The invention discloses a bletilla striata tissue culture seedling transplantation and acclimation technology, which comprises the following steps: 1, preparing sand soil, the sand soil being one of yellow sand soil and black sand soil, frying-heating the sand soil to more than 80 DEG C by virtue of a caldron to evaporate moisture in the sand soil and kill parasite ova, cooling the sand soil to room temperature, and screening the sand soil once with a fine screen for later use; 2, spreading the prepared sand soil into bletilla striata tissue culture seedling bottles which are about 5cm high, the spread sand soil having a thickness capable of just covering the roots of seedlings; 3, placing the seedling bottles in step 2 in a tissue culture room to grow for 15 to 30d, and keeping the room temperature of 20 to 30 DEG C and the humidity of about 70 percent. According to the technology, the tissue culture seedlings are not required to be cleaned in an acclimation process, so that the roots of the seedlings are prevented from damage, meanwhile, manpower, chemicals and time are greatly saved, and complex steps for tissue culture seedling acclimation are simplified.