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Lactobacillus paracasei CCFM1069 and application thereof

A Lactobacillus and paracheese technology, applied in the field of microorganisms, can solve the problems of non-long-term use, side effects, etc., and achieve the effects of strong adsorption capacity, alleviation of toxicity, and improvement of fecal water content

Active Publication Date: 2020-05-08
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drugs currently used to treat constipation are mainly divided into volumetric laxatives, osmotic laxatives, stimulant laxatives, and lubricating laxatives, but drug treatments have certain side effects and cannot be used for a long time

Method used

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  • Lactobacillus paracasei CCFM1069 and application thereof
  • Lactobacillus paracasei CCFM1069 and application thereof
  • Lactobacillus paracasei CCFM1069 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1: Lactobacillus paracasei CCFM1069 has good tolerance to simulated gastrointestinal fluid

[0080] The cryopreserved Lactobacillus paracasei CCFM1069 was inoculated in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), cultured anaerobically at 37°C for 48 hours, and subcultured in mMRS medium for 2-3 hours. After the second time, take 1mL of the culture solution of Lactobacillus paracasei CCFM1069, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (containing 1% pepsin, mMRS medium at pH=2.5), and culture it anaerobically at 37°C. Samples were taken at 0h, 0.5h, 1h, and 2h, and the mMRS agar medium was poured to culture the plate colonies, and the number of viable bacteria was determined and the survival rate was calculated.

[0081] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solu...

Embodiment 2

[0086] Embodiment 2: Lactobacillus paracasei CCFM1069 has no toxic and side effects to BALB / C mice

[0087] Suspend Lactobacillus paracasei CCFM1069 bacterium in 2% sucrose solution to make a concentration of 3.0 × 10 9 CFU / mL bacterial suspension. Eight healthy male BALB / C mice with a body weight of about 16-20 g were taken, and after one week of adaptation to the environment, they were given the bacterial suspension of this concentration by intragastric administration once a day, observed for one week, and the death and body weight were recorded.

[0088] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 9 CFU / mL of Lactobacillus paracasei CCFM1069 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0089] Table 3 Changes in body weight and death of mice

[0090]

[0091] Note: -: no death of mice

Embodiment 3

[0092] Embodiment 3: Effect of Lactobacillus paracasei CCFM1069 on the first black stool of constipation mice

[0093] Take Lactobacillus paracasei CCFM1069 out of the refrigerator at -80°C, streak on the MRS plate, culture at 37°C for 48 hours, pick a single colony in the MRS liquid tube, culture at 37°C for 18 hours, and inoculate at 2% volume In the new MRS liquid medium, culture at 37°C for 18h, culture for another generation in the same way, then centrifuge the lactic acid bacteria suspension at 8000r / min, 4°C for 8min, and then re-infect with 3% sucrose solution. Suspended to obtain a bacterial suspension, which was stored in a -80°C refrigerator.

[0094] Take 30 6-week-old healthy male Balb / C mice, acclimate to the environment for 1 week, and divide them into 5 groups randomly: blank control group (NC), model control group (M), phenolphthalein control group (RH), para-cheese milk Bacillus CCFM1069 intervention group (CCFM1060), Lactobacillus paracasei 4-1 (4-1), each ...

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Abstract

The invention discloses lactobacillus paracasei CCFM1069 and an application thereof. The lactobacillus paracasei CCFM1069 can bear stomach intestine environment of human bodies, notably increase watercontent of excrement and urine, increase the propelling rate of small intestines, shorten the time of discharging a first granule of black stool, reduce the content of peptide YY in serum, raise thelevel of 5-hydroxytryptamine in colon tissue, raise the level of nerve nutrition factors 3 in the colon tissue, reduce Aqp4 genetic transcription level in the colon tissue, increase the number of Cajal interstitial cells in the colon tissue, and increase the content of short chain fatty acids in intestinal tracts; and the lactobacillus paracasei CCFM1069 has high adsorption capacity on pentadecafluorooctanoic acid, has the capacity of relieving PFOA toxicity and has quite good application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Lactobacillus paracasei CCFM1069 and application thereof. Background technique [0002] At present, constipation has become one of the main problems plaguing modern people, which affects the emotions of patients and reduces their quality of life and life satisfaction. Many patients are not very satisfied with traditional treatment options, mainly because of their therapeutic effects. Poor. Therefore, present patients and ordinary normal people need an effective method to treat and prevent constipation. From clinical observation, female patients are more numerous than male patients. Among constipation patients in North America, the ratio of females to males is about 2.2:1, and the probability of constipation increases with age, especially after the age of 65. Domestic surveys also show that the occurrence of chronic constipation has obvious regional character...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A23L33/00A23C9/123A23C11/10A23L11/00A23C13/16A23C19/032A61K35/747A61P1/10C12R1/225A23L11/50A23L11/60
CPCA23L33/135A23L33/00A23C9/123A23C11/106A23C13/16A23C19/0323A61K35/747A61P1/10A23V2200/00A23L11/50C12R2001/20C12N1/205C12R2001/225A23V2400/165A23V2200/3204
Inventor 王刚陈卫杨树荣赵建新张灏
Owner JIANGNAN UNIV
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