The purpose of the present invention is to provide a medical material which has both safe cytotoxicity and efficient antibacterial and antiviral properties, a medical protective article, and a manufacturing method thereof. The multilayer copper-based zeolite fiber medical material has a four-layer structure which sequentially comprises an outermost layer, a first middle layer, a second middle layer and an innermost layer, wherein the outermost layer and the second middle layer are copper-based zeolite fiber layers, the first middle layer is a hydrophobic fiber layer, the innermost layer is a hydrophilic fiber layer, the porosity of the copper-based zeolite fiber layer on the outermost layer is greater than that of the hydrophobic fiber layer on the first middle layer; each copper-based zeolite fiber layer comprises copper-based zeolite and fiber yarn formed by winding fibers; the copper-based zeolite is independently dispersed on the surface of the fibers, and the mass of the copper-based zeolite is reduced in a gradient mode from outside to inside along the radial interface of the fiber line. The medical material and the prepared medical protective article have the two technical advantages of low cytotoxicity and high antibacterial and antiviral properties, have the effect of efficiently killing viruses (especially novel coronavirus) and enough biological safety, are expectedto rebuild the industrial standard of medical respirators, and have very high theoretical and industrial practical values.