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Method for extracting and separating chlorogenic acid from chrysanthemum

A technology of chlorogenic acid and goldenrod, which is applied to chemical instruments and methods, medical preparations containing active ingredients, and pharmaceutical formulas, etc., can solve the problems of inability to extract a single component and low extraction rate, and achieve good economic benefits and improve The effect of extraction efficiency and shortening production cycle

Inactive Publication Date: 2009-06-03
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention provides a new method of producing chlorogenic acid from Solidago sagoides, which effectively solves the problem of low extraction rate and inability to extract a single group of score question

Method used

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  • Method for extracting and separating chlorogenic acid from chrysanthemum
  • Method for extracting and separating chlorogenic acid from chrysanthemum
  • Method for extracting and separating chlorogenic acid from chrysanthemum

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Embodiment 1: extraction treatment and analysis (flower or leaf)

[0036] Thermo company Hypersil ODS C18 (250mm × 4.6mm, 5μm) chromatographic column; mobile phase A is disodium hydrogen phosphate buffer solution (10mmol / L), mobile phase B is methanol; mobile phase ratio is: mobile phase B in 0 ~ 15min Keep 25%; flow rate: 1mL / min; column temperature: 30°C; injection volume: 20μL; detection wavelength 326nm, time 12min.

[0037] Chlorogenic acid standard solution (0.44mg / mL) was injected to obtain image 3 The HPLC figure of each treatment sample; after the filter membrane precision filtration of the leach solution of each treatment sample, the operation of carrying out HPLC determination is the same as that of the standard solution.

[0038] Fix raw materials: the temperature during processing is 100-300°C, and the processing time is 5 seconds to 60 minutes.

[0039] Weigh 1g of solidago powder, add 20mL of water with pH 4.0, and conduct ultrasonic assisted leaching ...

Embodiment 2

[0045] Add the solid-liquid ratio of solid-liquid ratio of 1:20 (W / W) into the ethanol aqueous solution of pH 6.0, ethanol: water 1:5 (W / W), and leaching for 3 hours under the action of microwave. After solid-liquid separation, concentration, separation and purification to obtain refined chlorogenic acid products.

Embodiment 3

[0047] Add the leaves of Solidago japonica treated with solid-liquid ratio 1:4 (W / W) into the ethanol aqueous solution with pH 3.0, ethanol: water-1:0.1 (W / W), and extract under the action of microwave for 6 hours , after solid-liquid separation, the crude extract is passed through an ion exchange column, eluted with water and methanol, and the eluate is concentrated to recover the solvent to obtain the chlorogenic acid product.

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Abstract

The invention relates to a method for extracting and separating chlorogenic acid from chrysanthemum. The method comprises the following steps: after water-removing is performed to a chrysanthemum, the raw material is lixiviated with solvent under the ultrasonic or microwave action, the crude extract of the chlorogenic acid is obtained after through solid-liquid separation, and then the chlorogenic acid pure product is obtained after through resin adsorption, separation and elution. The solvent is water, organic solvent or organic solvent-water mixed liquor. The water-removing is performed to the chrysanthemum, and the chrysanthemum is lixiviated with organic solvent or water at assistance of ultrasonic sound and microwave. Therefore, the yield of the chlorogenic acid can be improved, the production cycle is reduced, the chlorogenic acid pure product is obtained not the mixture with other ingredients, and the chlorogenic acid monomer is extracted form one chrysanthemum. Therefore, the range of the raw material of the chlorogenic acid can be extended, especially for the chrysanthemum in Canada, the chrysanthemum can turn the harmful into the beneficial and waste into valuable after development and utilization, and the invention has good economic, social and environmental benefits.

Description

technical field [0001] The invention relates to a method for extracting chlorogenic acid from goldenrod, in particular to a method for leaching chlorogenic acid from goldenrod goldenrod with the assistance of deenzyming, ultrasonic waves or microwaves and purifying the chlorogenic acid with macroporous resin. Background technique [0002] The whole plant of goldenrod is used as medicine and is a nectar source plant. There are 4 species native to China: Solidago solidago (S.virgaurea L.), Solidago solidago (ssp.dahurica), Solidago pacifica (S.pacifica Jucz.) and Solidago solidago (S.decurrens Lour.). S. virgaurea is distributed in forest margins and shrubs at an altitude of 1200-2620m in the Altai Mountains and Tianshan Mountains of Xinjiang, my country. This species is widely distributed in Russia, Caucasus, Mongolia and Europe. Invasive species in my country include Canadian goldenrod (S.canadensis), fragrant goldenrod (S.odorata), giant goldenrod (S.gigantean; S.gigantea ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/56A61K36/28
Inventor 陈育如刘虎姜中玉陈雁刘军利
Owner NANJING NORMAL UNIVERSITY
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