34results about How to "Increase chlorogenic acid content" patented technology

The invention relates to honeysuckle tea, in particular to honeysuckle micro powder tea, and provides honeysuckle micro powder tea with a particle size of 18 micrometers. The honeysuckle micro powder tea is characterized in that the tea contains as high as more than or equal to 5 percent of chlorogenic acid, has pale green color and faint scent smell, and avoids precipitates and granular sensation after being infused. A production method comprises the following steps of: picking fresh flowers, green-removing by using microwaves, quick freezing and cooling, performing secondary drying, crushing twice and refrigerating at low temperature. Due to the function of clearing heat and detoxicating and resisting bacteria in broad spectra, the honeysuckle micro powder tea has excellent inherent quality and shape characteristic.

The invention relates to a method for extracting and separating chlorogenic acid from chrysanthemum. The method comprises the following steps: after water-removing is performed to a chrysanthemum, the raw material is lixiviated with solvent under the ultrasonic or microwave action, the crude extract of the chlorogenic acid is obtained after through solid-liquid separation, and then the chlorogenic acid pure product is obtained after through resin adsorption, separation and elution. The solvent is water, organic solvent or organic solvent-water mixed liquor. The water-removing is performed to the chrysanthemum, and the chrysanthemum is lixiviated with organic solvent or water at assistance of ultrasonic sound and microwave. Therefore, the yield of the chlorogenic acid can be improved, the production cycle is reduced, the chlorogenic acid pure product is obtained not the mixture with other ingredients, and the chlorogenic acid monomer is extracted form one chrysanthemum. Therefore, the range of the raw material of the chlorogenic acid can be extended, especially for the chrysanthemum in Canada, the chrysanthemum can turn the harmful into the beneficial and waste into valuable after development and utilization, and the invention has good economic, social and environmental benefits.

The invention provides a method for extracting chlorogenic acid and caffeic acid from coffee beans. The whole set of method is put forward for extracting high-purity chlorogenic acid and caffeic acid from coffee beans by conducting degreasing, conducting pyruvic acid water extraction, making the mixture pass through large-hole adsorption resin, conducting concentration, conducting extraction through ethyl acetate, conducting decoloration through activated carbon, and conducting crystallization and recrystallization. The requirement of the whole separation process for environment conditions is not high, the separation time is short, and purity is the highest. Separation materials can be easily obtained and are cheap, the separation operation process is simple and easy to control, crystallization and recrystallization are adopted, the purification efficiency is high, and yield is high.

The invention relates to a method for seed selection of an excellent honeysuckle flower Lonicera japonica Thunb II variety and is suitable for seed selection of a novel honeysuckle flower variety. The method comprises the following steps of collecting branches of a large chicken feet-type farm-variety plant in the main honeysuckle flower producing area in the Pingyi county of the Shandong province, carrying out irradiation mutagenesis by 15 to 25Gy of <60>Co-gamma rays, carrying out cottage and transplant, carrying out single-plant seed selection of variant individuals, carrying out cottage and transplant to obtain a plant system, and carrying out variety comparison and production test. The novel Lonicera japonica Thunb II plant has a short, thick and straight limb, a large amount of branches, long circle-shaped leaves, sparse and short pubescence at leaf veins and leaf edges, internode length of 2.5 to 7cm, average flower bud length of 4.16cm, a large amount of flowers, a long bud period, compact pier piles, concentrated buds, chicken feet-shaped flowers and an early flowering phase, can be picked easily, and is suitable for close planting. Compared with the original large chicken feet-type farm-variety, the honeysuckle flower Lonicera japonica Thunb II obtained by the method has a honeysuckle flower medicinal material yield improved by 20.2%, chlorogenic acid content improved by 17.95% and galuteolin content improved by 15.20%.

The invention discloses a preparation method for extracting chlorogenic acid from honeysuckle. The preparation method is characterized by comprising the following steps of: a, preparing a raw medicinal material, namely the honeysuckle, cleaning and drying the honeysuckle, and smashing the honeysuckle into honeysuckle powder of 10-30 meshes; b, extracting the honeysuckle powder obtained in the step a for 2-5 times with each extraction time for 1-3 hours, and combining the extracts; c, adjusting the pH value of the extract obtained in the step b to 2-6, then adsorbing the extract by macroporous resin, diluting the extract by ethanol of 70-80 percent and 50-60 percent, and combining diluted liquids to obtain a rough extracted object; and d, adjusting the pH value of the rough extracted object obtained in the step c, then extracting, separating, concentrating and drying the rough extracted object to obtain the finished product chlorogenic acid. The preparation method has the advantages that the technology is simple, the extraction temperature is low, effective components cannot be damaged, the extracted chlorogenic acid content is high, and the treating effect is better.

The invention discloses a high-quality chrysanthemum flower planting method. The planting method includes the following steps of preparing chrysanthemum flower cutting seedlings, preprocessing the cutting seedlings, transplanting and field planting of the cutting seedlings and management after field planting. Special culture and preprocessing are carried out on the cutting seedlings, the constitution features of the cutting seedlings are improved well, the arch yield of chrysanthemum flowers is increased by 35-45%, the flavonoid content in the chrysanthemum flowers is increased by 30-35%, the chlorogenic acid content is increased by 25-35%, the number of flower heads per plant is increased, and the chrysanthemum flowers have high ornamental value and medicinal value.

The invention relates to the technical field of Chinese herbal medicines, in particular to a honeysuckle extraction method, honeysuckle dry extract powder, honeysuckle detoxification capsules and a preparation method thereof. The honeysuckle extraction method comprises the following steps: (1) first decoction; (2) second decoction; (3) decompression and concentration; (4) vacuum drying. Accordingto the invention, a new vacuum dynamic low-temperature extraction method is adopted to ensure complete extraction of antibacterial active ingredients, improve the curative effect of medicines and theutilization rate of medicinal materials, the influencing factors are investigated to optimize experiments, the finally extracted honeysuckle dry extract powder has a high chlorogenic acid content, andthe extraction method is simple, effective, and easy to popularize. In addition, the invention further provides detoxification capsules taking the honeysuckle dry extract powder as a monarch drug, and the detoxification capsules have reasonable compatibility in a whole formula, have a high curative effect on clearing away heat and toxic materials and treating cold, and have a mild medicinal property and are easy to be absorbed by human bodies.

The invention discloses a glabrous sarcandra herb injection and a preparation method thereof. The injection is a sterilized aqueous solution prepared by extracting glabrous sarcandra herb and is characterized in that every milliliter of the solution contains not less than 100 mu g of rosmarinic acid; every milliliter of the solution contains not less than 500 mu g of chlorogenic acid and cryptochlorogenic acid, wherein the ratio of the chlorogenic acid to the cryptochlorogenic acid is (2:1)-(5:4); and the pH value of the solution is 5.0-5.5. The injection is stable in quality.

The invention provides Shuguan granules. The Shuguan granules are prepared from the following raw material medicines by mass: 640.8 g of radix polygoni multiflori preparata, 480 g of radix salviae miltiorrhizae, 640.8 g of rhizoma polygonati preparata, 480 g of rhizoma chuanxiong, 480 g of herba epimedii, 480 g of flos carthami and 320.4 g of vinegar-processed trogopterus dung; 85% ethanol is added for granulation, an appropriate amount of drinking water is added, ingredients are constituted by sucrose and dextrin, and the mass ratio of the sucrose to the dextrin is (1 to 2):(1 to 2). The invention further discloses a preparation method of the Shuguan granules. Thus the obtained effective component, namely stibene glucoside, in the radix polygoni multiflori preparata is remarkably improved, the content of free anthraquinone is decreased, and meanwhile, the toxic and side effects of the Shuguan granules are reduced by the greatest extent, so that the quality of the Shuguan granules is guaranteed.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a tobacco chlorogenic acid synthesis gene NtHQT and an application thereof. The full length of the gene is 4086 bp. The gene comprises an intron and two exons, and the length of a coding region is 1128 bp. Transferase encoded by the tobacco chlorogenic acid synthesis gene NtHQT contains 375 aminoacids. On the basis of the existing tobacco genetic engineering, an inventor clones to obtain a new tobacco HQT gene (NtHQT) related to the content of chlorogenic acid. In the further functional verification process, the inventor performs overexpression on the gene, and the result shows that after the gene is overexpressed, the chlorogenic acid content in a new transgenic plant is obviously improved. The result further proves that the gene is indeed related to the content of chlorogenic acid in tobacco leaves. Based on the result, a certain application foundation and reference can be laid forquality regulation and new tobacco variety cultivation in the tobacco growth process.

The invention discloses a probiotic feed with high viable count prepared from baijiu distillers' grains by performing fermentation with lactobacillus plantarum and a preparation method thereof. The probiotic feed with high viable count is prepared from the following raw materials in parts by weight: 70-85 parts of dry distiller's grains, 15-30 parts of folium eucommiae powder, 0-3.0 parts of ammonium persulphate, 0.5-2 parts of fructo-oligosaccharides, and 0.5-2 parts of inulin. The preparation method of the probiotic feed with high viable count comprises the following steps: adding the foliumeucommiae powder, the ammonium persulphate, the fructo-oligosaccharides and the inulin into the dry distiller's grains, carrying out uniform mixing, moistening the mixture, and carrying out steaming;and then, inoculating the steamed mixture with a bacterial suspension of lactobacillus plantarum after completion of the steaming, and carrying out anaerobic solid-state fermentation so as to obtainthe probiotic feed with high viable count. The preparation method of the probiotic feed with high viable count utilizes relatively strong carbohydrate decomposition ability of the lactobacillus plantarum to degrade macromolecular carbohydrates in the distiller's grains and the folium eucommiae, so that, contents of crude proteins, small peptides, oligosaccharides and monosaccharides in the feeds are increased; moreover, content of chlorogenic acid in the feed is also increased by performing fermentation on the folium eucommiae powder. In addition, prebiotics are also added, so that, proliferation of the lactobacillus plantarum in animal intestines is also increased.

The invention relates to a germplasm improvement method for traditional Chinese medicinal materials. The germplasm improvement method comprises the steps of A germplasm selection, B germplasm improvement, C improved germplasm identification and screening, D improved germplasm rooting, E rooted seedling domestication and F improved germplasm seed obtaining. According to the method, colchicine is used for conducting polyploid induction on ainsliaea fragrans sterile adventitious buds, then screening and purification culture are conducted through a morphological identification method and a chromosome tabletting method, the improved ainsliaea fragrans germplasm is obtained, and the obtaining rate is 19%; according to the obtained improved ainsliaea fragrans germplasm, stems are obviously thickened, leaves are obviously thickened, the single plant biomass is 1.9 times higher than that of an original female parent, the yield per mu is 1.6 times higher than that of the original female parent, meanwhile, the chlorogenic acid content is 1.3 times higher than that of the female parent, and the protocatechuic acid content is 2.2 times higher than that of the original female parent; and the obtained high-quality germplasm has genetic stability, and plants planted through the seeds still have fertility.

The invention discloses a glabrous sarcandra herb injection and a preparation method thereof. The injection is a sterilized aqueous solution prepared by extracting glabrous sarcandra herb and is characterized in that every milliliter of the solution contains not less than 100 mu g of rosmarinic acid; every milliliter of the solution contains not less than 500 mu g of chlorogenic acid and cryptochlorogenic acid, wherein the ratio of the chlorogenic acid to the cryptochlorogenic acid is (2:1)-(5:4); and the pH value of the solution is 5.0-5.5. The injection is stable in quality.

The invention belongs to the field of tobacco genetic engineering, in particular to tobacco transcription factor genes NtFAMA and its application patent application. The base sequence of the gene is shown in SEQ ID NO.1. Tobacco transcription factor gene NtFAMA It consists of 287 amino acid residues, of which the amino acid transporter domain at the 91-142nd amino acid is conserved. This protein is related to the content of chlorogenic acid in plant leaves. After reducing the expression of this protein, the content of chlorogenic acid in leaves is significantly increased. The present invention is directed to specific tobacco transcription factors by NtFAMA Preliminary research on , found that it was highly correlated with the content of chlorogenic acid in tobacco, and after the gene was silenced, the content of chlorogenic acid in tobacco was significantly increased. Based on this characteristic, it can provide a certain application basis and reference for the cultivation of new tobacco varieties with reasonable chlorogenic acid content.

The invention discloses a method for controlling corn armyworm by using nanometer silicon quantum dots, and belongs to the technical field of nanometer material prevention and control of crop pests. The method of using nano-silicon quantum dots in the present invention to prevent and control corn armyworm, the method is to prepare nano-silicon quantum dots as an aqueous solution of nano-silicon quantum dots, and then apply them to plant roots or leaves as plant fertilizers; wherein the The concentration of the aqueous solution of the nano-silicon quantum dots is 10-150mg / L; the size of the nano-silicon quantum dots is 3-8nm. The method of the present invention not only improves the effect of conventional silicon fertilizers on plant stress resistance, but also directly improves the direct insecticidal effect of nano-silicon and the improvement of the plant's own chemical defense ability; by spraying different concentrations of nano-silicon quantum dots The optimal spraying amount of nano-silicon quantum dots was established in the growth and development experiment of armyworm.

The invention discloses a processing method for honeysuckles, which includes the steps of: 1) mixing and pan-firing picked honeysuckles and white soft inner peel of pomelo, wherein during the pan firing, initial temperature is maintained at 65 DEG C, and then the temperature is increased to 75-85 DEG C within 2-5 min, and the raw materials is continuously pan-fired for 10-20 min with temperature maintained; 2) sieving the pan-fired substances and removing the white soft inner peel of pomelo, and air-drying the honeysuckles with air humidity being 1-3% at room temperature for 2-3 h. By means of the method, water content of the honeysuckles is lower than 7%, and the honeysuckles has good appearance, is free of blackening and is high in content of chlorogenic acid; the dried white soft inner peel of pomelo is mixed with the honeysuckles for mixing and pan firing, wherein the dried white soft inner peel of pomelo can effectively absorb water vapor generated during the pan-firing process, so that the pan firing process is carried out under a dry environment, and the honeysuckles has good quality and appearance. Furthermore, the method is short in time, is low in energy consumption and is low in cost.

The invention discloses a traditional Chinese medicine composition for treating acne as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 200 to 600 parts of honeysuckle flowers, 100 to 300 parts of chrysanthemum flowers, 100 to 300 parts of herba taraxaci and 50 to 150 parts of liquorice roots. Further, the invention also provides a preparation method of the traditional Chinese medicine composition. A treatment mode of combining a microbiological degradation with a fast elution method is used for treating medicinal materials for the first time; the treatment time is shortened; the heavy metal content is reduced; the pesticide residue problem is solved. In addition, a microwavefixation method is used for treating and processing the medicinal materials; the enzyme activity and the chlorogenic acid content are improved. Experiments prove that the traditional Chinese medicinecomposition can radically treat the acne; the raw material varieties are few; the preparation process is simple; the quality is controllable; the active pharmaceutical ingredients take a synergisticeffect; the efficacies of clearing away heat and toxic materials, dispelling wind-heat, relaxing spasm and relieving pain can be achieved. The traditional Chinese medicine composition provided by theinvention provides a convenient and effective treatment method for acne treatment.

The invention relates to an application of a traditional Chinese medicine composition in preparation of a drug for improving livestock production performance. A method comprises the steps of: adding an auxiliary material to the traditional Chinese medicine composition, boiling the materials for granulating, and performing split charging to prepare the drug; wherein, the traditional Chinese medicine composition comprises radix bupleuri, hypericum japonicum, Artemisia capillaris, dandelion, licorice and desmodium. The traditional Chinese medicine composition has the functions of soothing liver and regulating qi, clearing liver and disinhibiting dampness, and has good water solubility, and can be used for effectively improving the production performance of livestock and poultry.

The invention provides a Shuguan granule, which is prepared by extracting the following raw materials: Polygonum multiflorum 640.8g, Salvia miltiorrhiza 480g, Polygonatum 640.8g, Rhizoma Chuanxiong 480g, Epimedium 480g, Safflower 480g, Vinegar-made Wulingzhi 320.4g g, 85% ethanol granulation, appropriate amount of drinking water, wherein the auxiliary material is composed of sucrose and dextrin, the mass ratio of sucrose and dextrin is 1‑2:1‑2, and its preparation method is disclosed, so that the obtained Polygonum multiflorum The active ingredient stilbene glycosides is significantly improved, and the free anthraquinone content is reduced, while minimizing the toxic and side effects of the product, thereby ensuring the quality of the product.

The invention relates to wild honeysuckle flower health protection teabags comprising the following raw materials in parts by weight: 10-20 parts of wild honeysuckle flower, 20-30 parts of wild honeysuckle flower leaves, 10-20 parts of wild honeysuckle flower twigs, 15-25 parts of liquorices, 10-20 parts of tea leaves and 10-20 parts of stevia rebaudianum. A making method of the wild honeysuckle flower health protection teabags comprises the following steps of: screening to remove impurities; washing by using water, and then airing or air-drying; respectively smashing, screening, collecting raw material granules within a range of 60-100 meshes for later use; sufficiently mixing and uniformly stirring according to the parts by weight; irradiating by using ultraviolet rays for disinfection and sterilization; loading into filter paper bags according to the specification of 2-5 grams per bag, and carrying out heat-sealing; respectively loading into packaging boxes, and then checking and warehousing. The wild honeysuckle flower health protection teabags have the beneficial effect of playing a role in enhancing immunity when people drink tea; in addition, the wild honeysuckle flower health protection teabags disclosed by the invention have the advantages of readily available raw material sources, lower cost and price, capability for large-scale production and suitability for ordinary people to drink and can generally increase the level of the physical health of people.

The invention discloses a probiotic feed with high viable bacteria count and a method prepared by fermenting liquor distiller's grains with Lactobacillus plantarum. The probiotic feed with high viable count is made of the following raw materials in parts by weight: dried distiller's grains 70-85 , Eucommia leaf powder 15-30, ammonium sulfate 0-3.0, fructo-oligosaccharide 0.5-2 and inulin 0.5-2. The preparation method is as follows: add Eucommia ulmoides leaf powder, ammonium sulfate, fructooligosaccharide and inulin to the dried distiller's grains, mix evenly, and steam the material with moist water; after steaming, inoculate Lactobacillus plantarum suspension for anaerobic solid-state fermentation to obtain High viable count probiotic feed. The invention utilizes the strong ability of Lactobacillus plantarum to decompose sugars, degrades the macromolecular sugars in distiller's grains and Eucommia leaves, and increases the content of crude protein, small peptides, oligosaccharides and monosaccharides in feed; and after Eucommia leaves are fermented, It also increases the content of chlorogenic acid in the feed, while adding prebiotics also increases the proliferation of Lactobacillus plantarum in the intestinal tract of animals.

The invention relates to the technical field of coreopsis tinctoria processing, in particular to a coreopsis tinctoria tablet and a preparation method of the coreopsis tinctoria tablet. The preparation method of the coreopsis tinctoria tablet includes the steps that fresh coreopsis tinctoria is dried in a microwave vacuum mode, smashed and sieved, superfine coreopsis tinctoria powder is obtained, an appreciate amount of the superfine coreopsis tinctoria powder, auxiliary materials and food additives are evenly mixed, pelletized and compressed into a tablet, and then the coreopsis tinctoria tablet is prepared. The prepared coreopsis tinctoria tablet has the advantages of being convenient to carry and eat; in the coreopsis tinctoria tablet prepared through the method, one gram of dry coreopsis tinctoria per gram contains 8.5 milligrams to 8.8 milligrams of chlorogenic acid, and compared with coreopsis tinctoria prepared through an existing drying method, the content of chlorogenic acid in coreopsis tinctoria is increased; in addition, the prepared coreopsis tinctoria tablet can reserve a large number of flavor compounds of coreopsis tinctoria and has the advantage of being strong in fragrance; the superfine coreopsis tinctoria powder can be more easily absorbed into the human body, in other words, the coreopsis tinctoria has the advantage of being easy to absorb.

The invention relates to burdock leaf tea with high chlorogenic acid content and a preparation method and application thereof, and the method comprises the following steps: (1) selecting burdock leaves: selecting fresh burdock leaves with the length of 5-8 cm; cleaning the burdock leaves, and draining; (2) withering; (3) performing low-temperature and ultrahigh-pressure pretreatment; (4) fermenting; (5) performing low-temperature ultrahigh-pressure sterilization; (6)performing rotary evaporation and concentration; and (7) drying at low temperature to obtain the burdock leaf tea with high chlorogenic acid content. Based on the characteristic of instability of chlorogenic acid, all the steps are selected to be operated at low temperature, so that the structure of chlorogenic acid is not damaged, the content of chlorogenic acid contained in burdock leaves is high, and the burdock leaf tea product with the high content of chlorogenic acid is obtained through soaking. And the two-time low-temperature ultrahigh-pressure and fermentation technologies can enable effective substances such as chlorogenic acid and polysaccharide to be leached from the burdock leaves to the greatest extent.

The present invention relates to Chinese medicine preparation, and is especially the preparation process and application of Chinese medicine preparation for treating vocal nodules and polyp of vocal cord. The preparation process of the present invention is stable, practical, high material utilizing rate and high effective component extracting rate, and the Chinese medicine preparation has high chlorogenic acid content. The Chinese medicine preparation has the functions of promoting blood circulation to disperse blood clots, lowering blood viscosity, lowering fibrinogen content and platelet adhesiveness, and inhibiting proliferation of granulation tissue.

The invention discloses a production method of a coffee fresh fruit extract. The production method comprises the following steps: 1) crushing coffee fresh fruits into coffee pulp; 2) dissolving pectinase, hemicellulase and beta-glucolase, and mixing to obtain a compound enzyme; (3) adding the coffee fruit pulp, compound enzyme and water into a first-stage enzymolysis extraction tank, performing first-stage enzymolysis, performing second-stage high-temperature extraction and third-stage high-temperature extraction in sequence, and collecting extract; 4) centrifugally discharging slag from the extract, cooling the obtained extract, and filtering; 5, the extract liquid is subjected to freeze concentration, canning or freeze drying, and the coffee fresh fruit extract.The coffee fresh fruit extract is prepared from the coffee fresh fruit as the raw material, the compound enzyme and a multi-stage tank are effectively combined for extraction, the coffee fresh fruit extract is processed through low-temperature processing technologies such as freeze concentration, the fragrance of the coffee fresh fruit is prominent, and effective ingredients can be more sufficiently extracted.

The invention provides a method for extracting chlorogenic acid and caffeic acid from coffee beans. The whole set of method is put forward for extracting high-purity chlorogenic acid and caffeic acid from coffee beans by conducting degreasing, conducting pyruvic acid water extraction, making the mixture pass through large-hole adsorption resin, conducting concentration, conducting extraction through ethyl acetate, conducting decoloration through activated carbon, and conducting crystallization and recrystallization. The requirement of the whole separation process for environment conditions is not high, the separation time is short, and purity is the highest. Separation materials can be easily obtained and are cheap, the separation operation process is simple and easy to control, crystallization and recrystallization are adopted, the purification efficiency is high, and yield is high.

The invention relates to a method for improving germplasm of traditional Chinese medicinal materials. The steps include: A germplasm selection, B germplasm improvement, C improved germplasm identification and screening, D improved germplasm rooting, E rooted seedling domestication and F improved germplasm seeds Obtained; the present invention uses colchicine to induce polyploidy of aseptic adventitious buds of Xingxiang rabbit ear wind, and then screens and purifies the improved Xingxiang rabbit ear wind germplasm through morphological identification method and chromosome pressing method, and the acquisition rate is 19%; the improved Xingxiang rabbit ear wind germplasm obtained by the present invention has obvious thickening of stems and obvious thickening of leaves, and the biomass per plant is 1.9 times higher than that of the original female parent, and the yield per mu is 1.6 times higher than that of the original female parent , at the same time, the content of chlorogenic acid is 1.3 times higher than that of the female parent, and the content of protocatechuic acid is 2.2 times higher than that of the original female parent; the high-quality germplasm obtained by the present invention has genetic stability, and the plants planted by seeds still have the potential Fertility.

The invention discloses a method for increasing the content of chlorogenic acid in chrysanthemum inflorescences. The method comprises the following steps: (a) putting picked fresh chrysanthemum inflorescences into a culture room, and irradiating by using ultraviolet rays UV-B at irradiation intensity of 50-600muW / cm<2> for 15-120 minutes under the conditions that the temperature is 20-25 DEG C and the humidity is 50%-80%; (b) transferring the irradiated inflorescences into a culture box and performing dark culture for 12 hours under the conditions that the temperature is 20-25 DEG C and the humidity is 50%-80% in the box; (c) performing fixation on the inflorescences processed in the step (b) in a drying machine at 100-150 DEG C for 8-10 minutes, and drying at 50-55 DEG C until the weight is constant. According to the preparation method, the process is simple, the environment-friendly effect is achieved, the safety coefficient is high, the production cycle is short, the cost is low, the benefit is high, the operation controllability is good and the chlorogenic acid is suitable for large-scale industrialized production.

The invention discloses a chlorogenic acid-rich wine. A chlorogenic acid extract is added into grape pulp for joint fermentation, and the chlorogenic acid extract is prepared from aronia melanocarpa fruits. The chlorogenic acid extract is prepared by compound extraction of multiple enzymes. The invention also discloses a production method of the chlorogenic acid-rich wine. According to the invention, no sulfur-containing compound needs to be added in the production process of the wine, and the wine has a deep purple red appearance, strong, pure, complex and elegant aroma, smooth taste, light bitterness, full wine body, lasting aftertaste, strong typicality and high chlorogenic acid content, has antibacterial and antioxidant effects after frequent drinking, and has a preventive effect on cardiovascular and cerebrovascular diseases.

The invention provides a method for increasing the content of effective ingredients of medicinal chrysanthemum by virtue of light irradiation. The method comprises the following steps: (1) setting a red light source over a planting field of the medicinal chrysanthemum; and (2) starting the light source at fixed time within the growth period of the medicinal chrysanthemum to irradiate plants. The invention also provides the medicinal chrysanthemum cultivated by using the method, and compared with the medicinal chrysanthemum which is not irradiated by the light source, the medicinal chrysanthemum has the advantages that the content of chlorogenic acid serving as one of the effective ingredients is increased by 25%, the content of 3,5-O-dicaffeoyl quinic acid is increased by 18%, and the content of galuteolin is increased by 24%. By using the method provided by the invention, the content of the effective ingredients in the medicinal chrysanthemum can be greatly increased; and the method is simple and feasible, short in light irradiation time, low in energy consumption and favorable in popularization prospect and economic value.