The invention discloses Lactobacillus fermentum suo for adjusting intestinal tract motion and preventing constipation and a use thereof. The Lactobacillus fermentum suo has a preservation number of CCTCC NO: M2013511, has strong acid resistance, has a survival rate of 92.46+ / -4.06% after being arranged in an artificial gastric juice with a pH value of 3.0 for 3h, can slowly grow in cholate with a concentration of 1.0%, has a growth rate 17.36+ / -1.19% that of cholate-free culture, has cell hydrophobicity of 68.44+ / -2.48% and can normally grow in the human intestinal tract. Lactobacillus fermentum suo can relieve constipation-caused mouse weight reduction, reduce tarry stool excretion time, improve a small intestine propulsion rate, improve serum MTL, Gas, ET, AchE, SP and VIP factor levels to different degrees, reduce a SS factor, improve food intake and water intake, relieve reduction of a stool excretion mount, the number of stool particles and stool water content and can produce a certain constipation prevention effect similar to those of constipation treatment drugs.