The invention belongs to the technical field of phenylfluorone compounds and provides a 9,9'-(3,3'-dihydroxy-4,4'-diphenylether)bifluorone
reagent and a preparation method and application thereof to solve the problem that an existing phenylfluorone compound having non-ideal sensitivity and selectivity is unable to serve as a fluorescent
reagent in the fluorescent detection of
metal ions.Two pyranoid ring structural units are introduced in molecules, the quantity of
metal ion coordination sites is increased by one time, the ability of the
reagent to coordinate with
metal ions is enhanced, and the fluorescent reagent good in sensitivity and selectivity is obtained.The preparation method is simple, the cost is low, properties are stable, a
detection limit in
nickel determination by fluorophotometric
quenching process in a basic medium in the presence of a surfactant is lower that of other reagent
quenching processes,
cobalt determination by
spectrophotometry is higher than other fluorone reagent processes in sensitivity, and a
detection limit in
cobalt determination by discoloring
spectrophotometry is lower than that of a process using other fluorone reagents.This reagent has high detection speed for
nickel and
cobalt ions and is good in selectivity, high in sensitivity and high in complex stability.