31results about How to "Omit preprocessing" patented technology

The invention relates to the technical field of non-continuous enhanced metal-based composite materials, and in particular relates to an in-situ SiC-TiC particle mixing enhanced aluminum-based composite material and a preparation process thereof. For overcoming the defects of high preparation cost, complicated process and poor wettability and compatibility of particles and substrates in the prior art, the invention provides the in-situ SiC-TiC particle mixing enhanced aluminum-based composite material and the preparation process thereof. The preparation process comprises the following steps: firstly, preparing in-situ reaction system press cakes: dividing the designed press cakes into inner and outer layers, wherein the mass ratio of the inner layer to the outer layer is 3:10, and then pressing by a pressure machine; then, preparing Al-4.5Cu based alloy by taking pure Al and Al-50% Cu as raw materials; and finally adding the press cakes in Al-4.5Cu melt to carry out in-situ reaction. Compared with the prior art, the preparation method disclosed by the invention has the advantages that the in-situ produced SiC particles are fine and reach micron scale.

The present invention provides a primer pair for amplifying a target region containing a detection target site in the CYP2C19 gene using a gene amplification method, which can specifically amplify the region. Two primer pairs each including a forward primer containing the base sequences of SEQ ID NO: 12 and SEQ ID NO: 32 and a reverse primer containing the base sequences of SEQ ID NO: 22 and SEQ ID NO: 48 were used. By using this primer pair, two regions of the CYP2C19 gene containing the occurrence sites of two polymorphisms (CYP2C19 gene*2, CYP2C19*3) can be amplified simultaneously in the same reaction solution.

A double-transmission fluorescent chemical sensor takes an inner surface of a small glass bottle as a substrate and is a ratio fluorescent sensor formed by the substrate and quantum dots-porphyrin superstructure monox nano particles fixed on the substrate. The preparation method comprises the following steps: first, embedding green quantum dots into monox nano particles; then, coating red fluorescent porphyrin sensitive layer on the surface of the monox after the surface is subjected to amination in a covalence coupling manner, thereby obtaining the double-transmission quantum dot-porphyrin superstructure monox nano particles; and finally, processing the particles into the fluorescent chemical sensor. The sensor is convenient to carry and operate, can visually detect out 1ppm nitrogen dioxide gas pollutant in a real time and on-line manner, and has excitation wave having length of 370 nm.

The invention discloses a preparation method of polyethylenimine-modified ascorbic acid carbon nano-dots, comprising the steps of: adding polyethyleneimine into a reaction kettle containing ascorbic acid solution, and the molar ratio of polyethyleneimine to ascorbic acid is 0.05-1 : 1, heat to 180-220°C after sealing, react for 5-7h, cool and filter, dialyze for 6-10h, change ultrapure water every 1.5-2.5h, freeze-dry to obtain carbon nano-dot powder; and ascorbic acid carbon nano-dot Application of fluorescent probe in visual detection of hypochlorous acid. The probe of the present invention is sensitive to hypochlorous acid, and the detection limit is 14nmol / L; it also has high selectivity to hypochlorous acid, good stability, can be stored and used for a long time, and the synthesis is simple, and a portable hypochlorous acid has been developed The detection test paper realizes the purpose of simple, rapid and visual detection of hypochlorous acid, and is conducive to commercial promotion and application.

Disclosed is a primer set for amplifying a region in CYP2C9 gene that contains a detection target site having CYP2C9*3 therein by a gene amplification method, which enables to amplify the region specifically. A pair of primer set is used, which comprises a forward primer comprising a nucleotide sequence depicted in SEQ ID NO:4 and a reverse primer comprising a nucleotide sequence depicted in SEQ ID NO:17. The primer set enables to amplify a region containing a site having a polymorphism CYP2C9*3 occurring in CYP2C9 gene in a specific manner and with a high degree of efficiency.

The invention belongs to the field of analysis methods of radioactive elements, and particularly relates to a method for analyzing and detecting trace radioactive elements by a double-channel fluorescent uranyl ion probe. The probe is established on the basis of a semiconductor quantum dot-carbon quantum dot double-channel fluorescent signal with a method mainly comprising the following steps: (1)synthesizing a green fluorescent carbon quantum dot; (2) synthesizing a red fluorescent cadmium quantum dot; (3) preparing the probe. The detection principle is that uranyl ions can quench fluorescence of the red quantum dot, while the green fluorescence signal of the carbon quantum dot remains stable and unchanged, and quantitative detection of a to-be-detected sample is realized by testing thelinear relationship between concentration of the sample and the ratio of the red fluorescence signal to the green fluorescence signal. With adoption of the method, use of large-scale instruments can be avoided to a certain extent, all that is required is only one hand-held ultraviolet lamp for visual detection, operation is simple, convenient and quick, the pretreatment process is omitted, and high-sensitivity selective detection of the trace uranyl ions in a solution can be realized.

The invention relates to a double colour fluorescent chemical sensor used for visually detecting explosive, in particular to a paper-based or membrane-based sensor comprising a filter paper or glass slide substrate and double colour quantum dot superstructure silicon oxide nano particles uniformly distributed on the substrate. The method for manufacturing the sensor comprises the following steps of: firstly embedding red or green quantum dots into silicon oxide nano particles, aminating the surface of silicon oxide, then carrying out covalence coupling and coating a green or red quantum dot sensitive layer to obtain the double colour quantum dot superstructure silicon oxide nano particles, and finally processing to obtain the fluorescent chemical sensor. By applying the sensor, explosive residue with the trace of 5nanogram / mm<2> can be visually and rapidly detected online in real time. The fluorescence intensity of outer quantum dots is 2-10 times that of inner quantum dots.

The invention discloses a method for detecting mercapto boimolecules based on dually-emitted fluorescence of a zinc sulfide-doped nano crystal probe, which belongs to the technical field of chemical measurement. The method comprises the following steps: preparing zinc sulfide-doped nano crystals, modulating a dually-emitted fluorescence signal, establishing a standard curve, and detecting the mercapto biomolecules, wherein the standard curve is a curve of a correspondence between the variation of a dually-emitted fluorescent signal intensity specific value determined under the excitation of ultraviolet rays and a concentration of a substance to be detected, i.e. the standard curve which is established by successively adding a sample solution of the substance to be detected with a gradientconcentration into a zinc sulfide-doped nano crystal probe modulated via the spectrum with concentration being 1 to 100 microgrammes per milliliter under the excitationof the ultraviolet rays and gradually determining a specific value of the fluorescence intensity. By adopting the method, the interference of other impurities in the sample can be effectively avoided, the selectivity is good, a pretreatment process is omitted, and the sensitive detection for the mercapto biological active molecules such as glutathione, cysteine, homocysteine can be achieved.

A method for preparing a self-cooling and anti-closing lithium-ion battery diaphragm belongs to the technical field of battery diaphragms. A self-cooling and anti-closing film is obtained by coating a slurry layer on one side or both sides of a base film, and then drying it. Porous lithium-ion battery separator, the slurry layer is ceramic slurry or erythritol modified ceramic slurry, and at least one end surface of the base film is coated with erythritol modified ceramic slurry, and discloses A specific method for preparing erythritol modified ceramic slurry is provided. The preparation method in the present invention provides a stable and efficient process for the production of self-cooling and anti-closing lithium-ion battery separators. The prepared erythritol modified Ceramic slurry has both low shrinkage rate and high safety at high temperature of ceramic slurry.