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Lactobacillus paracasei ccfm1069 and its application

A Lactobacillus and paracheese technology, applied in the field of microorganisms, can solve the problems of side effects and long-term use, and achieve the effects of strong adsorption capacity, alleviation of toxicity, and improvement of small intestinal propulsion rate.

Active Publication Date: 2022-05-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The drugs currently used to treat constipation are mainly divided into volumetric laxatives, osmotic laxatives, stimulant laxatives, and lubricating laxatives, but drug treatments have certain side effects and cannot be used for a long time

Method used

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  • Lactobacillus paracasei ccfm1069 and its application
  • Lactobacillus paracasei ccfm1069 and its application
  • Lactobacillus paracasei ccfm1069 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Example 1: Lactobacillus paracasei CCFM1069 has no toxic and side effects on BALB / C mice

[0080] The Lactobacillus paracasei CCFM1069 bacteria were resuspended in 2% sucrose solution to make a concentration of 3.0 × 10 9 CFU / mL bacterial suspension. Eight healthy male BALB / C mice with a weight of about 16-20 g were taken. After a week of acclimatization, the bacterial suspension of this concentration was given by gavage once a day, observed for one week, and the death and body weight were recorded.

[0081] The results showed that the feeding concentration was 3.0×10 9 Lactobacillus paracasei CCFM1069 at CFU / mL did not cause significant effects on mice, no significant changes in body weight, and no death. The mice showed no obvious pathological symptoms.

Embodiment 2

[0082] Example 2: Effect of Lactobacillus paracasei CCFM1069 on the first black stool in constipated mice

[0083] Lactobacillus paracasei CCFM1069 strain was taken out from the -80°C refrigerator, streaked on the MRS plate, incubated at 37°C for 48h, picked a single colony in the MRS liquid tube, incubated at 37°C for 18h, and inoculated with 2% volume. In a new MRS liquid medium, cultured at 37 °C for 18 h, and cultured for another generation in the same way, and then centrifuged the lactic acid bacteria suspension at 8000 r / min and 4 °C for 8 min, and then reconstituted with 3% sucrose solution. Suspended to obtain bacterial suspension, which was frozen at -80°C.

[0084] Thirty 6-week-old healthy male Balb / C mice were randomly divided into 5 groups: blank control group (NC), model control group (M), phenolphthalein control group (RH), and para-cheese milk. Bacillus CCFM1069 intervention group (CCFM1060), Lactobacillus paracasei 4-1 (4-1), each group contained 6 mice, the ...

Embodiment 3

[0090] Example 3: Lactobacillus paracasei CCFM1069 significantly improves the small intestine propulsion rate in constipated mice

[0091] Balb / C mice were grouped and modeled as in Example 1. On the 30th day, mice in each group were fasted and watered overnight. At 8:00 am on the 31st day, the blank group was given 0.2 ml of 3% sucrose solution, and both the model group and the bacteria perfusion group were given 0.2 ml of loperamide hydrochloride solution (10 mg / kg b.w). The four groups of bacteria gavage groups were gavaged with the ink containing their respective gavage contents. After 30 minutes, the mice were sacrificed, the abdominal cavity was opened, and the upper end was cut from the pylorus and the lower end to the cecum. The leading edge is the "ink advancement length", and the small intestine advancement rate is calculated according to the following formula.

[0092] Small intestine advancement rate (%)=(ink advancement length (cm)) / (total length of small intest...

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Abstract

The invention discloses Lactobacillus paracasei CCFM1069 and its application. Lactobacillus paracasei CCFM1069 can tolerate the gastrointestinal environment of human body, significantly improve the water content of feces, improve the propulsion rate of small intestine, shorten the time for the first black stool, and down-regulate casein in serum. Tyrosin content, up-regulation of serotonin level in colon tissue, up-regulation of neurotrophic factor 3 level in colon tissue, down-regulation of Aqp4 gene transcription level in colon tissue, increase of the number of interstitial cells of Cajal in colon tissue and increase of short-chain fatty acid content in intestine content; the Lactobacillus paracasei CCFM1069 has a strong adsorption capacity for perfluorooctanoic acid, has the ability to alleviate the toxicity of PFOA, and has a very wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to Lactobacillus paracasei CCFM1069 and applications thereof. Background technique [0002] At present, constipation has become one of the main problems plaguing modern people, which affects the mood of patients and reduces their quality of life and life satisfaction, and many patients are not very satisfied with the traditional treatment plan, mainly because of the treatment effect. Poor. So now patients and the general normal population need an effective method to treat and prevent constipation. From clinical observation, the number of female patients is higher than that of male patients. Among North American constipation patients, the number of women outnumbered men was about 2.2:1, and the probability of constipation increased with age, especially after the age of 65. Domestic surveys also show that the occurrence of chronic constipation has obvious regiona...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23L33/00A23C9/123A23L11/50A23L11/60A23C13/16A23C19/032A61K35/747A61P1/10C12R1/225
CPCA23L33/135A23L33/00A23C9/123A23C11/106A23C13/16A23C19/0323A61K35/747A61P1/10A23V2200/00A23L11/50C12R2001/20C12N1/205C12R2001/225A23V2400/165A23V2200/3204
Inventor 王刚陈卫杨树荣赵建新张灏
Owner JIANGNAN UNIV
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