The invention provides mulberry, colla corii asini and ginseng wine which is prepared from the following raw materials in parts by weight: 70 to 80 parts of mulberry fruit wine, 4 to 8 parts of honey, 2 to 4 parts of cinnamon, 3 to 6 parts of ginsengs, 5 to 10 parts of lycium barbarum, 4 to 8 parts of raspberry, 4 to 8 parts of longan, 4 to 8 parts of red dates, 3 to 6 parts of lotus leaves, 4 to 8 parts of rhizoma polygonati, 4 to 8 parts of colla corii asini, 3 to 5 parts of lily flowers, 4 to 8 parts of pollen pini, 4 to 8 parts of chrysanthemum flowers, 3 to 6 parts of embelia laeta, 5 to 10 parts of rock candies and 50 to 80 parts of yellow wine. The invention further provides a preparation method of the mulberry, colla corii asini and ginseng wine. The mulberry, colla corii asini and ginseng wine provided by the invention has the effects of nourishing yin and blood, invigorating spleen, supplementing qi, tonifying kidney, warming meridians, clearing away toxic substances, moistening dryness, enhancing the immunity of a human body, improving eyesight, soothing the nerve, resisting aging and oxidization, maintaining beauty and keeping young, and is health wine drinkable for all types of people.