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Lactobacillus fermentum Lee working leavening agent product and constipation-prevention and health application thereof

A technology of working starter, Lactobacillus fermentum, applied in the field of microbiology

Inactive Publication Date: 2015-03-25
SOUTHWEST UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents or patent applications do not fully relate to microorganisms that have the properties of regulating intestinal motility, preventing constipation, etc.

Method used

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  • Lactobacillus fermentum Lee working leavening agent product and constipation-prevention and health application thereof
  • Lactobacillus fermentum Lee working leavening agent product and constipation-prevention and health application thereof
  • Lactobacillus fermentum Lee working leavening agent product and constipation-prevention and health application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Isolation, purification and preliminary identification of Lactobacillus fermentum Lee This example was carried out according to the following steps:

[0048] (1) Separation and purification of Lactobacillus fermentum Lee

[0049] Aseptically draw 500 μL of the sample, add it to 5 mL of sterile physiological saline and mix it to make a 1:10 uniform dilution, and continue to make a certain proportion of dilution. Select the appropriate gradient of the dilution solution, use a sterile pipette tip to draw 100μL each and apply it to the MRS solid plate medium, incubate at 30℃ for 48-72h, observe and record the colony morphology ( figure 1 ). Use an inoculating loop (or sterilized toothpick) to pick different colonies from the surface and inside of the plate and inoculate them in the MRS liquid medium. Place them on a 30℃, 300r shaker for 24-48h; repeat the above steps for continuous activation for 2 generations and then smear leather Ran's staining microscopy ( figur...

Embodiment 2

[0054] Example 2: In vitro screening of Lactobacillus fermentum Lee

[0055] (1) Screening of probiotics tolerating pH3.0 artificial gastric juice

[0056] The preparation of artificial gastric juice: NaCl 0.2%, pepsin 0.35%, adjusted to pH 3.0 with 1M HCl, and sterilized by vacuum pump in a sterile operating table for use.

[0057] Determination of the tolerance of probiotics to artificial gastric juice: Take 5mL of the activated strain culture solution, pour it into a sterilized 10mL centrifuge tube in a sterile operating table, collect the bacteria by centrifugation at 3000r / min for 10min, add 5mL for sterilization Mix physiological saline to make a bacterial suspension. Take 1 mL of the bacterial suspension and 9 mL of pH3.0 artificial gastric juice, shake it, place it in a constant temperature shaker (37℃, 300r), and sample at 0h and 3h. Pour MRS agar medium at 37°C and cultivate for 48h. Measure the number of viable bacteria with the plate counting method, and calculate the s...

Embodiment 3

[0070] Example 3: Active carbon induced constipation experiment

[0071] Fed healthy Kunming mice, 6 weeks old, weight 23±2g, female, 60 mice. After feeding the basic diet for 5 days, the mice were randomly divided into 5 groups according to their body weight, namely: normal group, constipation control group, 5% RS3 group, 10% RS3 group and 15% RS3 group, each with 12 mice. Raised in a stainless steel cage, the room temperature is maintained at 24±2°C, the relative humidity is 50±10%, and the light and dark are rotated for 12 hours (8:00-20:00 lighting). Throughout the experimental period, the normal group and the constipation control group were fed with basic feed. The mice in the experimental group were fed with a daily dose of 1.0×10 9 CFU / kg gavage probiotics. After 2 weeks, the mice in the 5 groups except the normal group were given a 10% concentration of 2℃ activated carbon ice water, 0.2mL / mouse, once a day, for 3 consecutive days. Until the last day of the experiment, a...

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Abstract

The invention discloses a lactobacillus fermentum Lee working leavening agent product and constipation-prevention and health application thereof. The preservation number of lactobacillus fermentum Lee in China Center for Type Culture Collection is CCTCCM 2013512. The lactobacillus fermentum Lee can normally grow in intestinal tracts of human bodies. A mouse in vivo test further shows that the lactobacillus fermentum Lee can be used for retarding a mouse weight-reducing tendency caused by constipation; melena defecation time is accelerated and the small intestine propelling rate is improved; levels of MTL, Gas, ET, AchE, SP and VIP factors of blood serum can be increased to different extents, and SS factors are reduced; the food intake and the water drinking amount are increased; the tendency that defecation amount, the defecation grain quantity, and the moisture content of excrement decline are reduced; and the effect similar with constipation drugs is realized and a certain constipation-prevention effect is represented. The optimal use dosage is 1.0*10<9>CFU per kilogram of weight.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, more specifically, the present invention relates to a kind of Lactobacillus fermentum that can regulate intestinal movement and prevent constipation and use the bacterial strain as a starter Or added fermented milk, health food and animal health products. Background technique [0002] Lactobacillus is closely related to human life and is one of the beneficial microorganisms widely used in food fermentation, industrial lactic acid fermentation and medical care. Many of the now recognized Lactobacillus and Bifidobacterium genera have specific favorable properties and genetic traits, showing extremely strong probiotic properties. Lactobacillus can not only improve the nutritional value of food, improve the flavor of food, prolong the storage time, but also improve the functional properties of food. It can improve the digestibility and biological value of food and promote digestion a...

Claims

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Application Information

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IPC IPC(8): A23C9/123A23C9/127
Inventor 李键索化夷骞宇赵欣陈炼红兰道亮谢婕
Owner SOUTHWEST UNIVERSITY FOR NATIONALITIES
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