A strain of Bifidobacterium longum and its application

A technology of bifidobacteria longum and bifidobacteria is applied in the field of microorganisms to achieve the effects of strong adhesion, improved intestinal propulsion rate, and good growth and acid production capacity.

Active Publication Date: 2019-11-08
上海华朴生命健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been researches at home and abroad involving bifidobacteria to relieve or treat constipation, but mainly in the form of mixed bacteria and synbiotics, and only a single strain is used for research. In addition, due to individual differences and differences between different strains difference, there is still a certain probability that bifidobacteria can relieve constipation

Method used

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  • A strain of Bifidobacterium longum and its application
  • A strain of Bifidobacterium longum and its application
  • A strain of Bifidobacterium longum and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Example 1: Screening of Bifidobacterium longum CCFM 760

[0039] 1) Use a disposable sterile toilet to collect several parts of fresh feces from a two-year-old girl in Binhu District, Wuxi City, Jiangsu Province, put the feces samples in a modified MRS liquid test tube with fructooligosaccharides as the carbon source, and place them in an anaerobic workstation ( N 2 :CO 2 :H 2 =80:10:10) to carry out the enrichment of bifidobacterium strains within 12h;

[0040] 2) Perform gradient dilution with sterile normal saline, use a pipette to draw 100 μL of the corresponding dilution sample and spread it on the modified MRS solid plate containing 0.02g / mL calcium carbonate, and incubate anaerobically for 24-48h;

[0041] 3) Pick a single colony with calcium carbonate calcium dissolution circle, and single colony that conforms to the basic form of lactic acid bacteria is streaked and separated on the improved MRS solid medium, and the streak is repeated 3 times;

[0042] 4) P...

Embodiment 2

[0046] Embodiment 2: the preparation of Bifidobacterium longum CCFM760 bacterial suspension

[0047] Experimental method: Inoculate the 3-generation activated bacterial solution into 1L of the modified MRS medium at an inoculum size of 2%, shake and mix well, and culture anaerobically at 37°C for 20 hours. Centrifuge at 5000 rpm and 4°C for 15 minutes, discard the supernatant, wash twice with sterile saline, centrifuge under the same conditions, and resuspend with 15 mL of sterile 30% sucrose water. After oscillating evenly, measure the number of viable bacteria with the plate pouring method, and store in a -80°C refrigerator for 1 week. Among them, the formula of the improved MRS medium is: beef extract 10g; tryptone 10g; yeast powder 5g; glucose 20g; anhydrous sodium acetate 5g; 4 ·7H 2 O 0.1g; MnSO 4 ·H 2 O 0.05g; Diammonium hydrogen citrate 2g; K 2 HPO 4 ·3H 2 O 2.6g; Tween-801mL; L-cysteine ​​hydrochloride 1g; adjust the pH to 6.8±0.2; dilute to 1L. Sterilize at 1...

Embodiment 3

[0049] Example 3: Relief effect of Bifidobacterium longum CCFM760 on constipation in mice

[0050] Experimental method: The CCFM760 bacterial suspension with the function of relieving constipation in Example 1 was used as the experimental group, and Bifidobacterium animalis BB12 and phenolphthalein were used as the positive controls, and a comparative experiment was carried out on normal mice and constipation mice respectively. The effect of Bifidobacterium longum CCFM760 on alleviating constipation in mice was evaluated by comparing the water content of feces, the time of first black stool discharge, the propulsion rate of small intestine, the content of constipation-related gastrointestinal peptide in colon tissue sections and serum of mice in each group.

[0051] Take 25 healthy BALB / c male mice (7 weeks old) weighing 22-26g, and divide them into 5 groups randomly (normal control group, constipation model group, CCFM760 experimental group, BB12 control group, phenolphthalein...

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Abstract

The invention discloses a strain of bifidobacterium longum and application thereof, belonging to the technical field of microorganisms. The Bifidobacterium longum of the present invention has been preserved in the General Microbiology Center of the China Microbial Strain Preservation Management Committee on December 7, 2017. The preservation number is CGMCC NO.15032, and the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing. Institute of Microbiology, Chinese Academy of Sciences. The Bifidobacterium longum strain of the present invention has good growth, acid production and acid resistance and strong adhesion ability, can significantly improve the feces water content and small intestinal propulsion rate of mice with constipation, shorten the time for the first black stool, and relieve the colonic mucosa. Inflammation, regulates the content of constipation-related gastrointestinal peptides in serum, and its effect of relieving constipation is equivalent to that of Bifidobacterium animalis BB12, but the effect of small intestinal propulsion rate is better than that of Bifidobacterium animalis BB12 and phenolphthalein. Therefore, the Bifidobacterium longum strain CCFM760 provided by the present invention can be widely added to various food or drug matrices and used.

Description

technical field [0001] The invention relates to a bifidobacterium longum and application thereof, belonging to the technical field of microorganisms. Background technique [0002] The prevalence of constipation increases sharply with age. Surveys have found that the proportion of constipation in the elderly in Western countries is 40%, and it is as high as 70% in nursing homes; the situation in my country is slightly better, with 11.9% of elderly patients with constipation and 45.53 in nursing homes. %. Long-term difficulty in defecation can induce or aggravate other diseases such as hemorrhoids, prostatic hypertrophy, myocardial infarction or cerebrovascular disease, etc., and even life-threatening in severe cases, becoming a public health problem. [0003] The causes of constipation include genetic factors, psychological factors, dietary factors, abnormal defecation dynamics, abnormal regulatory factors such as hormones and neurotransmitters, and intestinal flora imbalance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P1/10A23L33/135C12R1/01
CPCA61K35/745A61K2035/115A23L33/135C12N1/205C12R2001/01
Inventor 陈卫张秋香应聪萍张灏赵建新田丰伟王刚翟齐啸于雷雷
Owner 上海华朴生命健康科技有限公司
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