienyl, C1-C3 alkyl substituted thienyl and triphenylamine. After a ring-locking o-dinaphthol intermediate is synthesized from o-dinaphthol and 1,6-diiodohexane, a coupled product is synthesizedthrough a Suzuki coupling reaction, finally, an aldehyde group and malononitrile are subjected to a reaction, and the fluorescent materials are obtained. The fluorescent materials realize aggregation-induced emission characteristic and chirality in one molecular framework and can prevent quenching of circular dichroism spectrum, namely, the AACD effect, and by use of the two properties, microcrystals with different morphologies are obtained by self-assembly of the synthesized fluorescent materials, so that the compounds realize spectrum-adjustable circularly polarized light.